Genes associated with resistance to wheat yellow rust

ABSTRACT

An isolated nucleic acid encoding a nucleotide-binding and leucine-rich repeat (NLR) polypeptide including a zinc-finger BED domain, wherein expression of the NLR polypeptide in a plant confers or enhances resistance of the plant to a fungus.

FIELD OF THE INVENTION

The invention relates to genes associated with disease resistance inplants.

BACKGROUND OF THE INVENTION

Crop diseases pose a threat to global food security. Genetic resistancecan reduce crop losses in the field and can be selected using molecularmarkers. However, it often breaks down due to changes in pathogenvirulence as experienced for the wheat yellow (stripe) rust fungusPuccinia striiformis f. sp. tritici (PST). This highlights the need to(i) identify genes that alone or in combination provide broad-spectrumresistance and (ii) increase our understanding of their molecularmechanisms.

NLRs are intracellular receptors which induce cell death upon pathogenrecognition to prevent disease spread throughout the plant. Differentmodes of action for this gene family have been discovered over the pasttwenty years. The NB-ARC domain is the signature of the NLRs which inmost cases carry additional Leucine Rich Repeats (LRR) at theC-terminus. Recent in silico analyses have identified NLRs withadditional ‘integrated’ domains at different positions of the genestructure. These include zinc-finger BED domains (BED-NLRs) which arewidespread across Angiosperm genomes and can confer resistance tobacterial blast in rice (Xa1).

In plant immunity, NLRs act as intracellular immune receptors thattrigger a series of signalling steps ultimately leading to cell deathupon pathogen recognition, preventing the disease spread throughout theplants. The NB-ARC domain is the hallmark signature of the NLRs which inmost cases carry leucine-rich repeats (LRR) at the C-terminus. Recent insilico analyses have identified NLRs with additional ‘integrated’domains, including zinc-finger BED domains (BED-NLRs). The BED domainfrom the DAYSLEEPER protein binds DNA in Arabidopsis, however whetherBED domains from BED-NLRs conserved this function is unknown. BED-NLRsare widespread across Angiosperm genomes and this architecture providesresistance to bacterial blast in rice through Xa1.

The genetic relationship between Yr5 and Yr7 has been debated for almost45 years. Both genes map to chromosome arm 2BL in hexaploid wheat(Triticum aestivum) and were hypothesized to be allelic, and closelylinked with YrSP. While Yr5 confers resistance to almost all tested PSTisolates worldwide, both Yr7 and YrSP have been overcome in the fieldfollowing wide deployment (Table 1) and each display a differentrecognition specificity.

SUMMARY OF THE INVENTION

According to an aspect of the invention is provided an isolated nucleicacid encoding a nucleotide-binding and leucine-rich repeat (NLR)polypeptide comprising a zinc-finger BED domain, wherein expression ofthe NLR polypeptide in a plant confers or enhances resistance of theplant to a fungus, for example wheat yellow (stripe) rust fungusPuccinia striiformisi f. sp. tritici.

Further aspects and embodiments are as defined in the appended claimsand in the detailed description below.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1. Yr5 and YrSP are allelic and paralogous to Yr7

(A) Left-Pictures of wild-type and selected EMS-derived susceptiblemutant lines for Yr7, Yr5 and YrSP (Tables 2-3) inoculated with PSTisolate 08/21 (Yr7), PST 80/11 (Yr5), PST 134 E16 A+ (YrSP). Candidategene structures, with mutations shown with black bars, identified byRenSeq and their predicted effects on the translated protein are shownon the right. (B) Schematic representation of the physical and geneticinterval of the Yr loci. Schematic representation of chromosome 2BL andthe Yr loci is shown in grey with previously published SSR markers shownin black. Markers that we developed to confirm the genetic linkagebetween this locus and the candidate contigs are shown with black markson the close-up underneath the chromosme. Yr loci mapping intervals aredefined by the black horizontal lines. A more detailed genetic map isshown in FIG. 5.

FIG. 2: Yr7 and Yr5/YrSP encode integrated BED-domain resistance genes

(A) Schematic representation of the Yr7/Yr5/YrSP protein domainorganisation. BED domains are highlighted in black, NB-ARC domains indark grey, LRR motifs from NLR-Annotator in grey and manually annotatedLRR motifs xxLxLxx in light grey. The sequence identity between YrSP andYr5 is shown in light grey. Asterisks point the EMS-induced mutationpositions. The plot shows the degree of amino acid conservation (50 AArolling average) between Yr7 and Yr5 at the protein level based on theconservation diagram produced by Jalview (2.10.1) alignment viewer.Regions that correspond to the conserved domains have matching greyscaleon the line. The amino acid changes between Yr5 and YrSP are annotatedon the YrSP protein. (B) Five Yr5/YrSP haplotypes were identified inthis study. Polymorphism are highlighted across the protein sequencewith grey vertical bars for polymorphisms shared by at least twohaplotypes and light grey vertical bars showing polymorphism that areunique to the corresponding haplotype. Matching greyscale across proteinstructures illustrate 100% sequence conservation.

FIG. 3: BED domains from BED-NLRs and non-NLR proteins are distinct

(A) Table representing the NLR counts in the syntenic region acrossgenomes (see FIG. 6) showing their expansion in the Triticeae and theidentification of BED-BED-NLRs. (B) WebLogo(http://weblogo.berkeley.edu/logo.cgi) diagram showing that the two BEDdomains from BED-BED-NLRs, BED-I and -II, are distant and only thehighly conserved amino acids that define the BED domain (red bars) areconserved between the two types. (C) Gene structure most commonlyobserved for BED-NLRs and BED-BED-NLRs shows that BED is in most casesencoded by a single exon. (D) Neighbour-net analysis based onuncorrected P distances obtained from alignment of 153 BED domains(amino-acid sequences) extracted from the 108 BED-containing proteins(including 25 NLRs) from RefSeq v1.0. BED domains from NLRs located inthe syntenic region defined in FIG. 6 and BED domains from Xal and ZBEDfrom rice. BED_I and II clades are highlighted with the arc line, BEDdomains from the syntenic regions not related to either of these typesare in dark grey. BED domains derived from non-NLR proteins are in blackand BED domains from BED-NLRs outside the syntenic region are in lightgrey. For a better view, we removed the identifiers (see FIG. 8 for thedetailed network). Seven BED domains from non-NLR proteins were close toBED domains from BED-NLRs.

FIG. 4: Identification of candidate contigs for the Yr loci usingMutRenSeq

Annotated screen capture of RenSeq reads from the wild-type and mappingof EMS-derived mutants to the best candidate contig identified withMutantHunter for the three genes targeted in this study. From the top tothe bottom: Vertical black lines represent the Yr loci, rectanglesdepict the motifs identified by NLR-Annotator (each motif is specific toa conserved NLR domain), while read coverage (grey histograms) isindicated on the left, e.g. [0-149], and the line from which the readsare derived on the right, e.g. CadWT for Cadenza wild-type. Verticalbars represent the position of SNP identified between the reads andreference assembly—dark grey shows C to T transitions and light grey Gto A transitions. Black boxes highlight SNP for which the coverage waslower, but still superior to the 20x threshold used here.

The top screen capture shows the Yr7 allele annotated and beforecuration from the Cadenza genome assembly (Table 4). Light grey dashedlines illustrate the actual locus and the one that was formerly de novoassembled from Cadenza RenSeq data, lacking the 5′ region containing theBED domain and thus the Cad903 mutation. This locus was the only one forwhich all seven mutant lines carried a mutation. The middle screencapture illustrates the Yr5 locus annotated from the Lemhi-Yr5 de novoassembly. The results are similar to those described above for Yr7. Thefull locus was de novo assembled.

FIG. 5: Candidate contigs identified by MutRenSeq are genetically linkedto the Yr loci mapping interval

Schematic representation of chromosome 2B from Chinese Spring (RefSeqv1.0) with the positions of published markers linked to the Yr loci andsurrounding closely linked markers that were used to define theirphysical position (grey regions). Close-up of the physical locusindicating the positions of KASP markers that were used for the mapping(vertical bars Table 10). Light grey refers to Yr7, dark grey to Yr5 andgrey to YrSP. The arrow points to the NLR cluster containing the bestBLAST hits for Yr7 and Yr5/YrSP on RefSeq v1.0. Lines link the physicalmap to the corresponding genetic map for each targeted gene (seeMethods). Values are expressed in centiMorgans.

FIG. 6: Expansion of BED-NLRs in the Triticeae and presence ofBED-BED-NLRs whose BED domains are conserved across the syntenic region

Schematic representation of the physical loci containing Yr7 andYr5/YrSP homologues on RefSeq v1.0 and its syntenic region based on genecontent across RefSeq v1.0 subgenomes and selected grass genomes. Arrowsrepresent loci. The syntenic region in other species was defined whenthree consecutive non-NLR genes had orthologues in the same ordercompared to chromosome 2BL outside the NLR cluster (see Methods). Thesyntenic region is bordered by conserved non-NLR genes (shown in lightgrey). Black arrows represent canonical NLRs and the different shades ofgrey arrows represent different types of BED-NLRs based on their BEDdomain and their relationship identified in FIG. 9. Grey lines link NLRssharing more than 80% ID across more than 80% of their aligned sequence.Brown dashed lines represent the closest BED-NLR from the Triticeae toBED_I and II found in Brachypodium (Bd3 and Bd4, respectively).

FIG. 7: The Yr loci are phylogenetically related to surrounding NLRs onRefSeq v1.0 and their orthologs

Phylogenetic tree based on translated NB-ARC domains from theNLR-Annotator. Sequences were aligned using Muscle v3.8.13 with defaultparameters and the tree was built with the MPI version of the RAxML(v8.2.9) program. Node labels represent bootstrap values for 1,000replicates. The tree was rooted at mid-point and visualized withDendroscope v3.5.9. The greyscale pattern matches the one in FIG. 3 tohighlight BED-NLRs with different BED domains. There was clearseparation between NLRs belonging to the two different clusters but thesub-clades have less support. One explanation would be that conflictingphylogenetic signals due to events such as hybridization, horizontalgene transfer, recombination, or gene duplication and loss might haveoccured in the region. Split networks allow nodes that do not representancestral species and can thus represent such incompatible and ambiguoussignals. We thus used this method in the following part of the analysisto analyse the relationship between the BED domains.

FIG. 8: Same Network as the one shown on FIG. 3 with the identifiers ofall analysed proteins.

FIG. 9: BED-NLRs and BED-containing proteins are not differentiallyexpressed in yellow rust-infected susceptible and resistant varieties

Heatmap representing the normalised read counts (Transcript Per Million,TPM) from the reanalysis of RNAseq data for all of the BED-containingproteins and BED-NLRs annotated on RefSeq v1.0. No expression is shownin white and expression levels increase from light grey to dark grey.Most BED-containing protein and BED-NLRs were not expressed at all inthe analysed data. No striking pattern was observed for those that wereexpressed: difference were observed between varieties but these wereindependent of the presence of the yellow rust pathogen.

FIG. 10: Pedigrees of selected Thatcher-derived varieties and varietiesknown to carry Yr7 based on marker data.

The size of the circle is proportional to the prevalence of the varietyin the tree. Greyscale illustrate the genotype with dark grey showingthe absence of Yr7 and grey its presence. Varieties in light grey werenot tested. Yr7 originated from Triticum durum cv. Iumillo and wasintrogressed into hexaploid wheat through Thatcher (top of thepedigree). All the varieties. Each variety positive for the Yr7 alleleis related to a parent that was also positive for Yr7.

FIG. 11: Screen capture of the mapping of the Paragon RenSeq reads tothe Cadenza NLR set showing that Paragon likely carries an identicalversion of Yr7

FIG. 12: Design of a allele-specific primer for Yr5. Yr5-Insertion PCRamplification products obtained from Yr5 donnor

Spelt and Yr5 Isogenic Lines AvocetS+Yr5 and Lemhi+Yr5, YrSP donorSpaldings Prolific and YrSP Isogenic Line AvocetS+YrSP, lines carryingalternate Yr5 alleles identified on FIG. 2 (Claire, Cadenza, Paragon),Negative controls AvocetS and Water. Molecular weight marker is the2-log ladder from New England Biolab.

DETAILED DESCRIPTION OF THE INVENTION

In a first aspect the invention relates to an isolated nucleic acidencoding a nucleotide-binding and leucine-rich repeat (NLR) polypeptidecomprising a zinc-finger BED domain, wherein expression of the NLRpolypeptide in a plant confers or enhances resistance of the plant to afungus, for example wheat yellow (stripe) rust fungus Pucciniastriiformisi f. sp. tritici.

The isolated nucleic acid may be isolated from a plant, for example anAngiosperm such as Aegilops tauschii, Brachypodium distachyon, Oryzasativa, Triticum turgidum or Triticum aestivum.

The BED domain may have an amino acid sequence corresponding to SEQ IDNO: 1 (BED-I sequenceSVVWEHFTITEKDNGKPVKAVCRHCGNEFKCDTKTNGTSSMKKHLENEHS) or a variant thereof(see for example BED-I variants and consensus sequence shown in FIG. 3A)or a functional fragment thereof.

The NLR polypeptide may comprise a leucine-rich repeat (LRR) motif at ornear the C-terminus.

The NLR polypeptide may have an amino acid sequence comprising SEQ IDNO: 2 (Yr5 protein) or SEQ ID NO: 3 (Yr7 protein), or a variant orfunctional fragment of either, including variants described herein. Forexample, the isolated nucleic acid may have a nucleotide sequencecomprising SEQ ID NO: 4 (Yr5 gene nucleotide sequence), or itscorresponding cDNA sequence, SEQ ID NO: 5 (Yr7 gene nucleotidesequence), or its corresponding cDNA sequence, or variants or functionalfragments thereof, including other alleles described herein.

Alternatively, the NLR polypeptide may have an amino acid sequencecomprising SEQ ID NO: 6 (YrSP protein) or a variant or functionalfragment thereof, including variants described herein. For example, theisolated nucleic acid may have a nucleotide sequence comprising SEQ IDNO: 7 (YrSP nucleotide sequence) or its corresponding cDNA sequence, orvariants or functional fragments thereof, including other allelesdescribed herein.

The NLR polypeptide may comprise a further zinc-finger BED domain, forexample having an amino acid sequence comprising SEQ ID NO: 8 (BED-IIsequence KAWDNFDVIEEENGQPIKARCKYCPTEIKCGPKSGTAGMLNHNKICKD) or a varianttherefore (see for example BED-II variants and consensus sequence shownin FIG. 3A) or a functional fragment thereof.

In another aspect the invention relates to a nucleotide-binding andleucine-rich repeat (NLR) polypeptide comprising a zinc-finger BEDdomain, wherein expression of the NLR polypeptide in a plant confers orenhances resistance of the plant to a fungus, for example wheat yellow(stripe) rust fungus Puccinia striiformisi f. sp. tritici. The BEDdomain may have an amino acid sequence comprising SEQ ID NO: 1 (BED-I)or a variant or functional fragment thereof

Further features of the NLR polypeptide per se of the invention may bedefined as above and herein.

In another aspect the invention relates to a vector comprising anisolated nucleic acid of the invention. The vector may furthercomprising a regulatory sequence which directs expression of the nucleicacid, for example a regulatory sequence selected from a constitutivepromotor, a strong promoter, an inducible promoter, a stress promotor ora tissue specific promoter.

In yet another aspect, the invention relates to a host cell comprising anucleic acid, an NLR polypeptide or a vector of the invention. The hostcell may be a bacterial cell, a yeast cell, plant cell or other celltype.

In another aspect, the invention relates to a method of producing atransgenic plant or plant cell comprising introducing and expressing anucleic acid or a vector according to the invention into a plant orplant cell, wherein introducing and expressing the nucleic acid orvector confers or enhances resistance of the plant or plant cell to afungal pathogen such as wheat yellow (stripe) rust fungus Pucciniastriiformisi f. sp. tritici.

The transgenic plant or plant cell may have resistance or enhancedresistance to the fungal pathogen compared to a plant or plant cell ofthe same species lacking the nucleic acid or vector. The term“transgenic plant” refers to a plant comprising such a transgene. A“transgenic plant” includes a plant, plant part, a plant cell or seedwhose genome has been altered by the stable integration of recombinantDNA. A transgenic plant includes a plant regenerated from anoriginally-transformed plant cell and progeny transgenic plants fromlater generations or crosses of a transformed plant. As a result of suchgenomic alteration, the transgenic plant is distinctly different fromthe related wild type plant. An example of a transgenic plant is a plantdescribed herein as comprising one or more of the nucleic acids of thedisclosure, for example encoding Yr5, YrSP or Yr7 proteins or afunctional variant thereof, typically as transgenic elements. Forexample, the transgenic plant includes one or more nucleic acids of thepresent disclosure as transgene, inserted at loci different from thenative locus of the corresponding Yr5, YrSP or Yr7 gene(s). Accordingly,it is herein disclosed a method for producing a transgenic plant,wherein the method comprises the steps of

-   -   (i) transforming a parent plant with no or low resistance to a        fungus,    -   (ii) selecting a plant comprising said one or more nucleic        acid(s) of the invention as transgene(s),    -   (iii) regenerating and    -   (iv) growing said transgenic plant.

In specific embodiments, said transgenic plant is an Angiosperm such asAegilops tauschii, Brachypodium distachyon, Oryza sativa, Triticumturgidum or Triticum aestivum.

For transformation methods within a plant cell, one can cite methods ofdirect transfer of genes such as direct micro-injection into plantembryos, vacuum infiltration or electroporation, direct precipitation bymeans of PEG or the bombardment by gun of particules covered with theplasmidic DNA of interest.

It is preferred to transform the plant cell with a bacterial strain, inparticular Agrobacterium, in particular Agrobacterium tumefaciens. Inparticular, it is possible to use the method described by Ishida et al.(Nature Biotechnology, 14, 745-750, 1996) for the transformation ofmonocotyledons.

Descriptions of Agrobacterium vector systems and methods forAgrobacterium-mediated gene transfer are provided by Moloney et al.,Plant Cell Reports 8:238 (1989). See also, U.S. Pat. No. 5,591,616issued Jan. 7, 1997.

Alternatively, direct gene transfer may be used. A generally applicablemethod of plant transformation is microprojectile-mediatedtransformation wherein DNA is carried on the surface of microprojectilesmeasuring 1 to 4 micron. The expression vector is introduced into planttissues with a biolistic device that accelerates the microprojectiles tospeeds of 300 to 600 m/s which is sufficient to penetrate plant cellwalls and membranes. Sanford et al., Part. Sci. Technol. 5:27 (1987),Sanford, J. C., Trends Biotech. 6:299 (1988), Klein et al.,BioTechnology 6:559-563 (1988), Sanford, J. C., Physiol Plant 7:206(1990), Klein et al., BioTechnology 10:268 (1992). Several targettissues can be bombarded with DNA-coated microprojectiles in order toproduce transgenic plants, including, for example, callus (Type I orType II), immature embryos, and meristematic tissue.

Following transformation of plant target tissues, expression of theselectable marker genes allows for preferential selection of transformedcells, tissues and/or plants, using regeneration and selection methodsnow well known in the art.

The foregoing methods for transformation would typically be used forproducing a transgenic plant including the nucleic acids of theinvention as transgenic element(s).

The transgenic plant could then be crossed, with another(non-transformed or transformed) inbred line, in order to produce a newtransgenic line. Alternatively, a genetic trait which has beenengineered into a particular line using the foregoing transformationtechniques could be moved into another line using traditionalbackcrossing techniques that are well known in the plant breeding arts.For example, a backcrossing approach could be used to move an engineeredtrait from a public, non-elite inbred line into an elite inbred line, orfrom an inbred line containing a foreign gene in its genome into aninbred line or lines which do not contain that gene. As used herein,“crossing” can refer to a simple X by Y cross, or the process ofbackcrossing, depending on the context.

When the term transgenic plant is used in the context of the presentdisclosure, this also includes any plant including, as a transgenicelement one or more of nucleic acids of the invention and wherein one ormore desired traits have further been introduced through backcrossingmethods, whether such trait is a naturally occurring one or a transgenicone. Backcrossing methods can be used with the present invention toimprove or introduce one or more characteristic into the inbred. Theterm backcrossing as used herein refers to the repeated crossing of ahybrid progeny back to one of the parental plants. The parental plantwhich contributes the gene or the genes for the desired characteristicis termed the nonrecurrent or donor parent. This terminology refers tothe fact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental plant to which thegene or genes from the nonrecurrent parent are transferred is known asthe recurrent parent as it is used for several rounds in thebackcrossing protocol (Fehr et al, 1987).

In a typical backcross protocol, the recurrent parent is crossed to asecond nonrecurrent parent that carries the gene or genes of interest tobe transferred. The resulting progeny from this cross are then crossedagain to the recurrent parent and the process is repeated until a plantis obtained wherein all the desired morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant in addition to the gene or genes transferred from the nonrecurrentparent. It should be noted that some, one, two, three or more,self-pollination and growing of a population might be included betweentwo successive backcrosses.

In another aspect the invention relates to a method for producing anon-transgenic plant or plant cell having resistance or enhancedresistance to a fungal pathogen, the method comprising mutating orediting the genomic material of the plant or plant cell to comprise anucleic acid of the invention.

An aspect of the present disclosure relates to a DNA fragment of thecorresponding nucleic acids of the invention (either from naturallyoccurring coding sequence, or improved sequence, such as codon optimizedsequence) combined with genome editing tools (such TALENs, CRISPR-Cas,Cpf1 or zing finger nuclease tools) to target the corresponding Yr5,YrSP or Yr7 genes within the wheat plant genome by insertion at anylocus in the genome or by partial or total allele replacement at thecorresponding locus.

In particular, the disclosure relates to a genetically modified (orengineered) plant, wherein the method comprises the steps of geneticallymodifying a parent plant to obtain in their genome one or more nucleicacids of the invention, preferably by genome-editing, selecting a plantcomprising said one or more one or more nucleic acids as geneticallyengineered elements, regenerating and growing said wheat geneticallyengineered plant.

As used herein, the term “genetically engineered element” refers to anucleic acid sequence present in the genome of a plant and that has beenmodified by mutagenesis or by genome-editing tools, preferentially bygenome-editing tools. In specific embodiments, a genetically engineeredelement refers to a nucleic acid sequence that is not normally presentin a given host genome in the genetic context in which the sequence iscurrently found but is incorporated in the genome of plant by use ofgenome-editing tools. In this respect, the sequence may be native to thehost genome, but be rearranged with respect to other genetic sequenceswithin the host genomic sequence. For example, the geneticallyengineered element is a Yr5, YrSP or Yr7 gene that is rearranged at adifferent locus as compared to a native gene. Alternatively, thesequence is a native coding sequence that has been placed under thecontrol of heterologous regulatory sequences.

In specific embodiments, said genetically engineered plant is anAngiosperm such as Aegilops tauschii, Brachypodium distachyon, Oryzasativa, Triticum turgidum or Triticum aestivum.

The term “genetically engineered plant” or “genetically modified plant”refers to a plant comprising such genetically engineered element. A“genetically engineered plant” includes a plant, plant part, a plantcell or seed whose genome has been altered by the stable integration ofrecombinant DNA. As used herein, the term “genetically engineered plant”further includes a plant, plant part, a plant cell or seed whose genomehas been altered by genome editing techniques. A genetically engineeredplant includes a plant regenerated from an originally-engineered plantcell and progeny of genetically engineered plants from later generationsor crosses of a genetically engineered plant. As a result of suchgenomic alteration, the genetically engineered plant is distinctlydifferent from the related wild type plant. An example of a geneticallyengineered plant is a plant comprising mutated versions of Yr5, YrSP orYr7 encoding genes. In another embodiment, the genetically engineeredplant includes the nucleic acids as genetically engineered elements,inserted at loci different from the native locus of the correspondingYr5, YrSP or Yr7 gene(s).

In specific embodiments, said genetically engineered plants do notinclude plants which could be obtained exclusively by means of anessentially biological process.

Said one or more genetically engineered element(s) enables theexpression of polypeptides which restore or improve resistance tocertain fungus, in particular resistance to a fungal pathogen such aswheat yellow (stripe) rust fungus Puccinia striiformisi f. sp. Tritici,as compared to the parent plant which do not comprise the geneticallyengineered element(s). Typically, said genetically engineered plant is awheat plant, comprising, as the genetically engineered elements, amutated version of Yr5, YrSP or Yr7 encoding gene, and said geneticallyengineered plant has an improved resistance to a fungal pathogen such aswheat yellow (stripe) rust fungus Puccinia striiformisi f. sp. Tritici.

Such genetically engineered plant with improved resistance may bescreened by exposing a variety of genetically engineered plant havingdistinct mutated versions of Yr5, YrSP or Yr7 encoding gene, to a fungalpathogen such as wheat yellow (stripe) rust fungus Puccinia striiformisif. sp. Tritici and selecting the plants which present improvedresistance to said fungal pathogen.

In specific embodiments, a genetically engineered element includes anYr5, YrSP or Yr7 encoding nucleic acid under the control of expressionelements as promoter and/or terminator.

Another aspect of the disclosure relates to a genetically engineeredwheat plant, which comprises the modification by point mutation,insertion or deletion of one or few nucleotides of an Yr5, YrSP or Yr7encoding nucleic acid, as genetically engineered element, into therespectively Yr5, YrSP or Yr7 locus, by any of the genome editing toolsincluding base-editing tool as described in WO2015089406 or bymutagenesis.

The present disclosure further includes methods for improving resistanceto a funal pathogen in a plant by genome editing, comprising providing agenome editing tool capable of replacing partially or totally an Yr5,YrSP or Yr7 encoding nucleic acid or form in a plant by itscorresponding mutated sequence as disclosed herein which confer improvedresistance to said fungal pathogen when expressed in said plant.

Such genome editing tool includes without limitation targeted sequencemodification provided by double-strand break technologies such as, butnot limited to, meganucleases, ZFNs, TALENs (WO2011072246) or CRISPR CASsystem (including CRISPR Cas9, WO2013181440), Cpfl or their nextgenerations based on double-strand break technologies using engineerednucleases.

In another aspect, the invention relates to a plant or plant cellobtained or obtainable by a method of the invention. The plant or plantcell may be a crop plant or plant cell or a biofuel plant or plant cell,for example selected from maize, wheat, tobacco, oilseed rape, sorghum,soybean, potato, tomato, grape, barley, pea, bean, field bean, lettuce,cotton, sugar cane, sugar beet, broccoli or other vegetable brassicas orpoplar.

In another aspect, the invention relates to a seed of the plant of theinvention wherein the seed comprises a nucleic acid or an NLRpolypeptide of the invention. The seed may be a wheat seed.

In another aspect, the invention relates to a method of limiting wheatyellow (stripe) rust in agricultural crop production, the methodcomprising planting a wheat seed as according to the invention andgrowing a wheat plant under conditions favourable for the growth anddevelopment of the wheat plant.

In another aspect, the invention relates to a method for identificationor selection of an organism such as plant having resistance to a fungussuch as wheat yellow (stripe) rust fungus Puccinia striiformisi f. sp.tritici, comprising the step of screening the organism for the presenceor absence of: (1) a nucleic acid as defined according to the invention;and/or (2) an NLR polypeptide according to the invention, whereinpresence of the nucleic acid or the NLR polypeptide indicatesresistance.

Accordingly, it is disclosed herein the means for specifically detectingthe nucleic acids of the present invention in a wheat plant.

Such means include for example a pair of primers for the specificamplification of a fragment nucleotide sequence specific of the nucleicacids of the invention in the plant genomic DNA.

As used herein, a primer encompasses any nucleic acid that is capable ofpriming the synthesis of a nascent nucleic acid in a template-dependentprocess, such as PCR. Typically, primers are oligonucleotides from 10 to30 nucleotides, but longer sequences can be employed. Primers may beprovided in double-stranded form though single-stranded form ispreferred.

Alternatively, nucleic acid probe can be used for the specific detectionof any one of the nucleic acids.

As used herein, a nucleic acid probe encompass any nucleic acid of atleast 30 nucleotides and which can specifically hybridizes understandard stringent conditions with a defined nucleic acid. Standardstringent conditions as used herein refers to conditions forhybridization described for example in Sambrook et al 1989 which cancomprise 1) immobilizing plant genomic DNA fragments or library DNA on afilter 2) prehybridizing the filter for 1 to 2 hours at 65° C. in 6× SSC5× Denhardt's reagent, 0.5% SDS and 20 mg/ml denatured carrier DNA 3)adding the probe (labeled) 4) incubating for 16 to 24 hours 5) washingthe filter once for 30 min at 68° C. in 6× SSC, 0.1% SDS 6) washing thefilter three times (two times for 30 min in 30 ml and once for 10 min in500 ml) at 68° C. in 2× SSC 0.1% SDS. The nucleic acid probe may furthercomprise labeling agent, such as fluorescent agents covalently attachedto the nucleic acid part of the probe.

In certain embodiments, said nucleic acid probe is a fragment of atleast 20 bp, 30 bp, 40 bp, 50 bp, 60 bp, 70 bp, 80 bp, 90 bp, 100 bp,110 bp, 120 bp, 130 bp, 140 bp, 150 bp, 160 bp or the whole fragment ofany of SEQ ID NO:4, 5 or 7.

References to “variant” include a genetic variation in the native,non-mutant or wild type sequence. Examples of such genetic variationsinclude mutations selected from: substitutions, deletions, insertionsand the like.

More generally, as used herein the term “polypeptide” refers to apolymer of amino acids. The term does not refer to a specific length ofthe polymer, so peptides, oligopeptides and proteins are included withinthe definition of polypeptide. The term “polypeptide” may includepolypeptides with post-expression modifications, for example,glycosylations, acetylations, phosphorylations and the like. Includedwithin the definition of “polypeptide” are, for example, polypeptidescontaining one or more analogs of an amino acid (including, for example,unnatural amino acids), polypeptides with substituted linkages, as wellas other modifications known in the art both naturally occurring andnon-naturally occurring.

As used herein, a “functional variant or homologue” is defined as apolypeptide or nucleotide with at least 50% sequence identity, forexample at least 55% sequence identity, at least 60% sequence identity,at least 65% sequence identity, at least 70% sequence identity, at least75% sequence identity, at least 80% sequence identity, at least 85%sequence identity, at least 90% sequence identity, at least 95% sequenceidentity, at least 96% sequence identity, at least 97% sequenceidentity, at least 98% sequence identity, or at least 99% sequenceidentity with the reference sequence.

Sequence identity between nucleotide or amino acid sequences can bedetermined by comparing an alignment of the sequences. When anequivalent position in the compared sequences is occupied by the samebase or amino acid, then the molecules are identical at that position.Scoring an alignment as a percentage of identity is a function of thenumber of identical amino acids or bases at positions shared by thecompared sequences. When comparing sequences, optimal alignments mayrequire gaps to be introduced into one or more of the sequences to takeinto consideration possible insertions and deletions in the sequences.Sequence comparison methods may employ gap penalties so that, for thesame number of identical molecules in sequences being compared, asequence alignment with as few gaps as possible, reflecting higherrelatedness between the two compared sequences, will achieve a higherscore than one with many gaps. Calculation of maximum percent identityinvolves the production of an optimal alignment, taking intoconsideration gap penalties.

Suitable computer programs for carrying out sequence comparisons arewidely available in the commercial and public sector. Examples includeMatGat (Campanella et al., 2003, BMC Bioinformatics 4: 29; programavailable from http://bitincka.com/ledion/matgat), Gap (Needleman &Wunsch, 1970, J. Mol. Biol. 48: 443-453), FASTA (Altschul et al., 1990,J. Mol. Biol. 215: 403-410; program available fromhttp://www.ebi.ac.uk/fasta), Clustal W 2.0 and X 2.0 (Larkin et al.,2007, Bioinformatics 23: 2947-2948; program available fromhttp://www.ebi.ac.uk/tools/clustalw2) and EMBOSS Pairwise AlignmentAlgorithms (Needleman & Wunsch, 1970, supra; Kruskal, 1983, In: Timewarps, string edits and macromolecules: the theory and practice ofsequence comparison, Sankoff & Kruskal (eds), pp 1-44, Addison Wesley;programs available from http://www.ebi.ac.uk/tools/emboss/align). Allprograms may be run using default parameters.

For example, sequence comparisons may be undertaken using the “Needle”method of the EMBOSS Pairwise Alignment Algorithms, which determines anoptimum alignment (including gaps) of two sequences when considered overtheir entire length and provides a percentage identity score. Defaultparameters for amino acid sequence comparisons (“Protein Molecule”option) may be Gap Extend penalty: 0.5, Gap Open penalty: 10.0, Matrix:Blosum 62. Default parameters for nucleotide sequence comparisons (“DNAMolecule” option) may be Gap Extend penalty: 0.5, Gap Open penalty:10.0, Matrix: DNAfull.

In one aspect of the invention, the sequence comparison may be performedover the full length of the reference sequence.

Particular non-limiting embodiments of the present invention will now bedescribed in detail.

EXAMPLES Example 1

Introduction

Here we isolate and characterise three major yellow rust resistancegenes (Yr7, Yr5, and YrSP) effective in hexaploid wheat (Triticumaestivum), each having a distinct and unique recognition specificity. Weshow that Yr5, which remains effective to a broad range of PST isolatesworldwide, is allelic to YrSP and paralogous to Yr7, both of which havebeen overcome by multiple PST isolates. All three Yr genes belong to acomplex gene cluster on chromosome 2B encoding nucleotide-binding andleucine-rich repeat proteins (NLRs) with a non-canonical N-terminalzinc-finger BED domain that is distinct from those found in non-NLRwheat proteins. We developed and tested diagnostic markers to acceleratehaplotype analysis and marker-assisted selection for breeding, enablingstacking of the non-allelic Yr genes. Our results provide evidence thatthe BED-NLR gene architecture can provide effective field-basedresistance to important fungal diseases such as wheat yellow rust.

Results and Discussion

To clone the genes encoding Yr5, Yr7 and YrSp, we identified ethylmethanesulfonate-derived susceptible mutants from different geneticbackgrounds carrying these genes (FIG. 1, Tables 2-3). We performedMutRenSeq (see Methods) and identified a single candidate contig foreach of the three genes based on nine, ten, and four independentsusceptible mutants, respectively (FIG. 1A and FIG. 4). The threecandidate contigs were genetically linked to a common mapping intervalpreviously identified for the three Yr loci. Additionally, their closesthomologs in the Chinese Spring wheat genome sequence (RefSeq,https://wheat-urgi.versailles.inra.fr/Seq-Repository/Assemblies) liebetween the flanking markers defining the genetic mapping interval (FIG.1B and 5). Within each contig we predicted a single open reading framebased on RNA-Seq data. All three predicted Yr genes displayed similarexon-intron structures (FIG. 1A), although YrSP was truncated in exon 3due to a single bp deletion that results in a premature terminationcodon. The DNA sequences of Yr7 and Yr5 were 77.9% identical across thecomplete gene, whereas YrSP was a truncated version of Yr5, sharing99.8% identity in the common sequence. This suggests that Yr5 and YrSPare encoded by alleles of the same gene, but are paralogous to Yr7. The23 mutations identified by MutRenSeq were confirmed by Sanger sequencingand lead to either an amino acid substitution or a truncation allele(splice junction or termination codon)(FIG. 1A, Table 3). Takentogether, the mutant and genetic analyses demonstrate that these twogenes encode for Yr7 and Yr5/YrSP.

The Yr7, Yr5 and YrSP proteins contain a zinc-finger BED domain at theN-terminus, followed by the canonical NB-ARC domain. Only Yr7 and Yr5proteins encode multiple LRR motifs at the C-terminus. YrSP lost most ofthe LRR region due to the presence of a premature termination codon inexon 3 (FIG. 2A). However, YrSP still confers functional resistance toPST, although having a different recognition specificity to Yr5. Yr7 andYr5/YrSP are highly conserved in the N-terminus, with a singleamino-acid change in the BED domain, but this high degree ofconservation is eroded after the BED domain (FIG. 2A). The BED domain isrequired for Yr7-mediated resistance, as a single amino acid change inthe mutant line Cad0903 led to a susceptible reaction (FIG. 1A).However, recognition specificity is not solely governed by the BEDdomain, as the Yr5 and YrSp alleles have identical BED domain sequencesand yet confer resistance to different PST isolates.

We examined the allelic variation in Yr7 and Yr5/YrSP across eightsequenced tetraploid and hexaploid wheat genomes (Table 4). Yr7 wasoriginally derived from tetraploid durum wheat (T. turgidum ssp. durum)cultivar Iumillo and was spread globally through hexaploid cultivarThatcher. We identified Yr7 only in Cadenza (Thatcher-derived) andParagon, which is identical by descent to Cadenza in this interval(Table 5a and b). None of the three sequenced tetraploid accessions(Svevo, Kronos, Zavitan) carried Yr7.

For Yr5/YrSP, we identified three additional alleles in the sequencedhexaploid wheat cultivars (Table 5a and b). Claire encodes a completeNLR with only six amino-acid changes situated outside the threeconserved domains (BED, NB-ARC and LRRs) and six polymorphisms in theC-terminus compared to Yr5. Robigus, Paragon and Cadenza also encode afull length NLR which shares common polymorphisms with Claire inaddition to 19 amino acid substitutions across the BED and NB-ARCdomains. Tetraploid Kronos and Svevo encode a fifth Yr5/YrSP proteinwith a truncation in the LRR region distinct from YrSP, in addition tomultiple amino acid substitutions in the C-terminus. This truncatedtetraploid allele is reminiscent of YrSP and is expressed in Kronos (seeMethods). None of these varieties exhibit a typical Yr5 resistanceresponse, suggesting that these amino acid changes/truncations may alterrecognition specificity or protein function.

We designed diagnostic markers for Yr5 and Yr7 to facilitate theirdetection and use in breeding. We confirmed their presence in the donorcultvars Thatcher and Lee (Yr7), Spaldings Prolilic (YrSP), and speltwheat cv. Album (Yr5) (Tables 10-12; FIGS. 10 and 12). To further definetheir specificity, we tested the markers in a collection of globallandraces and European varieties released over the past one hundredyears. Yr5 was only present in spelt cv. Album, AvocetS-Yr5, andLemhi-Yr5 and was not detected in any other line (Table 19), consistentwith the fact that Yr5 has not yet been deployed within Europeanbreeding programmes. Yr7 on the otherhand was more prevalent in thegermplasm tested and we could track its presence across pedigreesincluding Cadenza derived cultivars (see Tables 11-15; FIG. 10).

We defined the Yr7/Yr5/YrSP syntenic interval across the wheat genomesand related grass species Aegilops tauschii (D genome progenitor),Hordeum vulgare (barley), Brachypodium distachyon and Oryza sativa(rice) (FIG. 6). We identified both canonical NLRs as well as integratedBED-NLRs across all genomes and species, except for barley, whichcontained only canonical NLRs across the syntenic region. Thephylogenetic relationship based on the NB-ARC domain suggests a commonevolutionary origin of these integrated domain NLR proteins before thewheat-rice divergence (50 Mya) and an expansion in the number of NLRs inthe A and B genomes of polyploid wheat species (FIG. 7, FIG. 3A). Withinthe interval we also identified several genes in the A, B and D genomesthat encode two consecutive in-frame BED domains in frame (herein namedBED_I and BED_II) followed by the canonical NLR. These double BED domaingenes had each BED domain fully encoded within a single exon (exons 2and 3) and in most cases had a four-exon structure (FIG. 3B). This isconsistent with the three exon structure of single BED domain genes,such as Yr7 and Yr5/YrSP (BED_I type encoded on exon 2). Very few aminoacids were conserved between BED_I and II (FIG. 3B). To our knowledgethis is the first report of the double BED domain NLR protein structureto date. The biological function of this molecular innovation remains tobe determined, although our data show that the single BED_I structurecan confer PST resistance and is required for Yr7-mediated resistance.

Among other mechanisms, integrated domains of NLRs are hypothesised toact as decoys for their intended effector targets. This would suggestthat the integrated domain might be sequence-related to the host proteintargeted by the effector. To identify potential host targets of AvrYr7,AvrYr5 and AvrYrSP, we retrieved all BED-domain proteins (108) from thewheat genome, including 25 BED-NLRs, and additional BED-NLRs located inthe syntenic intervals (Table 6). We also retrieved the rice Xal andZBED proteins, the latter being hypothesized to act in rice resistanceagainst Magnaporthe. oryzae. We used the split network methodimplemented in Splitstree4 to represent the relationships between theseBED domains (FIG. 3C, FIG. 8). We found a major split in the network,with almost all wheat non-NLR BED proteins (76 of 83) clusteringtogether at one end and the BED-NLRs proteins of wheat and otheranalysed species at the other end. This clear separation is consistentwith the hypothesis that integrated domains might have evolved tostrengthen the interaction with the effector after integration. AmongBED-NLRs, BED_I and BED_II constitute two major clades that arecomprised solely of genes from within the Yr7/Yr5/YrSP syntenic region.The seven non-NLR BED domain wheat proteins that clustered with BED-NLRsare most closely related to the Brachypodium and rice proteins and werenot expressed in RNA-Seq data from a Yr5-mediated resistance vssusceptible time-course (FIG. 9, Table 12). Similarly, no BED-containingprotein was differentially expressed during this infection time-course.This is consistent with the prediction that effectors alter theirtargets' activity at the protein level. However, we cannot disprove thatthese closely related BED-containing proteins are involved inBED-NLRs-mediated resistance.

BED-NLRs are frequent in Triticeae and occur in other monocot and dicottribes. However, only a single BED-NLR gene, Xa1, had been previouslyshown to confer resistance to plant pathogens. In the present study, weshow that the distinct Yr5, YrSP, and Yr7 resistance specificitiesbelong to a complex NLR cluster on chromosome 2B and are encoded by twoBED-NLRs genes which are paralogous. We report an allelic series for theYr5/YrSP gene with five independent alleles including three full-lengthBED-NLRs (including Yr5) and two truncated versions (including YrSP).This wider allelic series could be of functional significance aspreviously shown for the Mla and Pm3 loci that confer resistance toBlumeria graminis in barley and wheat, respectively, and the flax Llocus conferring resistance to Melampsora lini. Overall, our results addstrong evidence for the importance of the BED-NLR architecture inplant-pathogen interactions. The paralogous and allelic relationship ofthese three distinct Yr loci will inform future hypothesis-drivenengineering of novel recognition specificities.

Methods

1.1. MutRenSeq

Mutant Identification

Table 2 summarises plant materials and PST isolates used for each Yrgene. We used an ethyl methanesulfonate (EMS)-mutagenised population incultivar Cadenza to identify mutants in Yr7, whereas EMS-populations inthe corresponding AvocetS-Yr near isogenic line (NIL) were used toidentify Yr5 and YrSP mutants. For Yr7, we inoculated M₃ plants from theCadenza EMS population with PST isolate 08/21 which is virulent to Yr1,Yr2, Yr3, Yr4, Yr6, Yr9, Yr17, Yr27, Yr32, YrRob, and YrSol. Wehypothesised that susceptible mutants would carry mutations in Yr7.Plants were grown in 192-well trays in a confined glasshouse with nosupplementary lights or heat. Inoculations were performed at the oneleaf stage (Z11) with a talc-urediniospore mixture. Trays were kept indarkness at 10° C. and 100% humidity for 24 hours. Infection types (IT)were recorded 21 days post-inoculation following the Grassner and Straibscale. Identified susceptible lines were progeny tested to confirm thereliability of the phenotype and DNA from M₄ plants was used for RenSeq(see section below). Similar methods were used for AvocetS+Yr7,AvocetS+Yr5 and AvocetS+YrSp EMS-mutagenised populations with thefollowing exceptions: PST pathotypes 108 E141 A+ (University of SydneyPlant Breeding Institute Culture no. 420),150 E16 A+(Culture no. 598)and 134 E16 A+(Culture no. 572) were used, respectively. EMS-derivedsusceptible mutants in Lehmi+Yr5 were previously identified and DNA fromM₅ plants was used for RenSeq.

DNA Preparation and Resistance Gene Enrichment and Sequencing (RenSeq)

We extracted total genomic DNA from young leaf tissue using thelarge-scale DNA extraction protocol from the McCouch Rice Lab(https://ricelab.plbr.cornell.edu/dna_extraction). Total genomic DNA ofall Avocet mutants and wild-types were extracted following a previouslydescribed method. We checked DNA quality and quantity on a 0.8% agarosegel and with a NanoDrop spectrophotometer (Thermo Scientific). ArborBiosciences (Ann Arbor, Mich., USA) performed the targeted enrichment ofNLRs according to the MYbaits protocol and using an improved version ofthe Triticeae bait library. Library construction was performed using theTruSeq RNA protocol v2 (Illumina 15026495). Libraries were pooled—onepool of samples for Cadenza mutants and one of eight samples for theLemhi+Yr5 parent and Lemhi+Yr5 mutants. AvocetS+Yr5 and AvocetS+YrSPwild type together with their respective mutants were also processedaccording to the aforementioned MYbaits protocol and the same baitlibrary were used. All enriched libraries were sequenced on a HiSeq 2500(Illumina) in High Output mode using 250 bp paired end reads and SBSchemistry. We used Cadenza wild-type data previously generated on anIllumina MiSeq instrument.

In addition to the mutants, we also generated RenSeq data for Kronos andParagon to confirm the presence of the Yr5 allele in Kronos and the Yr7gene in Paragon

Details of all the lines sequenced is available in Table 3 andsequencing details are in Table 8.

1.2. MutantHunter Pipeline

We adapted the pipeline from https://github.com/steuernb/MutantHunter/toidentify candidate contigs for the targeted Yr genes. First, we trimmedthe RenSeq-derived reads with trimmomatic and the following parameters:ILLUMINACLIP:TruSeq2-PE.fa:2:30:10 LEADING:30 TRAILING:30SLIDINGWINDOW:10:20 MINLEN:50 (v0.33). We made de novo assemblies ofwild-type plant trimmed reads with the CLC assembly cell and defaultparameters apart from the word size (-w) parameter that we set to 64(v5.0, http://www.cicbio.com/products/c1c-assembly-cell!, Table 9). Wethen followed the MutantHunter pipeline detailed athttps://github.com/steuernb/MutantHunter/. For Cadenza mutants, we usedthe following MutantHunter program parameters to identify candidatecontigs: -c 20-n 6-z 1000, that translates into SNPs with at least 20xcoverage, six susceptible mutants must have a mutation in the contig toreport it as candidate, and small deletions were filtered out by settingthe number of coherent positions with zero coverage to call a deletionmutant at 1000. The -n parameter was modified accordingly in subsequentruns with the Lemhi+Yr5 (−n 6). For identifying Yr5 and YrSP contigsfrom Avocet mutants, we followed the aforementioned MutantHunter withall default parameters, except the use of CLC Genomics Workbench (v10)for reads QC and trimming, as well as de novo assemblies of Avocetwild-type and mapping all reads against de novo assembly of wild-type.The MutantHunter programme parameters were set all as default except for−z was set as 100. The parameter −n was set for two as the first run andthen three as the second run. Regarding Yr5, two mutants were siblinglines as they carried the same mutation at identical positions (FIG. 4,Table 3).

For Yr7 we identified a single contig with six mutations, however we didnot identify mutations in line Cad0903. Upon examination of the Yr7candidate contig we predicted that the 5′ region was likely missing(FIG. 4). We thus annotated potential NLRs in the Cadenza genomeassembly available from the Earlham Institute (Table 4,http://opendata.earlham.ac.uk/Triticum aestivum/EI/v1.1) with theNLR-Annotator program with standard parameters(https://github.com/steuernb/NLR-Annotator). We identified an annotatedNLR in the Cadenza genome with 100% sequence identity to the Yr7candidate contig, but that extended beyond the available sequence. Wetherefore replaced the previous candidate contig with the extendedCadenza sequence (100% sequence identity) and mapped the RenSeq readsfrom the Cadenza wild-type and mutants the same way as above. Thisconfirmed the candidate for Yr7 as we retrieved the missing 5′ regionincluding the BED domain, and confirmed a mutation in the outstandingmutant line Cad0903 (FIG. 4).

The Triticeae bait library does not include integrated domains in itsdesign so they are prone to be missed, especially when located at theends of an NLR. Sequencing technology could also have accounted forthis: MiSeq was used for Cadenza wild-type whereas HiSeq was chosen forLemhi-Yr5 and we did not observe the missing 5′ region in the latter,although coverage was lower than the regions encoding for canonicaldomains.

In summary, we sequenced nine, ten and four mutants for Yr7, Yr5 andYrSP and identified a single contig for each target gene which accountedfor all the mutations.

1.3. Candidate Contig Confirmation and Gene Annotation

We sequenced the three candidate contigs to confirm the EMS-derivedmutations using primers documented in Table 10. We first PCR-amplifiedthe full locus from the same DNA preparations as the ones submitted forRenSeq with the Phusion® High-Fidelity DNA Polymerase (New EnglandBiolabs) following the provider's protocol(https://www.neb.com/protocols/0001/01/01/per-protocol-m0530). We thencarried out nested PCR on the obtained product to generate overlapping600-1,000 bp amplicons that were purified using the MiniElute kit(Qiagen). The purified PCR products were sequenced by GATC following theLightRun protocol(https://www.gatc-biotech.com/shop/en/lightrun-tube-barcode.html).Resulting sequences were aligned to the wild-type contig usingClustalOmega (https://www.ebi.ac.uk/Tools/msa/clustalo/). This allowedus to curate the Yr7 locus in the Cadenza assembly that has two ‘N’ inits sequence, corresponding to a 39 bp insertion and a 129 bp deletion,and confirm the presence of the mutations in each mutant line.

We used HISATt2 (v2.1) to map RNA-Seq reads available from Cadenza andAvocetS-Yr5 onto the RenSeq de novo assemblies with curated loci todefine the gene structure of the genes. We used the followingparameters: —no-mixed—no-discordant to map read in pairs only. We usedthe—novel-splicesite-outfile to predict splicing sites which we manuallychecked with the genome visualisation tool IGV (v2.3.79). Predicted CDSwere then translated using the ExPASy online tool(https://web.expasy.org/translate/). This allowed us to predict theeffect of the mutations for each candidate gene (FIG. 1A). Thelong-range primers for both Yr7 and Yr5 loci were then used on thecorresponding susceptible Avocet NIL mutants to determine whether thegenes were present and carried mutations in that background (FIG. 1A).

1.4. Genetic Linkage Experiments

We generated a set of F₂ populations to genetically map the candidatecontigs (Table 2). For Yr7 we developed an F₂ population based a crossbetween the susceptible mutant line Cad0127 to the Cadenza wild typecontrol (population size 139 individuals). For Yr5 and YrSp we developedF₂ populations between AvocetS and the NILs carrying the correspondingYr gene (94 individuals for YrSp and 376 for Yr5). We extracted DNA fromleaf tissue at the seedling stage (Z11). Rqtl package was used toproduce the genetic map based on a general likelihood ratio test andgenetic distances were calculated from recombination frequencies(v1.41-6).

We used markers linked to Yr7, Yr5, YrSP (WMS526, WMS501 and WMC175,WMC332, respectively) in addition to closely linked markers WMS120,WMS191 and WMC360 (based on the GrainGenes databasehttps://wheat.pw.usda.gov/GG3/) to define the physical region on RefSeqv1.0. Two different approaches were used for genetic mapping dependingon the material. For Yr7, we used the public data for Cad0127(www.wheat-tilling.com) to identify nine mutations located within theYr7 physical interval based on BLAST analysis against RefSeq v1.0. Weused KASP primers when available and manually designed additional onesincluding an assay targeting the Cad0127 mutation in the Yr7 candidatecontig (Table 10). We genotyped the Cad0127 F₂ populations using theseten KASP assays and confirmed genetic linkage between the Cad0127 Yr7candidate mutation and the nine mutations across the physical interval(FIG. 5).

For Yr5 and YrSP, we first aligned the candidate contigs to the bestBLAST hit in an AvocetS RenSeq de novo assembly. We then designed KASPprimers targeting polymorphism between these sequences and used them togenotype the corresponding F₂ population. We also used markerspolymorphic between parental lines to determine the presence of Yr5/YrSPin breeding material (Table 10). For both candidate contigs we confirmedgenetic linkage with the genetic intervals for these Yr genes (FIG. 5).

1.5. Yr7 Gene-Specific Markers

We aligned the Yr7 sequence with the best BLAST hits in the genomeslisted on Table 2 and designed KASP primers targeting polymorphisms thatwere Yr7-specific. Three markers were retained after testing on aselected panel of Cadenza-derivatives and varieties that were positivefor Yr7 markers in the literature, including the Yr7 reference cultivarLee (Table 10 for the primers, Tables 11 and 12 for the results). Thepanel of Cadenza-derivatives was phenotyped with three PST isolates: PST08/21 (Yr7-avirulent), PST 15/151 (Yr7-avirulent—virulent toYr1,2,3,4,6,9,17,25,32,Rendezvous, Sp, Robigus, Solstice) and PST 14/106(Yr7-virulent, virulent to Yr1,2,3,4,6,7,9,17,25,32, Sp, Robigus,Solstice, Warrior, Ambition, Cadenza, KWS Sterling, Apache) to determinewhether Yr7-positive varieties as determined by the three KASP markersdisplayed a consistent specificity. Pathology assays were performed asfor the screening of the Cadenza mutant population. We retrievedpedigree information for the analysed varieties from the GeneticResources Information System for Wheat and Triticale database (GRIS,www.wheatpedigree.net) and used the Helium software (v1.17) toillustrate the breeding history of Yr7 in the UK (FIG. 10).

We used the three Yr7 KASP markers to genotype (i) varieties from theAHDB Wheat Recommended List from 2005-2018(https://cereals.andb.org.uk/varieties/andb-recommended-lists.aspx);(ii) the Gediflux collection that gathers European bread wheat varietiesreleased between 1920 and 2010 and (iii) the core Watkins collection,which represents a global set of wheat landraces collected in the 1930s.Results are reported in Tables 13-15.

Yr5 Gene-Specific Markers

We identified a 774 bp insertion in the Yr5 allele 29 bp upstream theSTOP codon with respect to the Cadenza and Claire alleles. gDNA fromYrSP confirmed that the insertion was specific to Yr5.

We used this polymorphism to design primers flanking the insertion andtested them on a subset of the collections mentioned above. We includedDNA from Triticum aestivum ssp. spelta var. Album (Yr5 donor) andSpaldings Prolific (YrSP donor) to assess their amplification profiles.PCR amplification was conducted using a touchdown programme with thefirst 10 cycles from 67° C. to 62° C. (−0.5° C. per cycle) and theremaining 25 cycles at 62° C. This allowed to increase the specificityof the reaction. We observed three different profiles on the testedvarieties (i)1,281 bp amplicon in Yr5 positive cultivars, (ii) 507 bpamplicon in the alternate Yr5 alleles carriers including YrSP, Cadenzaand Claire and (iii) no amplification in other varieties. We sequencedthe different amplicons and confirmed the insertion in Yr5 compared tothe alternate alleles. The lack of amplicon in some varieties mightrespresent the absence of the loci in the tested varieties.

1.6. In Silico Allele Mining for Yr7 and Yr5

We used the Yr7 and Yr5 sequences to retrieve the best BLAST hits in theT. aestivum and T. turgdium wheat genomes listed in Table 4. The bestYr5 hits shared between 93.6 and 99.3% sequence identity, which wascomparable to what was observed for alleles derived from the barley Pm3(>97% identity) and flax L (>90% identity) genes. Yr7 was identifiedonly in Paragon and Cadenza (Table 5a and b; see FIG. 11 for curation ofthe Paragon sequence).

1.7. Analysis of the Yr7 and Yr5/YrSP Cluster on RefSeq v1.0

Definition of Syntenic Regions Across Grass Genomes

We used NLR-Annotator to identify putative NLR loci on RefSeq v1.0chromosome 2B and identified the best BLAST hits to Yr7 and Yr5 onRefSeq v1.0. Additional BED-NLRs and canonical NLRs were annotated inclose physical proximity to these best BLAST hits. Therefore, to betterdefine the NLR cluster we selected ten non-NLR genes located both distaland proximal to the region and identified orthologs in barley,Brachypodium and rice in EnsemblPlants (https://plants.ensembl.org/). Weused different % ID cutoffs for each species (>92% for barley, >84% forBrachypodium and >76% for rice) and determined the syntenic region whenat least three consecutive orthologues were found. A similar approachwas conducted for Triticum ssp and Ae. tauschii (Table 16).

1.8. Definition of the NLR Content of the Syntenic Region

We extracted the previously defined syntenic region from the grassgenomes listed in Table 4 and annotated NLR loci with NLR-Annotator. Wemaintained previously defined gene models where possible, but alsodefined new gene models which were further analysed through a BLASTxanalysis to confirm the NLR domains (Tables 16-18). The presence of BEDdomains in these NLRs was also confirmed by CD-Search(https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi). All NLR andBED-NLR encoding sequences were taken forward for reciprocal BLASTanalyses across all genomes to identify orthologous relationships. NLRsare known to be more variable than other gene classes so we used a lowerthreshold to define orthologues (80% ID across 80% of the alignment forthe Triticeae (brown lines on FIG. 6)).

1.9. Phylogenetic and Neighbour Network Analyses

We aligned the translated NB-ARC domains from the NLR-Annotator outputwith MUSCLE and standard parameters (v.3.8.31). We verified and manuallycurated the alignment with Jalview (v2.10.1). We built a MaximumLikelihood tree with the RAxML program and the following parameters:raxmlHPC -f a -x 12345-p 12345-N 1000-m PROTCATJTT -s<input_alignmentlasta>(MPI version v8.2.10). The best scoring tree withassociated bootstrap values was visualised with Dendroscope (v3.5.9).

We used the Neighbour-net method implemented in SplitsTree4 to analyserelationships between BED domains from NLR and non-NLR proteins (v4.16).We first retrieved all BED-containing proteins from RefSeq v1.0 asfollows: we used hmmer (v3.1b2, http://hmmer.org/) to identify conserveddomain in protein sequences from RefSeq v1.0. We applied a cut-off of0.01 on i-evalue to filter-off any irrelevant identified domains. Weseparated the set between NLR and non-NLRs based on the presence of theNB-ARC and sequence homology for single BED proteins. BED domains wereextracted from the corresponding protein sequences based on the hmmeroutput and were verified on the CD-search database. Alignments of theBED domains were performed the same way as for NB-ARC domains and wereused to generate a neighbour network in SplitsTree4 based on theuncorrected P distance matrix.

1.10. Transcriptome Analysis

Kronos Analysis

We reanalysed RNA-Seq from cultivar Kronos to determine whether theKronos Yr5 alelle was expressed. We followed the same strategy as thatdescribed to define the Yr7 and Yr5 gene structure (candidate contigconfirmation and gene annotation section). We generated a de novoassembly of the Kronos NLR repertoire from Kronos RenSeq data and usedit as a reference to map read data of one replicate from the wild-typeKronos heading stage. Read depths up to 30× were present in the Yr5allele which allowed to confirm its expression. Likewise, the RNA-Seqreads confirmed the gene structure, which is similar to YrSP, and thepremature termination codon in Kronos Yr5.

Re-Analysis of RNAseq Data in Dobon et al., 2016

Briefly, two RNA-Seq time-courses were used based on samples taken fromleaves at 0, 1, 2, 3, 5, 7, 9 and 11 days post-inoculation for thesusceptible cultivar Vuka and 0, 1, 2, 3 and 5 days post inoculation forthe resistant AvocetS-Yr5. We used normalised read counts (TranscriptPer Million, TPM) from Ramirez-Gonzalez et al. (2018; under review) toproduce the heatmap shown in FIG. 11 with the pheatmap R package(v1.0.8). Transcripts were clustered according to expression profiledefined by a Euclidean distance matrix and hierarchical clustering.Transcripts were considered expressed if their average TPM was 0.5 TPMin at least one time point. We used the DESeq2 R package (v1.18.1) toconduct a differential expression analysis. We performed twocomparisons: (1) we used a likelihood ratio test to compare the fullmodel ˜Variety +Time +Variety:Time to the reduced model ˜Variety +Timeto identify genes that were differentially expressed between the twovarieties at a given time point after time 0 (workflow:https://www.bioconductor.org/help/workflows/rnaseqGene/); (2)Investigation of both time courses in Vuka and AvocetS-Yr5 independentlyto generate all of the comparisons between time 0 and a given timepoint, following the standard DESeq2 pipeline. Differentially expressedgenes were considered to be those with an adjusted p-value <0.05 and alog2 fold change of 2 or higher.

Although the present invention has been described with reference topreferred or exemplary embodiments, those skilled in the art willrecognize that various modifications and variations to the same can beaccomplished without departing from the spirit and scope of the presentinvention and that such modifications are clearly contemplated herein.No limitation with respect to the specific embodiments disclosed hereinand set forth in the appended claims is intended nor should any beinferred.

All documents cited herein are incorporated by reference in theirentirety.

TABLE 1 Summary of the data from NIABTAG Seedstats journal (NIABTAGNetwork) and UK Cereal Pathogen Virulence Survey(http://www.niab.com/pages/id/316/UKCPVS) that were used Table 1: CerealWeights Certified-NIAB TAG for selected Yr7 varieties from 1990 to 2016with virYr7 prevalence among UK yellow rust isolates (UKCPVS) CultivatedYr7 varieties 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 %virYr7_isolat

9 19 7 8 4 0 3 7 4 10 CORDIALE total tons 0 0 0 0 0 0 0 0 0 0 % 0.0 0.00.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 CUBANITA total tons 0 0 0 0 0 0 0 0 0 0% 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 GRAFTON total tons 0 0 0 0 0 00 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 SKYFALL total tons 0 00 0 0 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 RUSKIN totaltons 0 0 0 0 0 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 BROCKtotal tons 3666.8 934.4 389 127.3 80.7 0 0 0 0 0 % 1.3 0.3 0.2 0.0 0.00.0 0.0 0.0 0.0 0.0 CADENZA total tons 0 0 337.5 8011.3 8412.3 3345.31146.4 634.5 744.8 223.5 % 0.0 0.0 0.1 3.1 3.4 1.3 0.4 0.3 0.3 0.1 CAMPtotal tons 1450.35 462.7 217 215.9 81.7 56.8 31.2 0 0 0 REMY % 0.5 0.20.1 0.1 0.0 0.0 0.0 0.0 0.0 0.0 PROPHET total tons 0 0 0 124.2 29 0 0 00 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 SOLEIL total tons 65 47.7152.5 71.5 60 15 0 0 0 0 % 0.0 0.0 0.1 0.0 0.0 0.0 0.0 0.0 0.0 0.0 SPARKtotal tons 0 0 2402.7 3734.2 3240.6 2737.9 2369.6 1627.1 1036.9 809.3 %0.0 0.0 1.0 1.5 1.3 1.0 0.9 0.7 0.5 0.4 TARA total tons 392.3 3018.7 74885.7 49.6 0 0 0 0 0 % 0.1 1.1 0.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 total282286 283787 240546 255647 245240 261883 270400 247852 229351 222203varieties total % 2.0 1.6 1.8 4.8 4.9 2.4 1.3 0.9 0.8 0.5 Yr7 CultivatedYr7 varieties 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 %virYr7_isolat

4 0 3 36 4 8 11 4 0 0 total tons 0 0 21 969 5307 4819 6466 8013 1076412346 % 0.0 0.0 0.0 0.5 2.9 3.1 4.3 4.3 5.7 7.1 total tons 0 0 0 0 0 0 00 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 total tons 0 0 0 0 0 0 00 191 5010 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 2.9 total tons 0 0 0 00 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 total tons 0 0 0 00 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 total tons 0 0 0 00 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 total tons 234.8132.65 117 60 39 0 0 0 0 0 % 0.1 0.1 0.1 0.0 0.0 0.0 0.0 0.0 0.0 0.0total tons 0 0 0 0 0 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0total tons 0 0 0 0 0 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0total tons 0 0 0 0 0 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0total tons 896.9 259.544 212.345 195 79 139 33 1 1 0 % 0.5 0.1 0.1 0.10.0 0.1 0.0 0.0 0.0 0.0 total tons 0 0 0 0 0 0 0 0 0 0 % 0.0 0.0 0.0 0.00.0 0.0 0.0 0.0 0.0 0.0 total 182648 176431 165486 186474 185970 154906151525 184903 188184 174779 varieties total % 0.6 0.2 0.2 0.7 2.9 3.24.3 4.3 5.8 9.9 Yr7 Cultivated Yr7 varieties 2010 2011 2012 2013 20142015 2016 % virYr7_isolat

24 70 97 92 93 76 92 total tons 10494 9171 8389 6,815.20 6,375.104,858.90 3,076.30 % 5.7 4.7 4.9 4.0 3.9 2.8 1.9 total tons 0 0 0 65.9490.9 197.7 53.9 % 0.0 0.0 0.0 0.0 0.3 0.1 0.0 total tons 10719 99489832 8,161.10 5,903.30 4,664.20 3,326.20 % 5.8 5.0 5.7 4.8 3.6 2.7 2.1total tons 0 0 0 275 11,885.60 17,032.90 17,587.70 % 0.0 0.0 0.0 0.2 7.29.7 11.0 total tons 0 0 0 13.8 9.20 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0total tons 0 0 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 total tons 0 0 00 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 total tons 0 0 0 0 0 0 0 % 0.0 0.00.0 0.0 0.0 0.0 0.0 total tons 0 0 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.00.0 total tons 0 0 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 total tons 00 0 0 0 0 0 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 total tons 0 0 0 0 0 0 0 % 0.00.0 0.0 0.0 0.0 0.0 0.0 total 184795 197221 171034 170,276.70 164,779.00174,991.40 159,371.70 varieties total % 11.5 9.7 10.7 9.0 15.0 15.3 15.1Yr7

indicates data missing or illegible when filed

to draw the plot presented next to the table. The proportion ofharvested Yr7 wheat varieties is shown in dark green and prevalence ofyellow rust isolates virulent to Yr7 in orange (UK, from 1990 to 2016).

TABLE 2 Summary of the newly generated and previously published plantmaterials analysed for the present study with the different PST isolatesused for the pathology assays. Table 2: Plant materials and rustisolated used in the present study Gene Experiment Plant Material Rustisolate Reference(s) Yr7 MutRenSeq EMS-derived TILLING PST 08/21Krasileva et al., 2017 population in the UK Cadenza cultivarConfirmation of the Yr7 Avocet-Yr7 EMS mutants Generated for the studycandidate through sequencing Genetic linkage F₂ population: Generatedfor the study confirmation Cad0127 × CadWT (139) Yr7 KASP primer testingCadenza-derived varities + PST 08/21; PST 15/15

Generated for the study Yr7 carriers Yr7 frequency in UK Recommendedlist 2018 https://cereals.ahdb.org.uk/varieties/ breeding materialsahdb-recommended-lists.aspx Gediflux collecion Reeves et al., 2004Core-set of the Watkins collection Wingen et al., 2014 Yr5 MutRenSeqEMS-derived Lemhi-Yr5 mutants PST81/20 McGrann et al., 2014 Confirmationof the Yr5 Avocet-Yr5 EMS mutants Generated for the study candidatethrough sequencing Genetic linkage F₂ population: Generated for thestudy confirmation Avocet-S × Avocet-S-Yr5 (376) YrSP MutRenSeqAvocet-YrSP EMS mutants 134 E16A+(Culture n

Generated for the study Genetic linkage F₂ population: Generated for thestudy confirmation Avocet-S × Avocet-S-Yr5 (94)

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TABLE 4 Summary of the available genome assemblies that we used for thein silico allele mining and synteny analysis across rice, Brachypodium,barley and different triticeae accessions. Table 4: Genome assembliesthat were used for the present study Specie Cultivar/grou

Source Link/ref Triticum aestivum Cadenza Earlham Institutehttp://opendata.earlham.ac.uk/ Triticum_aestivum/EI/v1.1/ Triticumaestivum Paragon Earlham Institute http://opendata.earlham.ac.uk/Triticum_aestivum/EI/v1.1/ Triticum aestivum Claire Earlham Institutehttp://opendata.earlham.ac.uk/ Triticum_aestivum/EI/v1.1/ Triticumaestivum Robigus Earlham Institute http://opendata.earlham.ac.uk/Triticum_aestivum/EI/v1.1/ Triticum turgidum Kronos Earlham Institutehttp://opendata.earlham.ac.uk/ Triticum_turgidum/EI/v1.1/ Triticumturgidum Svevo The International Durum Wheat http://d-data.interomics.euGenome Sequencing Consortium Triticum turgidum Zavitan WEWseq Avni etal. 2017 Aegilops tauschii Tauschii UC Davis Luo et al. 2017 Oryzasativa Japonica Ensembl/RAP-DB http://plants.ensembl.org/Oryza_sativa/Info/Index Brachypodium distachyon Ensembl/Brachypodium.orghttp://plants.ensembl.org/ Brachypodium_distachyon/Info/Index Hordeumvulgare Morex Ensembl/IBSC http://plants.ensembl.org/Hordeum_vulgare/Info/Index

indicates data missing or illegible when filed

TABLE 5a In silica allele mining for Yr7 and Yr5/YrSP in availablegenome assemblies for wheat Cultivar % ID to Yr5 protein % ID to Yr7protein Cadenza 98.2 100 Paragon 98.2 99.8* Claire 99.3 n.s Robigus 98.2n.s Kronos 93.6 n.s Svevo 93.6 n.s Zavitan n.s n.s *due to the presenceof the Ns in the Paragon sequence (see supp) haplotypes

TABLE 6 List of the identified BED-containing proteins in RefSeq v1.0based on a hmmerscan analysis (see Methods). Several features are added:number of identifed BED domains and the presence of other conserveddomains present, the best BLAST hit from the non-redundant database ofNCBI with its description and score, and whether the BED domain wasrelated to BED domains from NLR proteins based on the neighbour networkshown oi FIG. 10. Table 6: List of the identified BED-containingproteins in RefSeqv1.0 based on a hmmerscan analysis CD- CD- # CD- CD-CD-Search/ Search/ Search/ BED Search/

Search hmmer hmmer

hmme

Best BLAST hit TraesCS1B01G158800.1 1 ZnF_BED DUF4413 Dimer_XP_016740977.1 Tnp_hAT TraesCS3B01G269600.1 1 ZnF_BED DUF4413 Dimer_XP_020177565.1 Tnp_hAT TraesCS3B01G317800.1 1 ZnF_BED DUF4413 Dimer_XP_020177565.1 Tnp_hAT TraesCS5B01G377100.1 1 ZnF_BED DUF4413 Dimer_ABA94812.1 Tnp_hAT TraesCS5B01G501500.1 1 ZnF_BED XP_020164333.1TraesCS5D01G501900.1 1 ZnF_BED XP_020164333.1 TraesCS7A01G447400.1 1ZnF_BED DUF4413 Dimer_ XP_020177565.1 Tnp_hAT BED sequence related toBNLs align- in Neighbour Best BLAST hit description qlength slentgh % IDment Network Tree TraesCS1B01G158800.1 PREDICTED: zinc finger BED 706698 42.837 705 Yes domain-containing TraesCS3B01G269600.1 zinc fingerBED domain- 772 395 94.43 395 yes containing protein RICE

TraesCS3B01G317800.1 zinc finger BED domain- 675 395 92.911 395 yescontaining protein RICE

TraesCS5B01G377100.1 hAT family dimerisation 728 709 58.779 655 yesdomain containing prot

TraesCS5B01G501500.1 protein NLP4-like [Aegilops 663 714 74.965 715 yestauschii subsp. taus

TraesCS5D01G501900.1 protein NLP4-like [Aegilops 715 714 100 714 yestauschii subsp. taus

TraesCS7A01G447400.1 zinc finger BED domain- 772 395 94.937 395 yescontaining protein RICE

indicates data missing or illegible when filed

TABLE 8 List of de novo assemblies generated from the correspondingRenSeq data Table 8: Sequencing data details # Read-pairs EnrichmentSequence mapped to Sample Accession Sequencing chemistry po

pool # Read-pairs # Read-pairs the de novo % Read-pairs do novo assemblyMW01-127_HM7MVBCXX_L1_2.fq.gz Cad0127 Illumina_HiSeq_2500 (

A 1 14805176 14743094 18772686   64% Cadenza-WTMW01-127_HM7MVBCXX_L1_2.fq.gz Cad0127 Illumina_HiSeq_2500 (

A 1 14805176 14743094 MW01-1551_HM7MVBCXX_L1_1.fq.gz Cad1551Illumina_HiSeq_2500 (

A 1 8216218 8184048 10619188   65% Cadenza-WTMW01-1551_HM7MVBCXX_L1_2.fq.gz Cad1551 Illumina_HiSeq_2500 (

A 1 8216218 8184048 MW01-1978_HM7MVBCXX_L1_1.fq.gz Cad1978Illumina_HiSeq_2500 (

B 1 12462294 12409066 15916836   64% Cadenza-WTMW01-1978_HM7MVBCXX_L1_2.fq.gz Cad1978 Illumina_HiSeq_2500 (

B 1 12462294 12409066 WW01-27_Cadenza_S3_L001_R1_001.fastq.gz Cadenza-WTIllumina_MiSeq (250b

C 2 5901019 5843683 7884202   67% Cadenza-WTWW01-27_Cadenza_S3_L001_R2_001.fastq.gz Cadenza-WT Illumina_MiSeq (250b

C 2 5901019 5843683 AvS_KD17010810-A71_HCHT7BCXY_L1_1.fq.gz AvocetSIllumina_HiSeq_2500 (

D 3 12669666 12284950 AvS_KD17010810-A71_HCHT7BCXY_L1_2.fq.gz AvocetSIllumina_HiSeq_2500 (

D 3 12669666 12284950 AvS_SP_KD17010810-A50_HCHT7BCXY_L1_1.fq.gzAvocetS-YrS

Illumina_HiSeq_2500 (

D 3 13559810 AvS_SP_KD17010810-A50_HCHT7BCXY_L1_2.fq.gz AvocetS-YrS

Illumina_HiSeq_2500 (

D 3 13559810 AvS_Yr5_KD17010810-A81_HCHT7BCXY_L1_1.fq.gz AvocetS-Yr5Illumina_HiSeq_2500 (

D 3 10131809 AvS_Yr5_KD17010810-A81_HCHT7BCXY_L1_2.fq.gz AvocetS-Yr5Illumina_HiSeq_2500 (

D 3 10131809 AvS_Yr7_KD17010810-A93_HCHT7BCXY_L1_1.fq.gz AvocetS-Yr7Illumina_HiSeq_2500 (

D 3 7698058 AvS_Yr7_KD17010810-A93_HCHT7BCXY_L1_2.fq.gz AvocetS-Yr7Illumina_HiSeq_2500 (

D 3 7698058 C855_KD17010810-A2_HCHT7BCXY_L1_1.fq.gz Cad0855Illumina_HiSeq_2500 (

E 3 13109055 12568140 17166458   68% Cadenza-WTC855_KD17010810-A2_HCHT7BCXY_L1_2.fq.gz Cad0855 Illumina_HiSeq_2500 (

E 3 13109055 12568140 C903_KD17010810-A94_HCHT7BCXY_L1_1.fq.gz Cad0903Illumina_HiSeq_2500 (

E 3 9109264 8704600 11780688   68% Cadenza-WTC903_KD17010810-A94_HCHT7BCXY_L1_2.fq.gz Cad0903 Illumina_HiSeq_2500 (

E 3 9109264 8704600 C923_KD17010810-A40_HCHT7BCXY_L1_1.fq.gz Cad0923Illumina_HiSeq_2500 (

E 3 14252713 13647531 17530654   64% Cadenza-WTC923_KD17010810-A40_HCHT7BCXY_L1_2.fq.gz Cad0923 Illumina_HiSeq_2500 (

E 3 14252713 13647531 C1034_KD17010810-A49_HCHT7BCXY_L1_1.fq.gz Cad1034Illumina_HiSeq_2500 (

E 3 13415313 12889224 15567764   60% Cadenza-WTC1034_KD17010810-A49_HCHT7BCXY_L1_2.fq.gz Cad1034 Illumina_HiSeq_2500 (

E 3 13415313 12889224 YSP_0_KD17071213-AK3122_HV32GBCXY_L1_l.fq.gzAvocetS-YrS

Illumina_HiSeq_2500 (

F 4 20168141 19285244 25472610 66.04% AvocetS-YrSP-WTYSP_0_KD17071213-AK3122_HV32GBCXY_L1_2.fq.gz AvocetS-YrS

Illumina_HiSeq_2500 (

F 4 20168141 19285244 AvocetS-YrSP-WTYSP_1_KD17071213-AK2489_HV32GBCXY_L1_1.fq.gz AvocetS-YrS

Illumina_HiSeq_2500 (

F 4 4866592 4715938 6208114 65.82% AvocetS-YrSP-WTYSP_1_KD17071213-AK2489_HV32GBCXY_L1_2.fq.gz AvocetS-YrS

Illumina_HiSeq_2500 (

F 4 4866592 4715938 AvocetS-YrSP-WTYSP_2_KD17071213-AK3121_HV32GBCXY_L1_1.fq.gz AvocetS-YrS

Illumina_HiSeq_2500 (

G 4 22067358 21281452 28040118 65.88% AvocetS-YrSP-WTYSP_2_KD17071213-AK3121_HV32GBCXY_L1_2.fq.gz AvocetS-YrS

Illumina_HiSeq_2500 (

G 4 22067358 21281452 AvocetS-YrSP-WTYSP_3_KD17071213-AK2464_HV32GBCXY_L1_1.fq.gz AvocetS-YrS

Illumina_HiSeq_2500 (

G 4 14603831 14068492 18132636 64.44% AvocetS-YrSP-WTYSP_3_KD17071213-AK2464_HV32GBCXY_L1_2.fq.gz AvocetS-YrS

Illumina_HiSeq_2500 (

G 4 14603831 14068492 AvocetS-YrSP-WTYSP_4_KD17071213-AK2483_HV32GBCXY_L1_1.fq.gz AvocetS-YrS

Illumina_HiSeq_2500 (

H 4 16757582 15993630 20438956 63.90% AvocetS-YrSP-WTYSP_4_KD17071213-AK2483_HV32GBCXY_L1_2.fq.gz AvocetS-YrS

Illumina_HiSeq_2500 (

H 4 16757582 15993630 AvocetS-YrSP-WTY5_0_KD17071213-AK2488_HV32GBCXY_L1_1.fq.gz AvocetS-Yr5-Illumina_HiSeq_2500 (

H 4 18106714 17329780 23756414 68.54% AvocetS-Yr5-WTY5_0_KD17071213-AK2488_HV32GBCXY_L1_2.fq.gz AvocetS-Yr5-Illumina_HiSeq_2500 (

H 4 18106714 17329780 AvocetS-Yr5-WTY5_1_KD17071213-AK2485_HV32GBCXY_L1_1.fq.gz AvocetS-Yr5-Illumina_HiSeq_2500 (

I 4 12149902 11617256 14917602 64.20% AvocetS-Yr5-WTY5_1_KD17071213-AK2485_HV32GBCXY_L1_2.fq.gz AvocetS-Yr5-Illumina_HiSeq_2500 (

I 4 12149902 11617256 AvocetS-Yr5-WTY5_2_KD17071213-AK2486_HV32GBCXY_L1_1.fq.gz AvocetS-Yr5-Illumina_HiSeq_2500 (

I 4 18064931 16987606 23153166 68.15% AvocetS-Yr5-WTY5_2_KD17071213-AK2486_HV32GBCXY_L1_2.fq.gz AvocetS-Yr5-Illumina_HiSeq_2500 (

I 4 18064931 16987606 AvocetS-Yr5-WTY5_3_KD17071213-AK2487_HV32GBCXY_L1_1.fq.gz AvocetS-Yr5-Illumina_HiSeq_2500 (

J 4 15563606 14814817 19915922 67.22% AvocetS-Yr5-WTY5_3_KD17071213-AK2487_HV32GBCXY_L1_2.fq.gz AvocetS-Yr5-Illumina_HiSeq_2500 (

J 4 15563606 14814817 AvocetS-Yr5-WT

indicates data missing or illegible when filed

TABLE 9 Sequencing details of RenSeq data generated in this study. Table9: de novo assemblies from RenSeq data statistics de novo assemblyassembler #contigs #NLR-contigs #complete_NLR Cadenza-WT CLC assemblycell 29706 5572 431 AvocetS CLC assembly cell 400158 AvocetS + YrSP CLCassembly cell 530695 AvocetS + Yr7 CLC assembly cell 278126 AvocetS +Yr5 CLC assembly cell 362856 Paragon Kronos AvocetS + YrSP_AU CLCGenomics Wo

268235 5361 791 AvocetS + Yr5_AU CLC Genomics Wo

109608 5180 782

indicates data missing or illegible when filed

TABLE 10 Summary of primers designed for the present study. (Part 1/2)KASP_R- Primer_Name Gene Primer_Type chromosome gene_alleleKASP_alternate_allele common product_size Comment Yr7 detection Yr7-AYr7 KASP 2BL TTAGTCCTGCC TTAGTCCAGCCCATAAGCc CAGTGTT 41 CCATAAGCgAAAACCA GGGAGGA Yr7-B Yr7 KASP 2BL TGGAGGTATCA TGGAGGTATCATCGGGTGAaCATCAAA 70 Dominant TCTGGTGAg ATCATCG marker: CCTATGT alternate allele is actually not amplified Yr7-C Yr7 KASP 2BL CACATGAGTCGCACACGACCTAATACTGAGa ACTGCAA 48 Dominant ATACTGAGg TGCCTTC marker: CCATAalternate allele is actually not amplified Yr7-D Yr7 KASP 2BLGCTGGAAAGGC GCTGGAAAGGCTTGAGATCg AATGGCG 48 TTGACATCa TGGTAAG GACAGAPrimer_Name Forward Reverse Product size Y Product size YrSPAlternate profile Yr5 detection Yr5-Insertion CTCACGCATT TATTGCATAA

1281 507 no amplification Primer_Name Gene Primer_Type chromosomeKASP_WT_allele KASP_mutant_allele common product_size Yr7 mappingCad0127 Yr7 KASP 2BL AAGTGATGTCGGGA AAGTGATGTCGGGAGGAGt TGGAGAATG 83GGAGc GAAGTTCTT TTGTGT Cad1551 Yr7 KASP 2BL CACAATCATCAAGACACAATCATCAAGATGAA CCAACAATA 51 TGAAGCg GCa TCTCAGTTA CCTCATTG Cad1978Yr7 KASP 2BL TGCATCCTTCCAGG TGCATCCTTCCAGGACAA AACCAGGGA 79 ACAAATg ATaGGACGCTTA TG Cad0127_M1 Yr7 mapping KASP 2BL ACATTTACGTGGAGACATATTCGTGGAGGCCGa TGGTGAACT 94 GCCGg CTGATAGGA ACTTC Cad0127_M2Yr7 mapping KASP 2BL TTCTCCTGCGCCTC TTCTCCTGCGCCTCTCTGa GGAGGGTCT 59TCTGg GGCCTCTGT Cad0127_M3 Yr7 mapping KASP 2BL CGGAACCAATCACCCGGAACCAATCACCTCGGa ATGTTGTCC 78 TCGGg ACGGCGATT AA Cat0127_M4Yr7 mapping KASP 2BL GAAAGCAGCAGCCA GAAAGCAGCAGCCACAGt TTGGTCGGC 55 CAGcTCTTGAACT TT Cad0127_M5 Yr7 mapping KASP 2BL CATCATCCATTTTCCATCATCCATTTTCCCTC AGCTTCTTT 51 CCTCTCGc TCGt AGAACATGC CAAC Cac0127_M6Yr7 mapping KASP 2BL ACTGCTCGCAACAC ACTGCTCGCAACACATAC CCCAATTAT 67ATACAc At TTGCAGTGC TTGAG Cad0127_M7 Yr7 mapping KASP 2BL GCTTCAGTGAACAAGCTTCAGTGAACAAGGTG GAGAGGAGA 36 GGTGATGc ATGt AATGACATC CTAGATCad0127_M8 Yr7 mapping KASP 2BL AGAACCAGAGAATT AGAACCAGAGAATTTGTTCGACTATGG 103 TGTTGTTGTAg GTTGTAa AGAACCTTG AGAGA Cad0127_M9 Yr7 mappingKASP 2BL GCCTTTCTTCATCT GCCTTTCTTCATCTGGCC TGTGGTACG 78 GGCCTTTAGcTTTAGt AGTTGGCAT ACC Primer_Name Gene/Name Primer_Type chromosomeKASP_Target KASP_Alt common product_size Yr5 mapping Yr5_candidate Yr5KASP 2BL CAGGAGATCTTG CAGGAGATCT AAACTCTTTGACT 44 AAGGACAT TAAAGGAATAGGTACTCG Yr5_M1 W90K_Kukri_

KASP 2BL ask SEB Yr5_M2 W90K_RAC87 KASP 2BL Yr5_M3 W90K_Tduru

KASP 2BL WMC175 KASP 2BL Yr5_M4 W901_Ra__c6

KASP 2BL Yr5_M5 W90K_GENE-

KASP 2BL Yr5_M6 W90Kt_wsnp_

KASP 2BL YrSP mapping Yr5_candidate YrSP KASP 2BL CAGGAGATCTTGCAGGAGATCTT AAACTCTTTGACT 44 AAGGACAT AAAGGAATA GGTACTCG YrSP_M1W90K_JD_c2

KASP 2BL YrSP_M2 RAC875_rep_

KASP 2BL Yr5P_M3 BobWhite_c3

KASP 2BL

indicates data missing or illegible when filed

TABLE 10 Summary of primers designed for the present study. (Part 2/2)Primer name Forward Reverse product size (bp) Yr7 cloning Yr7_locusAGCCAGCAGAAGTCTTAGAAACAG CTACGAGATATATGTTGAGCAGCTTG 6.6 kb ATCTTAGAAACAGCCACGTC ACGTCGATCAAACAGAGG 704 B TTGTACTTCGGCATCCTCACACTTCGCTTTCACTGG 709 C TCAATCTTTGGGTTGTGC TGTGCCGAAAAGAAACAT 791 DCTGAGGTCGAGAGAGTCG TTTCCGTTGGACGAACTA 746 E CTGATAACCAACCCACCACGCGAAGTTGTTAATTCC 702 F GATCCAGCGCTACTTCAA AACGGATTGCCCTTTAAC 829 GTTGTCTGTTGCACAAAGGT AGGAATGTTCCCCTTCAG 728 H AAGAATTGGATGGGGAAGATAAGCGTCCTCCCTGGT 784 I CTACCCAATGGCTTGTTG GCCATGATCCCTGAATG 768 JAGGTGAAGTTGAGCAGCA CATCAGCGATAGCCACTT 713 K CAGATGTGACGGCAGAGTGTTGCGTGCCCTCTAGTA 734 L AGAAACGCTGCAAGTCTG CTGAAACGCTCATTCTGG 792Yr5 cloning Yr5_locus CGCTTAATTCCCCTTCCTTC CACGTCAGACTGGATCAAAGCTCTA4.9 kb A Yr5_locus_F TGGCTCCTTATTCGTTCTCTTTC 813 B GGGAACACTTCACGATCAAATTCCTTCATGCCTTCC 901 C CTTGCTCCAAGGAAAGTG CCCTGTGACATCCAGAAA 890 DAGGGAAACCCACTAGCAG TGGTTGCAATGGAAGAGT 900 E GTGTGCTGCAAATGTCTGATGACCTCTGCCCAGTTT 819 F GAGAAACCTGCCCAAAGT ATGGTATGCGCAACAGTC 884 GGGTTGCCGGAATCTAAGT GATGGGTCTTGGATGTGA 890 H GCAACCCTGCTTTCCTAGCYr5_locus_R 671

TABLE 18 Corresponding gene models NLR Annotator Longest overlap inEnsembl BLASTx best hit comments Os1 LOC_Os04g52970.1.1 Os2Os04t0621500-00_LOC_Os04g53030.1.1 Os3 Transcript: LOC_Os04g53040.1.1Os4 Transcript: LOC_Os04g53050.1.1 && Transcript: LOC_Os04g53060.1.1 Os5Transcript: LOC_Os04g53120.1.1 Os6 Transcript: LOC_Os04g53160.1.1 Bd1BRADI_5g22145v3 Phytozome: Bradi5g22146.1 Bd2 BRADI_5G22160.1 &&truncated genes so kept Annotator BRADI_5G22160.1 locus Bd3BRADI_5g22179v3 Bd4 BRADI5G22187 Hv1 HORVU2Hr1G103460.1 XP_020186889.1Traces of BED but not annotated as such by CD search Hv2HORVU2Hr1G103440.1 truncated gene so kept Annotator locus Aet1 EMT18301Aet2 X EMS51583.1 kept Annotator locus Aet3 EMT06562 Aet4 EMT29760 Aet5EMT12526 Aet6 EMT02111 Aet7 EMT18676 Aet8 EMT12939 Tt1 TRIDC2BG071010.1EMS62808.1 Tt2 TRIDC2BG071030.1 EMS62808.1 no conserved domain in genemodel Tt3 X kept Annotator locus Tt4 TRIDC2BG071040.1 Tt5 X EMS51583.1kept Annotator locus Tt6 TRIDC2BG071050.1 EMS51583.1 Tt7 X keptAnnotator locus Tt8 TRIDC2BG071070.1 CAD45026.1 Tt9 TRIDC2BG071070.18EMS62808.1 kept Annotator locus Tt10 TRIDC2BG071180.3 XP_020186889 Tt11X kept Annotator locus Tt12 TRIDC2BG071220.1 XP_020186937.1 no conserveddomain in gene model Tt13 X XP_003579311 Tt14 TRIDC2BG071240.1XP_020186937.1 Tt15 X XP_003579311.1 kept Annotator locus Tt16 XXP_014751374.1 kept Annotator locus Tt17 X XP_003579311.1 kept Annotatorlocus Tt18 X BAJ98893.1 kept Annotator locus Tt19 X KQJ84588.2 keptAnnotator locus Tt20 TRIDC2BG071280.1 XP_003579311.1 Ta_2A1TraesCS2A01G464500 Ta_2A2 TraesCS2A01G464700 Ta_2A3 TraesCS2A01G464900Ta_2A4 X partial NLR kept Annotator locus Ta_2A5 TraesCS2A01G465100Ta_2A6 TraesCS2A01G465200 Ta_2A7 TraesCS2A01G465600 Ta_2A8TraesCS2A01G466100 Ta_2A9 X XP_020186937.1 kept Annotator locus Ta_2A10TraesCS2A01G625200LC partial gene model kept Annotator locus Ta_2A11TraesCS2A01G625400LC- kept Annotator locus TraesCS2A01G625500LC-TraesCS2A01G625600LC Ta_2A12 TraesCS2A01G466500- kept Annotator locusTraesCS2A01G625600LC- TraesCS2A01G466600 Ta_2D1 TraesCS2D01G465300Ta_2D2 TraesCS2D01G465400 Ta_2D3 TraesCS2D01G465500 Ta_2D4TraesCS2D01G465600 Ta_2D5 TraesCS2D01G466000 Ta_2D6 TraesCS2D01G466400Ta_2D7 TraesCS2D01G466600 Modified gene model rescued one additional BEDdomain Ta_2B1 TraesCS2B01G486100 Ta_2B2 TraesCS2B01G485200 Ta_2B3 Xpartial NLR kept Annotator locus Ta_2B4 TraesCS2B01G486300 Ta_2B5 Xpartial NLR kept Annotator locus Ta_2B6 TraesCS2B01G486400 Ta_2B7TraesCS2B01G486700 Ta_2B8 TraesCS2B01G487700 Ta_2B9 TraesCS2B01G488000Ta_2B10 TraesCS2B01G488400 Ta_2B11 TraesCS2B01G488600-TraesCS2B01G488700 Ta_2B12 TraesCS2B01G734100LC Ta_2B13TraesCS2B01G489400

SELECTED SEQUENCE INFORMATION >Yr7_locus  (SEQ ID NO: 5)

TCGGTTCTCGGTTCTCGGTTTTCGGGTTTGTGAAGCCTCTGACCCTGGCATTTGCTCGGGTTCGGTTCTGCTCTAGGTGCCTACTGGCTACGGCCAACGCGCCTCCTGTCGGGGCGGTTTTCCACGCAACTTAGCATCCGGCAACTTATATATAACAAACCTGCGTTCCTTCTTCTCGCTCCACCGGTTTCCAAGCTCAGAGCTTCAAGCCAAACCCATTTCCAGTGAAGCAGTCGATGGAGCTCCTCACCTTCCTCTTCAGAATGGTGGCCCTGATCCCCGGCGCATTACGCAACGCGGAGAAGCTGCCCGGTGCTCTCATCTCGTGCGGCGTCGTCCAAGCCGCGGCGGCGCTCTTCC

TGGTATTCGGGCTTGTGGAGGCGTCCGCCGGATTTTATGTGTCCGGCGATGTGGCCGGACGCCGTGCTGCCGGGAAGACCATCCTGTGGG

CCCGTTCATGTTGTATAGAATATAATGAGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGGGGGGCCATTTTGGTCAGTGTGTGCTTTGGGGACGGGGGAATCAGTAGTAGGTTGTACCAGCACGAGTGTTTTAGACTTCATATACTTTCATTCTTTTTTTCACTTGA

CTTCTCATGCCGTGTTCGGGCCGTATTCTCGAGCATAAAGTTCGGCCCACTAAGTGTCGAAAGAAAGCTGCTTCTAATTGACCTTCTGCT

TGTGTTGTGGCTGGTGTTCTTCCCCGCTCGTCTCGTCTGCTCCCCATTCCACACGCTTAATTCCCCTTCCTTCATTGACTCGAGCTCGAGACCTGCTCCTGCCGGATCTGATAATGGAGCCGGCGGGAGACTCTTCCCTGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAA

ACACGGTGGTGGCTGCTGTGAAGGGGAGGGCAGCCGGGAACATGCCTCTGTCCCGGTCTCTCGCTCGTGTCAAGGAGCTTCTCTATGACGCCGACGACGTGATCGACGAGCTAGACTACTACAGGCTCCAACACCAAGTCGAAGGAGGTAGTAAGCATAATCCCATTATATATCGAATCT

GCTGAAAGAGTGGATGAAATATCAAGGGGCCATGTCGATACACTGAATGTCAGTGTTGGCAAATTACGGTCCCCGGTATGGGAACACTTCACCATCACAGAAACAACTATCGACGGGAAGCGTTCAAAAGCCAAATGTAAGTACTGTGGAAATGATTTTAATTGCGAAACGAAGACAAACGGGACTTCATCTATGAAAAAACATTTGGAGAAGGAGCATTCCGTGACTTGCACGAATAAATCTGCAGTGCACCCCCCAAACACTTCAAGGTACCAGCAGGAATTTATACCTTGCTTCAACGAATTTGTTGTAATTGTTTATATACGTCTGCTTGAGAGCCCATTGTTGTTCTGAATTTCT

CAGAAAAGAATAAGATCAAAAAGTCAATAACTGAAAACCAATCTGGTGGTGTAAATGTTCTGCCTATTGTAGGCATTGCAGGTGTTGGAAAGACAACTCTTGCTCAATTTGTGTATAATGATCCAGACGTGAAAAGTCAATTTCACCACAGGATATGGGTTTGTGTGTCCTGCAAATTTG

TGAAAGAACATGTCGAGTACCAAGCAAAGAGTTTTCTGCTCATTTTAGATGATGTCTCGGACAGTATGGATTATCATAAATGGAACAAAT

AACCGATCAAGTTAGGTGCTTTAGAAAACGATGATATGTGGTTATTGCTCAAGTCATGTGCATTTGGTTTTGGGAACTATGAAGGTACGG

ATCTTAGCATTGATCATTGGAGTAACATTCTCAAGAATGAGAAGTGGAAATCGCTGGGACTCAGTGGGGGCATCATGCCTGCTTTGAAGCTTAGTTATGATGAGTTGACGTACCGTTTACAACAATGTTTCTCGTATTGCTCTATATTTCCTGACAAATATAGGTTTCTCGGGAAGGATTTGGTCTATATTTGGATTTCTCAGGGATTTGTGAATTGCACCCAAAATAAGAGATTGGAGGAGACGGGATGGGAATATCTGAATCAATTGG

TGTGTGATCTCATGCATGATTTCGCAAGGATGATTTCAAGGACTGAATGTGCGACTATAGATGGTCTACAGTGCAATAAAATATTCCCAA

TGAGAAATTCAGTTACATCAGTTACCAAATTGAGAACATTGGTTGTGCTTGGGAACTTTGACTCTTTCTTTGTACGGTTGTTCCAAGATATATTCCAGAAGGCACAAAATTTACGCCTGCTGCTAGTATCTCTAGCATCCACTTATCTGTCTCAAGTGCCTGCTGCATTCAATGATTTTAATTCCTTCCTGTGCAATTTGGCAAATCCTTTGCATCTTCGTTACCTAAAACTTGAGTTGGATGGGATTGTGCCACAAGTTTTGAGTACGT

TTGTTGCACACAAGAGAGTCCATTCTTCCATTACTAGCATTGGTAACATGACATCTATCCAGGAGCTACATGATTTTGAAGTTCGAATTT

GTGACACTGAATTTGAATCTTCTGCAAACATGGCAAGAGAAGTGATTGAGGGTCTTGAACCACACATGGATTTAAAACATCTACAAATATCTCAGTATAATGGTACCACTTCACCAGCTTGGCTTGCCAACAATATCTCAGTTACCTCATTGCAGACGCTTCATCTTGATGATTGTGGAG

CTTCACTGGAGGAGCTAGTTCTAATTAAAATGCCGAAGTTAGTGAGATGCTCAAGCACTTCTGCCGAGGGTCTGAGCTCTAGCTTAAGGG

CTGGTCTTAGGAATTTGATTCTATATTGTTGCCCTCATTTGAAAGTGTTGAAGCCTCTTCCACCTTCAACTACCTTTTCTAAGGTACTCATCAGAGAAATTTCAAGATTTCCGTCTATGGAGGTATCATCTGGTGAGAAGTTACAAATTGGGAATATTGATGTGTACATAGGCGATGATTTTGATGAGTCTTCTGATGAGTTGAGCATACTGGATGACAAAACTTTGGCGTTCCATAATCTTAGAAACCTGAAATCGATGGAGATATATGGTTGCAGAAATCTAAGGTCTTTTTCGTTCGAAGGTTTCAGTCATCTTGTCTCTTTAACAAGTTTGAAAATAGTAAGCTGTGAACAACTTT

TAACAAGAGTAGTGTTACCGATGGAAGAGGAAGAAAACAATCTATTAACAACAGTACTGTCATCAGGAAATCAAGATGAGGCATTGACATGGTTAGTTCGTGACGGACTCTTGCACATTCCATCAAATCTCGTCTCCTCTCTCAAGAATATGAGTATTACTCAGTGCCCTCGCCTAAAGTTTAACTCAGGCAAGGACTGCTTCTCTGGATTTACCTCGCTTGAGAAGCTTGAAATTTGGGGATCGTTGGTGGATGATGACGGAAGTGATGACCTGGAGAATGGAAGTTCTTTTGTGTTCGGAGAGGAGGATCAACCCCTGGGGGCGAACGGAAGATGGCTCCTCCCGACATCACTTCAGG

CCGGCCAAGGTTTGCAATCTCTACAGCTGTACTCATGCACGGCACTGGAAGAATTGGCAATTTCCGGCTCTGGATCGGTCACCGTCACTG

GGTTGTGCCCTCGGCTGGAAAGGCTTGACATCAATGACCCATCTGTCCTTACCACGCCATTCTGCAAGCACCTCACCTCCCTGCAACGCCTAAAACTTGGCTTCTTGAAAGTGACGAGACTAACAGATGAGCAAGAACGAGCGCTTGTGCTCCTCAAGTCACTGAAAGAGCTCGAGATTTTTTATTGTACTCATCTCATAGATCTTCCTGCGGGGCTGCAGACCCTTCCTTCCCTCAAGAGTTTGAAGATAGAAGAGGGTCGAGGCATCTCAAGGCTGCCGGAAGCAGGCCTCCCACATTCGCTGGAAGAACTGGAAATCAAAATTTGCAGCAAGCTAGAAGATGAATGCAGGCGGCTAGCAACATGCGAAGGCAAGCTAAAAGTCAAAATTGATGGTCGATATGTGAATTAATTATGTTTCTGGCCTCATGTGCAAAGTGTACCGCTTG

>Yr7_CDSATGGAGCCGGCGGGAGACTCTTCCCTGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAACGCTCCTCATGGACAAGATGGAGGCCTGGATTCAGCAAGTCGGGCTTGCCGACGACGTCGAGAGGCTCCAGTCTGAGGTCGAGAGAGTCGACACGGTGGTGGCTGCTGTGAAGGGGAGGGCAGCCGGGAACATGCCTCTGTCCCGGTCTCTCGCTCGTGTCAAGGAGCTTCTCTATGACGCCGACGACGTGATCGACGAGCTAGACTACTACAGGCTCCAACACCAAGTCGAAGGAGTTACAAGTGACGAGCCTGACGGTATGCGTGGAGCTGAAAGAGTGGATGAAATATCAAGGGGCCATGTCGATACACTGAATGTCAGTGTTGGCAAATTACGGTCCCCGGTATGGGAACACTTCACCATCACAGAAACAACTATCGACGGGAAGCGTTCAAAAGCCAAATGTAAGTACTGTGGAAATGATTTTAATTGCGAAACGAAGACAAACGGGACTTCATCTATGAAAAAACATTTGGAGAAGGAGCATTCCGTGACTTGCACGAATAAATCTGCAGTGCACCCCCCAAACACTTCAAGCACCGGCGATGCTACTTGTAATGTGAGGTCGGTTGAAGTTGGTAGTTCGTCCAACGGAAAAAGAAAGAGAACAAATGAGGATCCAACGCAGACCACCGCAGCTAACATACACGCCCAATGGGACAAGGCTGAGTTATCCAATAGGATAATTAAAATTACTGAGAAGTTACAGTTACAGGACATCCAGGGGGCTTTGAGTAAAGTTCTCGAGCCATATGGATCCAGCGCTACTTCAAGTTCAAATCATCACCGCTTGAGTACAGCATCGAATCAGCACCCAACAACATCAAGTCTTGTTCCAATGGAAGTTTATGGCAGAGTTGCAGAAAAGAATAAGATCAAAAAGTCAATAACTGAAAACCAATCTGGTGGTGTAAATGTTCTGCCTATTGTAGGCATTGCAGGTGTTGGAAAGACAACTCTTGCTCAATTTGTGTATAATGATCCAGACGTGAAAAGTCAATTTCACCACAGGATATGGGTTTGTGTGTCCTGCAAATTTGATGAAGTGAAGCTCACAAAGGAGATGTTAGACTTTTTTCCTCGAGAAAGGCATGAAGGAATTAACAACTTCGCGAAGCTTCAAGAGATCTTGAAAGAACATGTCGAGTACCAAGCAAAGAGTTTTCTGCTCATTTTAGATGATGTCTCGGACAGTATGGATTATCATAAATGGAACAAATTGTTGAACCCTTTGCTATCAAGTCAAGCGAAGAATATAATTCTAGTCACGACCAGAAATTTGTCTGTTGCACAAAGGTTAAGCACACTTGAACCGATCAAGTTAGGTGCTTTAGAAAACGATGATATGTGGTTATTGCTCAAGTCATGTGCATTTGGTTTTGGGAACTATGAAGGTACGGAAAATCTAAGCACTATTGGAAGACAAATAGCAGAGAAGTTAAAGGGCAATCCGTTAGCAGCAGTAACTGCAGGGGCACTGTTAAGAGATAATCTTAGCATTGATCATTGGAGTAACATTCTCAAGAATGAGAAGTGGAAATCGCTGGGACTCAGTGGGGGCATCATGCCTGCTTTGAAGCTTAGTTATGATGAGTTGACGTACCGTTTACAACAATGTTTCTCGTATTGCTCTATATTTCCTGACAAATATAGGTTTCTCGGGAAGGATTTGGTCTATATTTGGATTTCTCAGGGATTTGTGAATTGCACCCAAAATAAGAGATTGGAGGAGACGGGATGGGAATATCTGAATCAATTGGTAAATCTTGGATTCTTTCAACAAATTGAAGAACAACAAGAATTGGATGGGGAAGAAGAATTCTCTCTACGCCGTCAGATTTGGTACTCTATGTGTGATCTCATGCATGATTTCGCAAGGATGATTTCAAGGACTGAATGTGCGACTATAGATGGTCTACAGTGCAATAAAATATTCCCAACTGTACAGCATTTGTCAATAGTAACCGGTTCTGCATACAACAAAGATCTGAAGGGGAACATTCCTCGTAATGAGAAGTTTGAAGAAAATATGAGAAATTCAGTTACATCAGTTACCAAATTGAGAACATTGGTTGTGCTTGGGAACTTTGACTCTTTCTTTGTACGGTTGTTCCAAGATATATTCCAGAAGGCACAAAATTTACGCCTGCTGCTAGTATCTCTAGCATCCACTTATCTGTCTCAAGTGCCTGCTGCATTCAATGATTTTAATTCCTTCCTGTGCAATTTGGCAAATCCTTTGCATCTTCGTTACCTAAAACTTGAGTTGGATGGGATTGTGCCACAAGTTTTGAGTACGTTTTTTCATCTTCAAGTATTAGATGTTGGATCAAGCATGGATACTTCTCTACCCAATGGCTTGTTGCATAATCTTGTTAGCCTGCGACATCTTGTTGCACACAAGAGAGTCCATTCTTCCATTACTAGCATTGGTAACATGACATCTATCCAGGAGCTACATGATTTTGAAGTTCGAATTTCTAGCGGCTTTGAGATAACACGACTCCAATCCATGAACGAGCTTGTTCAACTTGGGTTGTCTCAACTTGACAGTGTTAAAACCAGGGAGGACGCTTATGGGGCAGGACTAAGAAACAAGGAACACTTAGAAGAGCTTCATTTGTCCTGGAAGGATGCATATTCAGAGTATGAGTATGCCAGTGACACTGAATTTGAATCTTCTGCAAACATGGCAAGAGAAGTGATTGAGGGTCTTGAACCACACATGGATTTAAAACATCTACAAATATCTCAGTATAATGGTACCACTTCACCAGCTTGGCTTGCCAACAATATCTCAGTTACCTCATTGCAGACGCTTCATCTTGATGATTGTGGAGGATGGAGAATACTTCCATCTCTGGGAAGTCTTCCATTCCTTACAAAGGTGAAGTTGAGCAGCATGCTGGAAGTAATTGAAGTACTGATTCCTTCACTGGAGGAGCTAGTTCTAATTAAAATGCCGAAGTTAGTGAGATGCTCAAGCACTTCTGCCGAGGGTCTGAGCTCTAGCTTAAGGGTACTGCACATTGAGGATTGTGAAGCATTGAAGGAGTTTGATCTGTTTGAGAACGATTATAATTCTGAAATCATTCAGGGATCATGGCTGCCTGGTCTTAGGAATTTGATTCTATATTGTTGCCCTCATTTGAAAGTGTTGAAGCCTCTTCCACCTTCAACTACCTTTTCTAAGGTACTCATCAGAGAAATTTCAAGATTTCCGTCTATGGAGGTATCATCTGGTGAGAAGTTACAAATTGGGAATATTGATGTGTACATAGGCGATGATTTTGATGAGTCTTCTGATGAGTTGAGCATACTGGATGACAAAACTTTGGCGTTCCATAATCTTAGAAACCTGAAATCGATGGAGATATATGGTTGCAGAAATCTAAGGTCTTTTTCGTTCGAAGGTTTCAGTCATCTTGTCTCTTTAACAAGTTTGAAAATAGTAAGCTGTGAACAACTTTTCCCTTCAGATGTGACGGCAGAGTATACCCTTGAAGATGTGACAGCTGTGAACTGCAATGCCTTCCCATATCTTAAAAGCCTCAGTATCGACTCATGTGGAATAGCGGGGAAGTGGCTATCGCTGATGCTGCAGCATGCGCCAGGCCTAGAGGAATTGAGTTTAACAAGTTGCGCCCATATAACAAGAGTAGTGTTACCGATGGAAGAGGAAGAAAACAATCTATTAACAACAGTACTGTCATCAGGAAATCAAGATGAGGCATTGACATGGTTAGTTCGTGACGGACTCTTGCACATTCCATCAAATCTCGTCTCCTCTCTCAAGAATATGAGTATTACTCAGTGCCCTCGCCTAAAGTTTAACTCAGGCAAGGACTGCTTCTCTGGATTTACCTCGCTTGAGAAGCTTGAAATTTGGGGATCGTTGGTGGATGATGACGGAAGTGATGACCTGGAGAATGGAAGTTCTTTTGTGTTCGGAGAGGAGGATCAACCCCTGGGGGCGAACGGAAGATGGCTCCTCCCGACATCACTTCAGGAACTTCACATCGTGTCATTGTATTGCCAAGAAACGCTGCAAGTCTGCTTCCCTAGAGATATCACCAGCCTTAAAAAGTTAAGTGTACGTTCCGGCCAAGGTTTGCAATCTCTACAGCTGTACTCATGCACGGCACTGGAAGAATTGGCAATTTCCGGCTCTGGATCGGTCACCGTCACTGTACTAGAGGGCACGCAACCCGCTGGCAGCCTCGGGCGTTTGAATGTATCAGACTGTCCTGGCTTGCCATCACGTTTGGACAGCTTTCCAAGGTTGTGCCCTCGGCTGGAAAGGCTTGACATCAATGACCCATCTGTCCTTACCACGCCATTCTGCAAGCACCTCACCTCCCTGCAACGCCTAAAACTTGGCTTCTTGAAAGTGACGAGACTAACAGATGAGCAAGAACGAGCGCTTGTGCTCCTCAAGTCACTGAAAGAGCTCGAGATTTTTTATTGTACTCATCTCATAGATCTTCCTGCGGGGCTGCAGACCCTTCCTTCCCTCAAGAGTTTGAAGATAGAAGAGGGTCGAGGCATCTCAAGGCTGCCGGAAGCAGGCCTCCCACATTCGCTGGAAGAACTGGAAATCAAAATTTGCAGCAAGCTAGAAGATGAATGCAGGCGGCTAGCAACATGCGAAGGCAAGCTAAAAGTCAAAATTGATGGTCGATATGTGAATTAA >Yr7_protein (SEQ ID NO: 3)MEPAGDSSLEAAIAWLVQTILATLLMDKMEAWIQQVGLADDVERLQSEVERVDTVVAAVKGRAAGNMPLSRSLARVKELLYDADDVIDELDYYRLQHQVEGVTSDEPDGMRGAERVDEISRGHVDTLNVSVGKLRSPVWEHFTITETTIDGKRSKAKCKYCGNDFNCETKTNGTSSMKKHLEKEHSVICTNKSAVHETNTSSTGDATCNVRSVEVGSSSNGKRKRTNEDDTQTTAANIHAQWDKADLSNRIIKITEKLQLQDIQGALSKVLEPYGSSATSSSNHHRLSTASDQHPTTSSLVPMEVYGRVAEKNKIKKSITENQSGGVNVLPIVGIAGVGKTTLAQFVYNDPDVKSQFHHRIWVCVSCKFDEVELTKEMLDFFPRERHEGINNFAKLQEILKEEVEYQAKSFLLILDDVSDSMDYEKWNKLLNPLLSSQAKNIILVTTRNLSVAQRLSTLEPIKLGALENDDMWLLLKSCAFGFGNYEGTENLSTIGRQIAEKLKGNPLAAVTAGALLEDNLSIDEWSNILKNEKWKSLGLSGGIMPALKLSYDELTYRLQQCFSYCSIFPDKYRFLGKDLVYIWISQGFVNCTQNKRLEETGWEYLNQLVNLGPPQQIEEQQELDGEEEPSLRRQIWYSMCDLMHDFARMISRTECATIDGLQCNKIFETVQHLSIVTGSAYNKPLKGNIPRNEKKEDNMRNSVISVTKLRTLVVLGNED

LHNLVSLRHLVAHKRVHSSITSIGNMTSIQELHDPEVRISSGFEITRLQSMNELVQLGLSQLDSVKTREDAYGAGLRNKEHLEELHLSWKDAYSEYEYASDTEFESSANMAREVIEGLEPHMDLKHLQISQYNGTTSPAWLANNISVTSLQTLHLDDCGGWRILPSLGSLPFLTKVKLSS

SGNQDEALTWLVADGLLHIPSNLVSSLKNMSITQCPRLKFNSGKDCFSGFTSLEKLEIWGSLVDDDGSDDLENGSSFVFGEEDQPLGANGRWLLPTSLQELHIVSLYCQETLQVCFPRDITSLKKLSVRSGQGLQSLQLYSCTALEELAISGSGSVIVTVLEGTQPAGSLGRLNVSDCPGLPSRLDSFPRLCPRLERLDINDPSVLTTPFCKHLTSLQRLKLGFLKVTRLTDEQERALVLLKSLKELEIPYCTHLIDLPAGLQTLPSLKSLKIEEGRGISRLPEAGLPHSLEELEIKICSKLEDECRRLATCEGKLKVKIDGRYVN- >Yr5_locus (SEQ ID NO: 4)ATGGAGCCGGCGGGAGACTCTTCCGTGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAACGCTCCTCATGGACAAGATGGAGGAGTGGATTCGGCAAGTCGGGCTTGCCGACGACGTCGAGAGGCTCCAGTCTGAGGTCGAGAGAGTCGACACGGTGGTGGCTGCTGTGAAGGGGAGGGCAGCCGGGAACAGGCCTCTGTCCCGGGCTCTCGCTCGTGTCAAGGAGCTTCTCTACGACGCCGACGACTTGATCGACGAGCTAGACTACTACAGGCTCCAACAACAAGTCGAAGGAGGTAGTAAGCATAATCCCATTATATATCGAATATATGTAAGCTCAAGATATTTATTTTGGGATGGAGGGAGTAGTTTGATCTTAATTTCTGGTCCATATTTTTTTCGGCACAGTTACGAGTGACGACCCTGACGGTATGCGTGGAGCTGAAAGAGTGGATGAAATATCAAGGGGCCATGTCGATACACTGAATTGCAGTGTTGGCAAATTACGATCCCCGGTATGGGAACACTTCACGATCACAGAAACAACTATCGACGGGAAGCGTTCAAAAGCCAAATGTAACTACTGTGGAAATGATTTTAATTGCGAAACGAAGACAAACGGGACTTCATCTATGAAAAAACATTTGGAGAAAGAGCATTCCGTGACTTGTACGAAGAAACCTGGAGCCCATCCACCAAACCCTTCAAGGTACCCAAAGGAAATTATATGTTGCATCAGCGCATTTATATTCGTTTATATATATCTGCTTGAGAGCCCATTGTTGTTCTACATTTCTTCTGATAACTGACCCACCATTTTCTCTCTTAATGCAGCACCGGCTATGCAACTGAAAATGTGACGCTTGTTGAAGTTGGTAGTTCATCCAACAGAAAAAGAAAGAGAACGAATAAGGAGCCAGCACAAACCACCGCAGATAACACCCGTTGGGACAAGGCTGAGTTATCCGATACAATAAAAAAGATTACTAGCCAGTTACAGTTACAGTTACAGGGTATCCTATGGGCTTTCAGTAAAGTTCTCGAGCCACATGGGTCTAGCTCTGCGTCGAGTTCAAATCATCACCAACCGAGTACAACCTCAGATCAGCACGCAAAAACATCAAGTCTTGCTCCAAGGAAAGTGTATGGCAGAGTAGCAGAAATGAACTCCATCAGAAATTTAATAGCAGAAAAGAAATGTGATGCTCTAACTGTTCTGCCTATTGTGgGCATTGCTGGTGTTGGAAAGACAACTCTCGCTCAATCTGTATACAATGATCCAGATATAAAAAGTCAATTTCACCACAAGATATGGGTTTGCGTGTCCCGCAAATTTGATGAAGTGATGCTCACAAGGGAGATGTTAGACTTTGAAAGACACGAGGGATCTCCTCATGAAAATGGAAGGCATGAAGGAATTAGTAGCCTTGCTAAGCTTCAGGAGATCTTGAAGGACATTATCGAGTACCAGTCAAAGAGTTTTCTGCTTATTTTAGATGATGTATGGGACAGTATGGATGATCATCAATGGAGAAAACTGGTGTGTCCTTTTGTATCAAGTCAAGCAAAGGGTAATTTAATTCTAGTCACAACCAGAAATTTGTCAGTTGCACACATGTTAGGAACACGTGAGCCGATAAAGTTGGGTGCTTTGGAAAATGATGTTATGTGGTTGCTGCTCAAGTCATGTGCATTTCGTGATGTGAATTATGAAGGGAACCAAAGTCTAAGCATTGTCGgGAGGCAAATATCAGAGAAGTTAAAGGGAAACCCACTAGCAGCAGAAACAGCGGGGGCACTATTAAGGAAGAAATTTAGCATTGATTATTGGAAAATCATTTTAAAGAATGAAGACTGGAAATCCATGGAGCTCGGTAATGGAATCATGGCTGCTCTAAAGCTTAGCTATGATCAACTTCCCTACCATTTACAACAATGTTTCTCATATTGCTCCATATTCCCCGACGGTTATCAGTTTCTTGGTGAGGAGTTGGTCGGTTTCTGGATGTCACAGGGATTTGTAAAGTGCAACAACTCTAGTCAGAGATTGGAGCAGATAGGACAGTGCTATCTGATTGATTTGGTTAACTTAGGCTTCTTTGAAGAAGTTAAAAGAGAAGAACCATATCTGGGCTGTCGAGTTATGTATGGCATATGTGGTCTCATGCATGATTTTGTGATTATGGTGTCAAGGACTGACTGTGCAAGTATAGATGGTCTGCAGCGCAACAAAATGCCTCAAACTCTACGACATTTGTCAATAGTAACTGGATCCGCGTACAAGAAAAATCAGCACGGAAACATTCCTCGTAATAATAGGTTTGAAGAAAATCTGAGAAATACAATTACATCAGTTAGCGAGTTGAGGACATTGGTGTTACTTGGGCATTATGACTTTTCCTTCTTACTATTATTCCAAGATATATTTCAAAAGGCACATAACTTACGTGTGCTGCAAATGTCTGCAGCACCTGCTGATTTTCTCAAACATAGGTTTGAGGAGGTGGATGGGTCTTTCCCTCAAATTTTGAGCAAATTGTACCATCTCCAAGTATTAGACGTCGGTGCATACACTGATCGTACTATGCCTGGTTGTATTGATAATCTTGTTAGCCTGCGGCATCTTGTTGTACACAAGGGAGTGTACTCTTCCATTGCAACCATTGATAATATGCTATCATTTCAGGAACAACATGGTTTCAAGTTTCATATTTCTAGTGGCTTTGAGATAACACGACTCCAATCCACTGAACATTGGATGCATGTTGATACTCTGGAAGATGTTTATGAGGCAGGACTGGTAAACAATGAACTCTCAGAAAAGTTGCACCTGTCCTGGAAGGATTCTCCTGAGGACATAGGCATGGAGGTTGAGGATTGGGAACCACATTGGGACTTAAGGGTTcTCGAGATATCTGGGTATAATTTTGGTTCGCCAATTGTGGTTGACATCATTATCTTGGTTACATCCTCCCAGACGGTTGAGATATCCAATTGTAGTGAATGGAAAATACTTCCATCTTTGGAAAGATTTCAGTTTTTGACAAATCTGGAGTTGAGAAACCTGCCCAAAGTAATAGAAATACTGGTTCCTTCACTGGAGGAGCTAGCATTAGTTACAATGCCAAAGTTGAAGAAATGTTCATGCACTCCCGTGGAAGGTATGAGCTCTAGACTAAGAGCACTGCGGATCGAGGATTGTCAATCACTGAAGGAGTTTGATCTGTTTGAGAACAATGATAAATTCGAAACTGGGCAGAGGTCATGGGCTCCTAGTCTTAGGGAACTAAGTCTGGAGAATTGCCCCCATTTGAAAGTGTTGAAGCCTCTTCCACTCTCACTCATGTGTTCTGAGTTACTCATAAGTGGAGTTTCAACACTTCCGTACATGAAGGGGTCATCTGATAGAAAGTTATGTATTGGGTATGATGATAAGTATGACTACTATGGTTTTGACGAATCTTCCgATGAGTTGAAGATACTGGATGACAAAATTTTTATGTTCCATAATCTGAAAAACCTCAAATCAATGGTGATATATGGTTGCCGGAATCTAAGTTCCATTTCGTTAAAAGGTTTTAGTTACCTCATCTCTTTAACGAGCTTGGAAATAAGAGACTGTGAAAAACTTTTTGCTTCAGATGAGATGCCAGAGCATACCCTTGAAGATGTGACACCTGCGAATTGCAAGGCTTTCCCATCTCTTGAATGTCTCAGTATTGATTCATGTGGTATAGTGGGGAAGTGGCTATCTCTGATGCTGCAACATGCGCCATGCCTAGAGGAGTTGTATTTGTCTTCCCGAGAGGAAGAAAATTCAGAAGAAGAAAATTCAGAAGAGGAAGAAAACAGTATATCAAATCTTAGCTCAACCAGGGAGGGCACATCATCCGGAAATCCAGATGACGGATTAGCTCTAGACCGACTGTTGCGCATACCATTAAATCTCATCTCCATTCTAAAGAGTATAACTATTGAGAGATGCCCTCATCTAACATTTAACTGGGGCAAGGAAGGCGTCTCGGGATTTACCTCCCTTGAGAAGCTAATCGTTTTGGACCGCCCCGACATGGTGCTTACAAACGGAAGATGGCTCCTCCCAAACTCACTTGGCGAACTTGAAAGCAATGACTATTCCCGAGGAACGCTGCAACCCTGCTTTCCTAGCGATATCACTAGCCTTAAAAAGTTAAAGGTACGTCGCAGCCCAGGTTTGCAATCTCTACAGCTGCACTCATGCATGGCACTGGAAGAATTGGATATTCAAGATTGTCGAAGGCTCGCTGCACTGCAGGGTCTGCAATTCCTTGGC

CTGAAAAGGCTTCACATCCAAGACCCATCTGTCCTTACCACGTCATTCTGCAGGCACCTTACCTCCCTGCAACACCTAAAACTTACTTGGTTGGAAGAAGTGAGACTAACAGATGAGCAAGAGCAAGCGCTTGTGCTCCTCAAGTCCCTGCAAGAGCTCCAATTTCATTATTGTTCCAATCTCGTAGATCTTCCTGCGGTGCTGCACAACCTTCCTTCCCTGAAGACTTTGAAGGTAGATGGGTGTAGGGGCATCTCAAGGCTGCCAGAAACAGGCCTCCCATTTTCGCTGGAAGAACTGGAAATCGAGTGGTGCAGCAAGGAGCTCGCTGATCAATGCAGGCTGCTAGCATCAAACAAG

TGAAGATACCTCTTAAGAATAAAATCTTTGCATGGTATCTTCGTCGCGGAGTCATTCTTACTAAAGATAACCTTATTAAGAGAAATTGGCATGGAAGTACGCAATGTGTATTTTGTCCGCATGATGAGACAATAAAACATTTGTTCTTCCAATGTAAATTGGCTCGTTCTATATGGTCAGTCATCCAAATAGCTTCTGGCTTGTACCCTCCTTGTAGTGTTGCTAATATATTTGGCAATTGGTTACATGGGATTGATCACAAGTTCAGAAGTCTACTTAGGGTGGGAGCGCTTGCCGTGATTTGGTCGCTTTGGCTATGTAGAAATGATAAGATTTTTAACGATAAAAGTACTTCGCTTATGCAGGTTATCTACAGATGTACTGGGACGCTTCGTTTATGGTCCTCTCTACAACGAGTGGAGAATCGAGACCTGTTTACGGAGGTGTGTACACGATTGGAGGTTACGGCGAGGGATACTTTTATCCAACATGGGTGGCGGCATGATCTTAGGATTGGGCCACCGACGGTTTAGGCGCTATACAAATATACTTTCTTTGTATTTCGCCTTCCTTTTTTATTTTTATTTTTCGCTTGTTGTGAGGATATTGTTGGCTGTGTGCATCTCAGTT

>YrSP_locus  (SEQ ID NO 7)ATGGAGCCGGCGGGAGACTCTTCCGTGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAACGCTCCTCATGGACAAGATGGAGGAGTGGATTCGGCAAGTCGGGCTTGCCGACGACGTCGAGAGGCTCCAGTCTGAGGTCGAGAGAGTCGACACGGTGGTGGCTGCTGTGAAGGGGAGGGCAGCCGGGAACAGGCCTCTGTCCCGGGCTCTCGCTCGTGTCAAGGAGCTTCTCTACGACGCCGACGACTTGATCGACGAGCTAGACTACTACAGGCTCCAACAACAAGTCGAAGGAGGTAGTAAGCATAATCCCATTATATATCGAATATATGTAAGCTCAAGATATTTATTTTGGGATGGAGGGAGTAGTTTGATCTTAATTTCTGGTCCATATTTTTTTCGGCACAGTTACGAGTGACGACCCTGACGGTATGCGTGGAGCTGAAAGAGTGGATGAAATATCAAGGGGCCATGTCGATACACTGAATTGCAGTGTTGGCAAATTACGATCCCCGGTATGGGAACACTTCACGATCACAGAAACAACTATCGACGGGAAGCGTTCAAAAGCCAAATGTAACTACTGTGGAAATGATTTTAATTGCGAAACGAAGACAAACGGGACTTCATCTATGAAAAAACATTTGGAGAAAGAGCATTCCGTGACTTGTACGAAGAAACCTGGAGCCCATCCACCAAACCCTTCAAGGTACCCAAAGGAAATTATATGTTGCATCAGCGCATTTATATTCGTTTATATATATCTGCTTGAGAGCCCATTGTTGTTCTACATTTCTTCTGATAACTGACCCACCATTTTCTCTCTTAATGCAGCACCGGCTATGCAACTGAAAATGTGACGCTTGTTGAAGTTGGTAGTTCATCCAACAGAAAAAGAAAGAGAACGAATAAGGAGCCAGCACAAACCACCGCAGATAACACCCGTTGGGACAAGGCTGAGTTATCCGATACAATAAAAAAGATTACTAGCCAGTTACAGTTACAGTTACAGGGTATCCTATGGGCTTTCAGTAAAGTTCTCGAGCCACATGGGTCTAGCTCTGCGTCGAGTTCAAATCATCACCAACCGAGTACAACCTCAGATCAGCACGCAAAAACATCAAGTCTTGCTCCAAGGAAAGTGTATGGCAGAGTAGCAGAAATGAACTCCATCAGAAATTTAATAGCAGAAAAGAAATGTGATGCTCTAACTGTTCTGCCTATTGTGGGCATTGCTGGTGTTGGAAAGACAACTCTCGCTCAATCTGTATACAATGATCCAGATATAAAAAGTCAATTTCACCACAAGATATGGGTTTGCGTGTCCCGCAAATTTGATGAAGTGATGCTCACAAGGGAGATGTTAGACTTTGAAAGACACGAGGGATCTCCTCATGAAAATGGAAGGCATGAAGGAATTAGTAGCCTTGCTAAGCTTCAGGAGATCTTGAAGGACATTATCGAGTACCAGTCAAAGAGTTTTCTGCTTATTTTAGATGATGTATGGGACAGTATGGATGATCATCAATGGAGAAAACTGGTGTGTCCTTTTGTATCAAGTCAAGCAAAGGGTAATTTAATTCTAGTCACAACCAGAAATTTGTCAGTTGCACACATGTTAGGAACACGTGAGCCGATAAAGTTGGGTGCTTTGGAAAATGATGTTATGTGGTTGCTGCTCAAGTCATGTGCATTTCGTGATGTGAATTATGAAGGGAACCAAAGTCTAAGCATTGTCGGGAGGCAAATATCAGAGAAGTTAAAGGGAAACCCACTAGCAGCAGAAACAGCGGGGGCACTATTAAGGAAGAAATTTAGCATTGATTATTGGAAAATCATTTTAAAGAATGAAGAGTGGAAATCCATGGAGCTCGGTAATGGAATCATGGCTGCTCTAAAGCTTAGCTATGATCAACTTCCCTACCATTTACAACAATGTTTCTCATATTGCTCCATATTCCCCGACGGTTATCAGTTTCTTGGTGAGGAGTTGGTCGGTTTCTGGATGTCACAGGGATTTGTAAAGTGCAACAACTCTAGTCAGAGATTGGAGCAGATAGGACAGTGCTATCTGATTGATTTGGTTAACTTAGGCTTCTTTGAAGAAGTTAAAAGAGAAGAACCATATCTGGGCTGTCGAGTTATGTATGGCATATGTGGTCTCATGCATGATTTTGTGATTATGGTGTCAAGGACTGACTGTGCAAGTATAGATGGTCTGCAGCGCAACAAAATGCCTCAAACTCTACGACATTTGTCAATAGTAACTGGATCCGCGTACAAGAAAAATCAGCACGGAAACATTCCTCGTAATAATAGGTTTGAAGAAAATCTGAGAAATACAATTACATCAGTTAGCGAGTTGAGGACATTGGTGTTACTTGGGCATTATGACTTTTCCTTCTTACTATTATTCCAAGATATATTTCAAAAGGCACATAACTTACGTGTGCTGCAAATGTCTGCACCACCTGCTGATTTTCTCAAACATAGGTTTGAGGAGGTGGATGGGTCTTTCCCTCAAATTTTGAGCAAATTGTACCATCTCCAAGTATTAGACGTCGGTGCATACACTGATCGTACTATGCCTGGTTGTATTGATAATCTTGTTAGCCTGCGGCATCTTGTTGTACACAAGGGAGTGTACTCTTCCATTGCAACCATTGATAATATGCTATCATTTCAGGAACAACATGGTTTCAAGTTTCATATTTCTAGTGGCTTTGAGATAACACGACTCCAATCCACTGAACATTGGATGCATGTTGATACTCTGGAAGATGTTTATGAGGCAGGACTGGTAAACAATGAACTCTCAGAAAAGTTGCACCTGTCCTGGAAGATTCTCCTGAGGACATAGGCATGGAGGTTGAGGATTGGGAACCACATTGGGACTTAAGGGTTCTCGAGATATCTGGGTATAATTTTGGTTCGCCAATTGTGGTTGACATCATTATCTTGGTTACATCCTCCCAGACGGTTGAGATATCCAATTGTAGTGAATGGAAAATACTTCCATCTTTGGAAAGATTTCAGTTTTTGACAAATCTGGAGTTGAGAAACCTGCCCAAAGTAATAGAAATACTGGTTCCTTCACTGGAGGAGCTAGCATTAGTTACAATGCCAAAGTTGAAGAAATGTTCATGCACTCCCGTGGAAGGTATGAGCTCTAGACTAAGAGCACTGCGGATCGAGGATTGTCAATCACTGAAGGAGTTTGATCTGTTTGAGAACAATGATAAATTCGAAACTGGGCAGAGGTCATGGGCTCCTAGTCTTAGGGAACTAAGTCTGGAGAATTGCCCCCATTTGAAAGTGTTGAAGCCTCTTCCACTCTCACTCATGTGTTCTGAGTTACTCATAAGTGGAGTTTCAACACTTCCGTACATGAAGGGGTCATCTGATAGAAAGTTATGTATTGGGTATGATGATAAGTATGACTACTATGGTTTTGACGAATCTTCCGATGAGTTGAAGATACTGGATGACAAAATTTTTATGTTCCATAATCTGAAAAACCTCAAATCAATGGTGATATATGGTTGCCGGAATCTAAGTTCCATTTCGTTAAAAGGTTTTAGTTACCTCATCTCTTTAACGAGCTTGGAAATAAGAGACTGTGAAAAACTTTTTGCTTCAGATGAGATGCCAGAGCATACCCTTGAAGATGTGACACCTGCGAATTGCAAGGCTTTCCCATCTCTTGAATGTCTCAGTATTGATTCATGTGGTATAGTGGGGAAGTGGCTATCTCTGATGCTGCAACATGCGCCATGCCTAGAGGAGTTGTATTTGTCTTCCCGAGAGGAAGAAAATTCAGAAGAAGAAAATTCAGAAGAGGAAGAAAACAGTATATCAAATCTTAGCTCAACCAGGGAGGGCACATCATCCGGAAATCCAGATGACGGATTAGCTCTAGACCGACTGTTGCGCATACCATTAAATCTCATCTCCATTCTAAAGAGTATAACTATTGAGAGATGCCCTCATCTAACATTTAACTGGGGCAAGGAAGGCGTCTCGGGATTTACCTCCCTTGAGAAGCTAATCGTTTTGGACCGCCCCGACATGGTGCTTACAAACGGAAGATGGCTCCTCCCAAACTCACTTGGCGAACTTGAAAGCAATGACTATTCCCGAGGAACGCTGCAACCCTGCTTTCCTAGCGATATCACTAGCCTTAAAAAGTTAAAGGTACGTCGCAGCCCAGGTTTGCAATCTCTACAGCTGCACTCATGCATGGCACTGGAAGAATTGGATATTCAAGATTGTCGAAGGCTCGCTGCACTGCAGGGTCTGCAATTCCTTGGCA

TGAAAAGGCTTCACATCCAAGACCCATCTGTCCTTACCACGTCATTCTGCAGGCACCTTACCTCCCTGCAACACCTAAAACTTACTTGGTTGGAAGAAGTGAGACTAACAGATGAGCAAGAGCAAGCGCTTGTGCTCCTCAAGTCCCTGCAAGAGCTCCAATTTCATTATTGTTCCAATCTCGTAGATCTTCCTGCGGTGCTGCACAACCTTCCTTCCCTGAAGACTTTGAAGGTAGATGGGTGTAGGGGCATCTCAAGGCTGCCAGAAACAGGCCTCCCATTTTCGCTGGAAGAACTGGAAATCGAGTGGTGCAGCAAGGAGCTCGCTGATCAATGCAGGCTGCTAGCATCAAACAAGC

GGCAATCTTGTGCG >Yr.5_CDSATGGAGCCGGCGGGAGACTCTTCCGTGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAACGCTCCTCATGGACAAGATGGAGGAGTGGATTCGGCAAGTCGGGCTTGCCGACGACGTCGAGAGGCTCCAGTCTGAGGTCGAGAGAGTCGACACGGTGGTGGCTGCTGTGAAGGGGAGGGCAGCCGGGAACAGGCCTCTGTCCCGGGCTCTCGCTCGTGTCAAGGAGCTTCTCTACGACGCCGACGACTTGATCGACGAGCTAGACTACTACAGGCTCCAACAACAAGTCGAAGGAGTTACGAGTGACGACCCTGACGGTATGCGTGGAGCTGAAAGAGTGGATGAAATATCAAGGGGCCATGTCGATACACTGAATTGCAGTGTTGGCAAATTACGATCCCCGGTATGGGAACACTTCACGATCACAGAAACAACTATCGACGGGAAGCGTTCAAAAGCCAAATGTAACTACTGTGGAAATGATTTTAATTGCGAAACGAAGACAAACGGGACTTCATCTATGAAAAAACATTTGGAGAAAGAGCATTCCGTGACTTGTACGAAGAAACCTGGAGCCCATCCACCAAACCCTTCAAGCACCGGCTATGCAACTGAAAATGTGACGCTTGTTGAAGTTGGTAGTTCATCCAACAGAAAAAGAAAGAGAACGAATAAGGAGCCAGCACAAACCACCGCAGATAACACCCGTTGGGACAAGGCTGAGTTATCCGATACAATAAAAAAGATTACTAGCCAGTTACAGTTACAGTTACAGGGTATCCTATGGGCTTTCAGTAAAGTTCTCGAGCCACATGGGTCTAGCTCTGCGTCGAGTTCAAATCATCACCAACCGAGTACAACCTCAGATCAGCACGCAAAAACATCAAGTCTTGCTCCAAGGAAAGTGTATGGCAGAGTAGCAGAAATGAACTCCATCAGAAATTTAATAGCAGAAAAGAAATGTGATGCTCTAACTGTTCTGCCTATTGTGGGCATTGCTGGTGTTGGAAAGACAACTCTCGCTCAATCTGTATACAATGATCCAGATATAAAAAGTCAATTTCACCACAAGATATGGGTTTGCGTGTCCCGCAAATTTGATGAAGTGATGCTCACAAGGGAGATGTTAGACTTTGAAAGACACGAGGGATCTCCTCATGAAAATGGAAGGCATGAAGGAATTAGTAGCCTTGCTAAGCTTCAGGAGATCTTGAAGGACATTATCGAGTACCAGTCAAAGAGTTTTCTGCTTATTTTAGATGATGTATGGGACAGTATGGATGATCATCAATGGAGAAAACTGGTGTGTCCTTTTGTATCAAGTCAAGCAAAGGGTAATTTAATTCTAGTCACAACCAGAAATTTGTCAGTTGCACACATGTTAGGAACACGTGAGCCGATAAAGTTGGGTGCTTTGGAAAATGATGTTATGTGGTTGCTGCTCAAGTCATGTGCATTTCGTGATGTGAATTATGAAGGGAACCAAAGTCTAAGCATTGTCGGGAGGCAAATATCAGAGAAGTTAAAGGGAAACCCACTAGCAGCAGAAACAGCGGGGGCACTATTAAGGAAGAAATTTAGCATTGATTATTGGAAAATCATTTTAAAGAATGAAGACTGGAAATCCATGGAGCTCGGTAATGGAATCATGGCTGCTCTAAAGCTTAGCTATGATCAACTTCCCTACCATTTACAACAATGTTTCTCATATTGCTCCATATTCCCCGACGGTTATCAGTTTCTTGGTGAGGAGTTGGTCGGTTTCTGGATGTCACAGGGATTTGTAAAGTGCAACAACTCTAGTCAGAGATTGGAGCAGATAGGACAGTGCTATCTGATTGATTTGGTTAACTTAGGCTTCTTTGAAGAAGTTAAAAGAGAAGAACCATATCTGGGCTGTCGAGTTATGTATGGCATATGTGGTCTCATGCATGATTTTGTGATTATGGTGTCAAGGACTGACTGTGCAAGTATAGATGGTCTGCAGCGCAACAAAATGCCTCAAACTCTACGACATTTGTCAATAGTAACTGGATCCGCGTACAAGAAAAATCAGCACGGAAACATTCCTCGTAATAATAGGTTTGAAGAAAATCTGAGAAATACAATTACATCAGTTAGCGAGTTGAGGACATTGGTGTTACTTGGGCATTATGACTTTTCCTTCTTACTATTATTCCAAGATATATTTCAAAAGGCACATAACTTACGTGTGCTGCAAATGTCTGCAGCACCTGCTGATTTTCTCAAACATAGGTTTGAGGAGGTGGATGGGTCTTTCCCTCAAATTTTGAGCAAATTGTACCATCTCCAAGTATTAGACGTCGGTGCATACACTGATCGTACTATGCCTGGTTGTATTGATAATCTTGTTAGCCTGCGGCATCTTGTTGTACACAAGGGAGTGTACTCTTCCATTGCAACCATTGATAATATGCTATCATTTCAGGAACAACATGGTTTCAAGTTTCATATTTCTAGTGGCTTTGAGATAACACGACTCCAATCCACTGAACATTGGATGCATGTTGATACTCTGGAAGATGTTTATGAGGCAGGACTGGTAAACAATGAACTCTCAGAAAAGTTGCACCTGTCCTGGAAGGATTCTCCTGAGGACATAGGCATGGAGGTTGAGGATTGGGAACCACATTGGGACTTAAGGGTTCTCGAGATATCTGGGTATAATTTTGGTTCGCCAATTGTGGTTGACATCATTATCTTGGTTACATCCTCCCAGACGGTTGAGATATCCAATTGTAGTGAATGGAAAATACTTCCATCTTTGGAAAGATTTCAGTTTTTGACAAATCTGGAGTTGAGAAACCTGCCCAAAGTAATAGAAATACTGGTTCCTTCACTGGAGGAGCTAGCATTAGTTACAATGCCAAAGTTGAAGAAATGTTCATGCACTCCCGTGGAAGGTATGAGCTCTAGACTAAGAGCACTGCGGATCGAGGATTGTCAATCACTGAAGGAGTTTGATCTGTTTGAGAACAATGATAAATTCGAAACTGGGCAGAGGTCATGGGCTCCTAGTCTTAGGGAACTAAGTCTGGAGAATTGCCCCCATTTGAAAGTGTTGAAGCCTCTTCCACTCTCACTCATGTGTTCTGAGTTACTCATAAGTGGAGTTTCAACACTTCCGTACATGAAGGGGTCATCTGATAGAAAGTTATGTATTGGGTATGATGATAAGTATGACTACTATGGTTTTGACGAATCTTCCGATGAGTTGAAGATACTGGATGACAAAATTTTTATGTTCCATAATCTGAAAAACCTCAAATCAATGGTGATATATGGTTGCCGGAATCTAAGTTCCArrrCGTTAAAAGGrrTTAGTTACCTCATCTCTTTAACGAGCTTGGAAATAAGAGACTGTGAAAAACTTTTTGCTTCAGATGAGATGCCAGAGCATACCCTTGAAGATGTGACACCTGCGAATTGCAAGGCTTTCCCATCTCTTGAATGTCTCAGTATTGATTCATGTGGTATAGTGGGGAAGTGGCTATCTCTGATGCTGCAACATGCGCCATGCCTAGAGGAGTTGTATTTGTCTTCCCGAGAGGAAGAAAATTCAGAAGAAGAAAATTCAGAAGAGGAAGAAAACAGTATATCAAATCTTAGCTCAACCAGGGAGGGCACATCATCCGGAAATCCAGATGACGGATTAGCTCTAGACCGACTGTTGCGCATACCATTAAATCTCATCTCCATTCTAAAGAGTATAACTATTGAGAGATGCCCTCATCTAACATTTAACTGGGGCAAGGAAGGCGTCTCGGGATTTACCTCCCTTGAGAAGCTAATCGTTTTGGACCGCCCCGACATGGTGCTTACAAACGGAAGATGGCTCCTCCCAAACTCACTTGGCGAACTTGAAAGCAATGACTATTCCCGAGGAACGCTGCAACCCTGCTTTCCTAGCGATATCACTAGCCTTAAAAAGTTAAAGGTACGTCGCAGCCCAGGTTTGCAATCTCTACAGCTGCACTCATGCATGGCACTGGAAGAATTGGATATTCAAGATTGTCGAAGGCTCGCTGCACTGCAGGGTCTGCAATTCCTTGGCAGCCTCACGCATTTGACCATATACAACTGCCCTGGCTTGCCACCATTTCTGGAGAGCTTTTCAAGGCAGGGCTATACGCTGTTACCTCGGCTGAAAAGGCTTCACATCCAAGACCCATCTGTCCTTACCACGTCATTCTGCAGGCACCTTACCTCCCTGCAACACCTAAAACTTACTTGGTTGGAAGAAGTGAGACTAACAGATGAGCAAGAGCAAGCGCTTGTGCTCCTCAAGTCCCTGCAAGAGCTCCAATTTCATTATTGTTCCAATCTCGTAGATCTTCCTGCGGTGCTGCACAACCTTCCTTCCCTGAAGACTTTGAAGGTAGATGGGTGTAGGGGCATCTCAAGGCTGCCAGAAACAGGCCTCCCATTTTCGCTGGAAGAACTGGAAATCGAGTGGTGCAGCAAGGAGCTCGCTGATCAATGCAGGCTGCTAGCATCAAACAAGCTAAATATCAAAATTCTCAGTGGAATCTATGTATAG >YrSP_CDSATGGAGCCGGCGGGAGACTCTTCCGTGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAACGCTCCTCATGGACAAGATGGAGGAGTGGATTCGGCAAGTCGGGCTTGCCGACGACGTCGAGAGGCTCCAGTCTGAGGTCGAGAGAGTCGACACGGTGGTGGCTGCTGTGAAGGGGAGGGCAGCCGGGAACAGGCCTCTGTCCCGGGCTCTCGCTCGTGTCAAGGAGCTTCTCTACGACGCCGACGACTTGATCGACGAGCTAGACTACTACAGGCTCCAACAACAAGTCGAAGGAGTTACGAGTGACGACCCTGACGGTATGCGTGGAGCTGAAAGAGTGGATGAAATATCAAGGGGCCATGTCGATACACTGAATTGCAGTGTTGGCAAATTACGATCCCCGGTATGGGAACACTTCACGATCACAGAAACAACTATCGACGGGAAGCGTTCAAAAGCCAAATGTAACTACTGTGGAAATGATTTTAATTGCGAAACGAAGACAAACGGGACTTCATCTATGAAAAAACATTTGGAGAAAGAGCATTCCGTGACTTGTACGAAGAAACCTGGAGCCCATCCACCAAACCCTTCAAGCACCGGCTATGCAACTGAAAATGTGACGCTTGTTGAAGTTGGTAGTTCATCCAACAGAAAAAGAAAGAGAACGAATAAGGAGCCAGCACAAACCACCGCAGATAACACCCGTTGGGACAAGGCTGAGTTATCCGATACAATAAAAAAGATTACTAGCCAGTTACAGTTACAGTTACAGGGTATCCTATGGGCTTTCAGTAAAGTTCTCGAGCCACATGGGTCTAGCTCTGCGTCGAGTTCAAATCATCACCAACCGAGTACAACCTCAGATCAGCACGCAAAAACATCAAGTCTTGCTCCAAGGAAAGTGTATGGCAGAGTAGCAGAAATGAACTCCATCAGAAATTTAATAGCAGAAAAGAAATGTGATGCTCTAACTGTTCTGCCTATTGTGGGCATTGCTGGTGTTGGAAAGACAACTCTCGCTCAATCTGTATACAATGATCCAGATATAAAAAGTCAATTTCACCACAAGATATGGGTTTGCGTGTCCCGCAAATTTGATGAAGTGATGCTCACAAGGGAGATGTTAGACTTTGAAAGACACGAGGGATCTCCTCATGAAAATGGAAGGCATGAAGGAATTAGTAGCCTTGCTAAGCTTCAGGAGATCTTGAAGGACATTATCGAGTACCAGTCAAAGAGTTTTCTGCTTATTTTAGATGATGTATGGGACAGTATGGATGATCATCAATGGAGAAAACTGGTGTGTCCTTTTGTATCAAGTCAAGCAAAGGGTAATTTAATTCTAGTCACAACCAGAAATTTGTCAGTTGCACACATGTTAGGAACACGTGAGCCGATAAAGTTGGGTGCTTTGGAAAATGATGTTATGTGGTTGCTGCTCAAGTCATGTGCATTTCGTGATGTGAATTATGAAGGGAACCAAAGTCTAAGCATTGTCGGGAGGCAAATATCAGAGAAGTTAAAGGGAAACCCACTAGCAGCAGAAACAGCGGGGGCACTATTAAGGAAGAAATTTAGCATTGATTATTGGAAAATCATTTTAAAGAATGAAGACTGGAAATCCATGGAGCTCGGTAATGGAATCATGGCTGCTCTAAAGCTTAGCTATGATCAACTTCCCTACCATTTACAACAATGTTTCTCATATTGCTCCATATTCCCCGACGGTTATCAGTTTCTTGGTGAGGAGTTGGTCGGTTTCTGGATGTCACAGGGATTTGTAAAGTGCAACAACTCTAGTCAGAGATTGGAGCAGATAGGACAGTGCTATCTGATTGATTTGGTTAACTTAGGCTTCTTTGAAGAAGTTAAAAGAGAAGAACCATATCTGGGCTGTCGAGTTATGTATGGCATATGTGGTCTCATGCATGATTTTGTGATTATGGTGTCAAGGACTGACTGTGCAAGTATAGATGGTCTGCAGCGCAACAAAATGCCTCAAACTCTACGACATTTGTCAATAGTAACTGGATCCGCGTACAAGAAAAATCAGCACGGAAACATTCCTCGTAATAATAGGTTTGAAGAAAATCTGAGAAATACAATTACATCAGTTAGCGAGTTGAGGACATTGGTGTTACTTGGGCATTATGACTTTTCCTTCTTACTATTATTCCAAGATATATTTCAAAAGGCACATAACTTACGTGTGCTGCAAATGTCTGCACCACCTGCTGATTTTCTCAAACATAGGTTTGAGGAGGTGGATGGGTCTTTCCCTCAAATTTTGAGCAAATTGTACCATCTCCAAGTATTAGACGTCGGTGCATACACTGATCGTACTATGCCTGGTTGTATTGATAATCTTGTTAGCCTGCGGCATCTTGTTGTACACAAGGGAGTGTACTCTTCCATTGCAACCATTGATAATATGCTATCATTTCAGGAACAACATGGTTTCAAGTTTCATATTTCTAGTGGCTTTGAGATAACACGACTCCAATCCACTGAACATTGGATGCATGTTGATACTCTGGAAGATGTTTATGAGGCAGGACTGGTAAACAATGAACTCTCAGAAAAGTTGCACCTGTCCTGGAAGATTCTCCTGAGGACATAG >Yr5_protein  (SEQ ID NO: 2)MEPAGDSSVEAAIAWLVQTILATLLMDKMEEWIRQVGLADDVERLQSEVERVDTVVAAVKGRAAGNRPLSRALARVKELLYDADDLIDELDYYRLQQQVEGVTSDDPDGMRGAERVDEISRGHVDTLNCSVGKLRSPVWEHFTITETTIDGKRSKAKCNYCGNDFNCETKTNGTSSMKKHLEKEHSVTCTKKPGAHPPNPSSTGYATENVTLVEVGSSSNRKRKRTNKEPAQTTADNTRWDKAELSDTIKKITSQLQLQLQGILWAFSKVLEPHGSSSASSSNHHQPSTTSDQHAKTSSLAPRKVYGRVAEMNSIRNLIAEKKCDALTVLPIVGIAGVGKTTLAQSVYNDPDIKSQFHHKIWVCVSRKFDEVMLTREMLDFERHEGSPHENGRHEGISSLAKLQEILKDIIEYQSKSFLLILDDVWDSMDDHQWRKLVCPFVSSQAKGNLILVTTRNLSVAHMLGTREPIKLGALENDVMWLLLKSCAFRDVNYEGNQSLSIVGRQISEKLKGNPLAAETAGALLRKKFSIDYWKIILKNEDWKSMELGNGIMAALKLSYDQLPYHLQQCFSYCSIFPDGYQFLGEELVGFWMSQGFVKCNNSSQRLEQIGQCYLIDLVNLGFFEEVKREEPYLGCRVMYGICGLMHDFVIMVSRTDCASIDGLQRNKMPQTLRHLSIVTGSAYKKNQHGNIPRNNRFEENLRNTITSVSELRTLVLLGHYDFSFLLLFQDIFQKAHNLRVLQMSAAPADFLKHRFEEVDGSFPQILSKLYHLQVLDVGAYTDRTMPGCIDNLVSLRHLVVHKGVYSSIATIDNMLSFQEQHGFKFHISSGFEITRLQSTEHWMHVDTLEDVYEAGLVNNELSEKLHLSWKDSPEDIGMEVEDWEPHWDLRVLEISGYNFGSPIVVDIIILVTSSQTVEISNCSEWKILPSLERFQFLTNLELRNLPKVIEILVPSLEELALVTMPKLKKCSCTPVEGMSSRLRALRIEDCQSLKEFDLFENNDKFETGQRSWAPSLRELSLENCPHLKVLKPLPLSLMCSELLISGVSTLPYMKGSSDRKLCIGYDDKYDYYGFDESSDELKILDDKIFMFHNLKNLKSMVTYGCRNLSSISLKGFSYLISLTSLEIRDCEKLFASDEMPEHTLEDVTPANCKAFPSLECLSIDSCGIVGKWLSLMLQHAPCLEELYLSSREEENSEEENSEEEENSISNLSSTREGTSSGNPDDGLALDRLLRIPLNLISILKSITIERCPHLTFNWGKEGVSGFTSLEKLIVLDRPDMVLTNGRWLLPNSLGELESNDYSRGTLQPCFPSDITSLKKLKVRRSPGLQSLQLHSCMALEELDIQDCRRLAALQGLQFLGSLTHLTIYNCPGLPPFLESFSRQGYTLLPRLKRLHIQDPSVLTTSFCRHLTSLQHLKLTWLEEVRLTDEQEQALVLLKSLQELQFHYCSNLVDLPAVLHNLPSLKTLKVDGCRGISRLPETGLPFSLEELEIEWCSKELADQCRLLASNKLNIKILSGIYV- >YrSP_protein (SEQ ID NO: 6)MEPAGDSSVEAAIAWLVQTILATLLMDKMEEWIRQVGLADDVERLQSEVERVDTVVAAVKGRAAGNRPLSRALARVKELLYDADDLIDELDYYRLQQQVEGVTSDDPDGMRGAERVDEISRGHVDTLNCSVGKLRSPVWEHFTITETTIDGKRSKAKCNYCGNDFNCETKTNGTSSMKKHLEKEHSVTCTKKPGAHPPNPSSTGYATENVTLVEVGSSSNRKRKRTNKEPAQTTADNTRWDKAELSDTIKKITSQLQLQLQGILWAFSKVLEPHGSSSASSSNHHQPSTTSDQHAKTSSLAPRKVYGRVAEMNSIRNLIAEKKCDALTVLPIVGIAGVGKTTLAQSVYNDPDIKSQFHHKIWVCVSRKFDEVMLTREMLDFERHEGSPHENGRHEGISSLAKLQEILKDIIEYQSKSFLLILDDVWDSMDDHQWRKLVCPFVSSQAKGNLILVTTRNLSVAHMLGTREPIKLGALENDVMWLLLKSCAFRDVNYEGNQSLSIVGRQISEKLKGNPLAAETAGALLRKKFSIDYWKIILKN EDWKSMELGNGIMAALKLSYDQLPYHLQQCFSYCSIFPDGYQFLGEELVGFWMSQGFVKCNNSSQRLEQrGQCYLIDLVNLGFFEEVKREEPYLGCRVMYGICGLMHDFVIMVSRTDCASIDGLQRNKMPQTLRHLSIVTGSAYKKNQHGNIPRNNRFEENLRNTITSVSELRTLVLLGHYDFSFLLLFQDIFQKAHNLRVLQMSAPPADFLKHRFEEVDGSFPQILSKLYHLQVLDVGAYTDRTMPGCIDNLVSLRHLVVHKGVYSSIATIDNMLSFQEQHGFKFHISSGFEITRLQSTEHWMHVDTLEDVYEAGLVNNELSEKLHLSWKILLRT- >Yr7_with_NsATGGAGCCGGCGGGAGACTCTTCCCTGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAACGCTCCTCATGGACAAGATGGAGGCCTGGATTCAGCAAGTCGGGCTTGCCGACGACGTCGAGAGGCTCCAGTCTGAGGTCGAGAGAGTCGACACGGTGGTGGCTGCTGTGAAGGGGAGGGCAGCCGGGAACATGCCTCTGTCCCGGTCTCTCGCTCGTGTCAAGGAGCTTCTCTATGACGCCGACGACGTGATCGACGAGCTAGACTACTACAGGCTCCAACACCAAGTCGAAGGAGGTAGTAAGCATAATCCCATTATATATCGAATCTATGTGTGCTACTCAATAGTTTGATCTTAATTTCTGGTCCATGTTTCTTTTCGGCACAGTTACAAGTGACGAGCCTGACGGTATGCGTGGAGCTGAAAGAGTGGATGAAATATCAAGGGGCCATGTCGATACACTGAATGTCAGTGTTGGCAAATTACGGTCCCCGGTATGGGAACACTTCACCATCACAGAAACAACTATCGACGGGAAGCGTTCAAAAGCCAAATGTAAGTACTGTGGAAATGATTTTAATTGCGAAACGAAGACAAACGGGACTTCATCTATGAAAAAACATTTGGAGAAGGAGCATTCCGTGACTTGCACGAATAAATCTGCAGTGCACCCCCCAAACACTTCAAGGTACCAGCAGGAATTTATACCTTGCTTCAACGAATTTGTTGTAATTGTTTATATACGTCTGCTTGAGAGCCCATTGTTGTTCTGAATTTCTTCTGATAACCAACCCACCATCCTTTTCTTACTGCAGCACCGGCGATGCTACTTGTAATGTGAGGTCGGTTGAAGTTGGTAGTTCGTCCAACGGAAAAAGAAAGAGAACAAATGAGGATCCNAAGGCTGAGTTATCCAATAGGATAATTAAAATTACTGAGAAGTTACAGTTACAGGACATCCAGGGGGCTTTGAGTAAAGTTCTCGAGCCATATGGATCCAGCGCTACTTCAAGTTCAAATCATCACCGCTTGAGTACAGCATCAGATCAGCACCCAACAACATCAAGTCTTGTTCCAATGGAAGTTTATGGCAGAGTTGCAGAAAAGAATAAGATCAAAAAGTCAATAACTGAAAACCAATCTGGTGGTGTAAATGTTCTGCCTATTGTAGGCATTGCAGGTGTTGGAAAGACAACTCTTGCTCAATTTGTGTATAATGATCCAGACNCAGAAAAGAATAAGATCAAAAAGTCAATAACTGAAAACCAATCTGGTGGTGTAAATGTTCTGCCTATTGTAGGCATTGCAGGTGTTGGAAAGACAACTCTTGCTCAATTTGTGTATAATGATCCAGACGTGAAAAGTCAATTTCACCACAGGATATGGGTTTGTGTGTCCTGCAAATTTGATGAAGTGAAGCTCACAAAGGAGATGTTAGACTTTTTTCCTCGAGAAAGGCATGAAGGAATTAACAACTTCGCGAAGCTTCAAGAGATCTTGAAAGAACATGTCGAGTACCAAGCAAAGAGTTTTCTGCTCATTTTAGATGATGTCTCGGACAGTATGGATTATCATAAATGGAACAAATTGTTGAACCCTTTGCTATCAAGTCAAGCGAAGAATATAATTCTAGTCACGACCAGAAATTTGTCTGTTGCACAAAGGTTAAGCACACTTGAACCGATCAAGTTAGGTGCTTTAGAAAACGATGATATGTGGTTATTGCTCAAGTCATGTGCATTTGGTTTTGGGAACTATGAAGGTACGGAAAATCTAAGCACTATTGGAAGACAAATAGCAGAGAAGTTAAAGGGCAATCCGTTAGCAGCAGTAACTGCAGGGGCACTGTTAAGAGATAATCTTAGCATTGATCATTGGAGTAACATTCTCAAGAATGAGAAGTGGAAATCGCTGGGACTCAGTGGGGGCATCATGCCTGCTTTGAAGCTTAGTTATGATGAGTTGACGTACCGTTTACAACAATGTTTCTCGTATTGCTCTATATTTCCTGACAAATATAGGTTTCTCGGGAAGGATTTGGTCTATATTTGGATTTCTCAGGGATTTGTGAATTGCACCCAAAATAAGAGATTGGAGGAGACGGGATGGGAATATCTGAATCAATTGGTAAATCTTGGATTCTTTCAACAAATTGAAGAACAACAAGAATTGGATGGGGAAGAAGAATTCTCTCTACGCCGTCAGATTTGGTACTCTATGTGTGATCTCATGCATGATTTCGCAAGGATGATTTCAAGGACTGAATGTGCGACTATAGATGGTCTACAGTGCAATAAAATATTCCCAACTGTACAGCATTTGTCAATAGTAACCGGTTCTGCATACAACAAAGATCTGAAGGGGAACATTCCTCGTAATGAGAAGTTTGAAGAAAATATGAGAAATTCAGTTACATCAGTTACCAAATTGAGAACATTGGTTGTGCTTGGGAACTTTGACTCTTTCTTTGTACGGTTGTTCCAAGATATATTCCAGAAGGCACAAAATTTACGCCTGCTGCTAGTATCTCTAGCATCCACTTATCTGTCTCAAGTGCCTGCTGCATTCAATGATTTTAATTCCTTCCTGTGCAATTTGGCAAATCCTTTGCATCTTCGTTACCTAAAACTTGAGTTGGATGGGATTGTGCCACAAGTTTTGAGTACGTTTTTTCATCTTCAAGTATTAGATGTTGGATCAAGCATGGATACTTCTCTACCCAATGGCTTGTTGCATAATCTTGTTAGCCTGCGACATCTTGTTGCACACAAGAGAGTCCATTCTTCCATTACTAGCATTGGTAACATGACATCTATCCAGGAGCTACATGATTTTGAAGTTCGAATTTCTAGCGGCTTTGAGATAACACGACTCCAATCCATGAACGAGCTTGTTCAACTTGGGTTGTCTCAACTTGACAGTGTTAAAACCAGGGAGGACGCTTATGGGGCAGGACTAAGAAACAAGGAACACTTAGAAGAGCTTCATTTGTCCTGGAAGGATGCATATTCAGAGTATGAGTATGCCAGTGACACTGAATTTGAATCTTCTGCAAACATGGCAAGAGAAGTGATTGAGGGTCTTGAACCACACATGGATTTAAAACATCTACAAATATCTCAGTATAATGGTACCACTTCACCAGCTTGGCTTGCCAACAATATCTCAGTTACCTCATTGCAGACGCTTCATCTTGATGATTGTGGAGGATGGAGAATACTTCCATCTCTGGGAAGTCTTCCATTCCTTACAAAGGTGAAGTTGAGCAGCATGCTGGAAGTAATTGAAGTACTGATTCCTTCACTGGAGGAGCTAGTTCTAATTAAAATGCCGAAGTTAGTGAGATGCTCAAGCACTTCTGCCGAGGGTCTGAGCTCTAGCTTAAGGGTACTGCACATTGAGGATTGTGAAGCATTGAAGGAGTTTGATCTGTTTGAGAACGATTATAATTCTGAAATCATTCAGGGATCATGGCTGCCTGGTCTTAGGAATTTGATTCTATATTGTTGCCCTCATTTGAAAGTGTTGAAGCCTCTTCCACCTTCAACTACCTTTTCTAAGGTACTCATCAGAGAAATTTCAAGATTTCCGTCTATGGAGGTATCATCTGGTGAGAAGTTACAAATTGGGAATATTGATGTGTACATAGGCGATGATTTTGATGAGTCTTCTGATGAGTTGAGCATACTGGATGACAAAACTTTGGCGTTCCATAATCTTAGAAACCTGAAATCGATGGAGATATATGGTTGCAGAAATCTAAGGTCTTTTTCGTTCGAAGGTTTCAGTCATCTTGTCTCTTTAACAAGTTTGAAAATAGTAAGCTGTGAACAACTTTTCCCTTCAGATGTGACGGCAGAGTATACCCTTGAAGATGTGACAGCTGTGAACTGCAATGCCTTCCCATATCTTAAAAGCCTCAGTATCGACTCATGTGGAATAGCGGGGAAGTGGCTATCGCTGATGCTGCAGCATGCGCCAGGCCTAGAGGAATTGAGTTTAACAAGTTGCGCCCATATAACAAGAGTAGTGTTACCGATGGAAGAGGAAGAAAACAATCTATTAACAACAGTACTGTCATCAGGAAATCAAGATGAGGCATTGACATGGTTAGTTCGTGACGGACTCTTGCACATTCCATCAAATCTCGTCTCCTCTCTCAAGAATATGAGTATTACTCAGTGCCCTCGCCTAAAGTTTAACTCAGGCAAGGACTGCTTCTCTGGATTTACCTCGCTTGAGAAGCTTGAAATTTGGGGATCGTTGGTGGATGATGACGGAAGTGATGACCTGGAGAATGGAAGTTCTTTTGTGTTCGGAGAGGAGGATCAACCCCTGGGGGCGAACGGAAGATGGCTCCTCCCGACATCACTTCAGGAACTTCACATCGTGTCATTGTATTGCCAAGAAACGCTGCAAGTCTGCTTCCCTAGAGATATCACCAGCCTTAAAAAGTTAAGTGTACGTTCCGGCCAAGGTTTGCAATCTCTACAGCTGTACTCATGCACGGCACTGGAAGAATTGGCAATTTCCGGCTCTGGATCGGTCACCGTCACTGTACTAGAGGGCACGCAACCCGCTGGCAGCCTCGGGCGTTTGAATGTATCAGACTGTCCTGGCTTGCCATCACGTTTGGACAGCTTTCCAAGGTTGTGCCCTCGGCTGGAAAGGCTTGACATCAATGACCCATCTGTCCTTACCACGCCATTCTGCAAGCACCTCACCTCCCTGCAACGCCTAAAACTTGGCTTCTTGAAAGTGACGAGACTAACAGATGAGCAAGAACGAGCGCTTGTGCTCCTCAAGTCACTGAAAGAGCTCGAGATTTTTTATTGTACTCATCTCATAGATCTTCCTGCGGGGCTGCAGACCCTTCCTTCCCTCAAGAGTTTGAAGATAGAAGAGGGTCGAGGCATCTCAAGGCTGCCGGAAGCAGGCCTCCCACATTCGCTGGAAGAACTGGAAATCAAAATTTGCAGCAAGCTAGAAGATGAATGCAGGCGGCTAGCAACATGCGAAGGCAAGCTAAAAGTCAAAATTGATGGTCGATATGTGAATTAA >curated_Yr7ATGGAGCCGGCGGGAGACTCTTCCCTGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAACGCTCCTCATGGACAAGATGGAGGCCTGGATTCAGCAAGTCGGGCTTGCCGACGACGTCGAGAGGCTCCAGTCTGAGGTCGAGAGAGTCGACACGGTGGTGGCTGCTGTGAAGGGGAGGGCAGCCGGGAACATGCCTCTGTCCCGGTCTCTCGCTCGTGTCAAGGAGCTTCTCTATGACGCCGACGACGTGATCGACGAGCTAGACTACTACAGGCTCCAACACCAAGTCGAAGGAGGTAGTAAGCATAATCCCATTATATATCGAATCTATGTGTGCTACTCAATAGTTTGATCTTAATTTCTGGTCCATGTTTCTTTTCGGCACAGTTACAAGTGACGAGCCTGACGGTATGCGTGGAGCTGAAAGAGTGGATGAAATATCAAGGGGCCATGTCGATACACTGAATGTCAGTGTTGGCAAATTACGGTCCCCGGTATGGGAACACTTCACCATCACAGAAACAACTATCGACGGGAAGCGTTCAAAAGCCAAATGTAAGTACTGTGGAAATGATTTTAATTGCGAAACGAAGACAAACGGGACTTCATCTATGAAAAAACATTTGGAGAAGGAGCATTCCGTGACTTGCACGAATAAATCTGCAGTGCACCCCCCAAACACTTCAAGGTACCAGCAGGAATTTATACCTTGCTTCAACGAATTTGTTGTAATTGTTTATATACGTCTGCTTGAGAGCCCATTGTTGTTCTGAATTTCTTCTGATAACCAACCCACCATCCTTTTCTTACTGCAGCACCGGCGATGCTACTTGTAATGTGAGGTCGGTTGAAGTTGGTAGTTCGTCCAACGGAAAAAGAAAGAGAACAAATGAGGATCCAACGCAGACCACCGCAGCTAACATACACGCCCAATGGGACAAGGCTGAGTTATCCAATAGGATAATTAAAATTACTGAGAAGTTACAGTTACAGGACATCCAGGGGGCTTTGAGTAAAGTTCTCGAGCCATATGGATCCAGCGCTACTTCAAGTTCAAATCATCACCGCTTGAGTACAGCATCAGATCAGCACCCAACAACATCAAGTCTTGTTCCAATGGAAGTTTATGGCAGAGTTGCAGAAAAGAATAAGATCAAAAAGTCAATAACTGAAAACCAATCTGGTGGTGTAAATGTTCTGCCTATTGTAGGCATTGCAGGTGTTGGAAAGACAACTCTTGCTCAATTTGTGTATAATGATCCAGACGTGAAAAGTCAATTTCACCACAGGATATGGGTTTGTGTGTCCTGCAAATTTGATGAAGTGAAGCTCACAAAGGAGATGTTAGACTTTTTTCCTCGAGAAAGGCATGAAGGAATTAACAACTTCGCGAAGCTTCAAGAGATCTTGAAAGAACATGTCGAGTACCAAGCAAAGAGTTTTCTGCTCATTTTAGATGATGTCTCGGACAGTATGGATTATCATAAATGGAACAAATTGTTGAACCCTTTGCTATCAAGTCAAGCGAAGAATATAATTCTAGTCACGACCAGAAATTTGTCTGTTGCACAAAGGTTAAGCACACTTGAACCGATCAAGTTAGGTGCTTTAGAAAACGATGATATGTGGTTATTGCTCAAGTCATGTGCATTTGGTTTTGGGAACTATGAAGGTACGGAAAATCTAAGCACTATTGGAAGACAAATAGCAGAGAAGTTAAAGGGCAATCCGTTAGCAGCAGTAACTGCAGGGGCACTGTTAAGAGATAATCTTAGCATTGATCATTGGAGTAACATTCTCAAGAATGAGAAGTGGAAATCGCTGGGACTCAGTGGGGGCATCATGCCTGCTTTGAAGCTTAGTTATGATGAGTTGACGTACCGTTTACAACAATGTTTCTCGTATTGCTCTATATTTCCTGACAAATATAGGTTTCTCGGGAAGGATTTGGTCTATATTTGGATTTCTCAGGGATTTGTGAATTGCACCCAAAATAAGAGATTGGAGGAGACGGGATGGGAATATCTGAATCAATTGGTAAATCTTGGATTCTTTCAACAAATTGAAGAACAACAAGAATTGGATGGGGAAGAAGAATTCTCTCTACGCCGTCAGATTTGGTACTCTATGTGTGATCTCATGCATGATTTCGCAAGGATGATTTCAAGGACTGAATGTGCGACTATAGATGGTCTACAGTGCAATAAAATATTCCCAACTGTACAGCATTTGTCAATAGTAACCGGTTCTGCATACAACAAAGATCTGAAGGGGAACATTCCTCGTAATGAGAAGTTTGAAGAAAATATGAGAAATTCAGTTACATCAGTTACCAAATTGAGAACATTGGTTGTGCTTGGGAACTTTGACTCTTTCTTTGTACGGTTGTTCCAAGATATATTCCAGAAGGCACAAAATTTACGCCTGCTGCTAGTATCTCTAGCATCCACTTATCTGTCTCAAGTGCCTGCTGCATTCAATGATTTTAATTCCTTCCTGTGCAATTTGGCAAATCCTTTGCATCTTCGTTACCTAAAACTTGAGTTGGATGGGATTGTGCCACAAGTTTTGAGTACGTTTTTTCATCTTCAAGTATTAGATGTTGGATCAAGCATGGATACTTCTCTACCCAATGGCTTGTTGCATAATCTTGTTAGCCTGCGACATCTTGTTGCACACAAGAGAGTCCATTCTTCCATTACTAGCATTGGTAACATGACATCTATCCAGGAGCTACATGATTTTGAAGTTCGAATTTCTAGCGGCTTTGAGATAACACGACTCCAATCCATGAACGAGCTTGTTCAACTTGGGTTGTCTCAACTTGACAGTGTTAAAACCAGGGAGGACGCTTATGGGGCAGGACTAAGAAACAAGGAACACTTAGAAGAGCTTCATTTGTCCTGGAAGGATGCATATTCAGAGTATGAGTATGCCAGTGACACTGAATTTGAATCTTCTGCAAACATGGCAAGAGAAGTGATTGAGGGTCTTGAACCACACATGGATTTAAAACATCTACAAATATCTCAGTATAATGGTACCACTTCACCAGCTTGGCTTGCCAACAATATCTCAGTTACCTCATTGCAGACGCTTCATCTTGATGATTGTGGAGGATGGAGAATACTTCCATCTCTGGGAAGTCTTCCATTCCTTACAAAGGTGAAGTTGAGCAGCATGCTGGAAGTAATTGAAGTACTGATTCCTTCACTGGAGGAGCTAGTTCTAATTAAAATGCCGAAGTTAGTGAGATGCTCAAGCACTTCTGCCGAGGGTCTGAGCTCTAGCTTAAGGGTACTGCACATTGAGGATTGTGAAGCATTGAAGGAGTTTGATCTGTTTGAGAACGATTATAATTCTGAAATCATTCAGGGATCATGGCTGCCTGGTCTTAGGAATTTGATTCTATATTGTTGCCCTCATTTGAAAGTGTTGAAGCCTCTTCCACCTTCAACTACCTTTTCTAAGGTACTCATCAGAGAAATTTCAAGATTTCCGTCTATGGAGGTATCATCTGGTGAGAAGTTACAAATTGGGAATATTGATGTGTACATAGGCGATGATTTTGATGAGTCTTCTGATGAGTTGAGCATACTGGATGACAAAACTTTGGCGTTCCATAATCTTAGAAACCTGAAATCGATGGAGATATATGGTTGCAGAAATCTAAGGTCTTTTTCGTTCGAAGGTTTCAGTCATCTTGTCTCTTTAACAAGTTTGAAAATAGTAAGCTGTGAACAACTTTTCCCTTCAGATGTGACGGCAGAGTATACCCTTGAAGATGTGACAGCTGTGAACTGCAATGCCTTCCCATATCTTAAAAGCCTCAGTATCGACTCATGTGGAATAGCGGGGAAGTGGCTATCGCTGATGCTGCAGCATGCGCCAGGCCTAGAGGAATTGAGTTTAACAAGTTGCGCCCATATAACAAGAGTAGTGTTACCGATGGAAGAGGAAGAAAACAATCTATTAACAACAGTACTGTCATCAGGAAATCAAGATGAGGCATTGACATGGTTAGTTCGTGACGGACTCTTGCACATTCCATCAAATCTCGTCTCCTCTCTCAAGAATATGAGTATTACTCAGTGCCCTCGCCTAAAGTTTAACTCAGGCAAGGACTGCTTCTCTGGATTTACCTCGCTTGAGAAGCTTGAAATTTGGGGATCGTTGGTGGATGATGACGGAAGTGATGACCTGGAGAATGGAAGTTCTTTTGTGTTCGGAGAGGAGGATCAACCCCTGGGGGCGAACGGAAGATGGCTCCTCCCGACATCACTTCAGGAACTTCACATCGTGTCATTGTATTGCCAAGAAACGCTGCAAGTCTGCTTCCCTAGAGATATCACCAGCCTTAAAAAGTTAAGTGTACGTTCCGGCCAAGGTTTGCAATCTCTACAGCTGTACTCATGCACGGCACTGGAAGAATTGGCAATTTCCGGCTCTGGATCGGTCACCGTCACTGTACTAGAGGGCACGCAACCCGCTGGCAGCCTCGGGCGTTTGAATGTATCAGACTGTCCTGGCTTGCCATCACGTTTGGACAGCTTTCCAAGGTTGTGCCCTCGGCTGGAAAGGCTTGACATCAATGACCCATCTGTCCTTACCACGCCATTCTGCAAGCACCTCACCTCCCTGCAACGCCTAAAACTTGGCTTCTTGAAAGTGACGAGACTAACAGATGAGCAAGAACGAGCGCTTGTGCTCCTCAAGTCACTGAAAGAGCTCGAGATTTTTTATTGTACTCATCTCATAGATCTTCCTGCGGGGCTGCAGACCCTTCCTTCCCTCAAGAGTTTGAAGATAGAAGAGGGTCGAGGCATCTCAAGGCTGCCGGAAGCAGGCCTCCCACATTCGCTGGAAGAACTGGAAATCAAAATTTGCAGCAAGCTAGAAGATGAATGCAGGCGGCTAGCAACATGCGAAGGCAAGCTAAAAGTCAAAATTGATGGTCGATATGTGAATTAA >Yr7_Paragon_with_NsATGGAGCCGGCGGGAGACTCTTCCCTGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAACGCTCCTCATGGACAAGATGGAGGCCTGGATTCAGCAAGTCGGGCTTGCCGACGACGTCGAGAGGCTCCAGTCTGAGGTCGAGAGAGTCGACACGGTGGTGGCTGCTGTGAAGGGGAGGGCAGCCGGGAACATGCCTCTGTCCCGGTCTCTCGCTCGTGTCAAGGAGCTTCTCTATGACGCCGACGACGTGATCGACGAGCTAGACTACTACAGGCTCCAACACCAAGTCGAAGGAGGTAGTAAGCATAATCCCATTATATATCGAATCTATGTGTGCTACTCAATAGTTTGATCTTAATTTCTGGTCCATGTTTCTTTTCGGCACAGTTACAAGTGACGAGCCTGACGGTATGCGTGGAGCTGAAAGAGTGGATGAAATATCAAGGGGCCATGTCGATACACTGAATGTCAGTGTTGGCAAATTACGGTCCCCGGTATGGGAACACTTCACCATCACAGAAACAACTATCGACGGGAAGCGTTCAAAAGCCAAATGTAAGTACTGTGGAAATGATTTTAATTGCGAAACGAAGACAAACGGGACTTCATCTATGAAAAAACATTTGGAGAAGGAGCATTCCGTGACTTGCACGAATAAATCTGCAGTGCACCCCCCAAACACTTCAAGGTACCAGCAGGAATTTATACCTTGCTTCAACGAATTTGTTGTAATTGTTTATATACGTCTGCTTGAGAGCCCATTGTTGTTCTGAATTTCTTCTGATAACCAACCCACCATCCTTTTCTTACTGCAGCACCGGCGATGCTNACGGAAAAAGAAAGAGAACAAATGAGGATCCAACGCAGACCACCGCAGCTAACATACACGCCCAATGGGACAAGGCTGAGTTATCCAATAGGATAATTAAAATTACTGAGAAGTTACAGTTACAGGACATCCAGGGGGCTTTGAGTAAAGTTCTCGAGCCATATGGATCCAGCGCTACTTCAAGTTCAAATCATCACCGCTTGAGTACAGCATCAGATCAGCACCCAACAACATCAAGTCTTGTTCCAATGGAAGTTTATGGCAGAGTTGCAGAAAAGAATAAGATCAAAAAGTCAATAACTGAAAACCAATCTGGTGGTGTAAATGNCTTGAGTACAGCATCAGATCAGCACCCAACAACATCAAGTCTTGTTCCAATGGAAGTTTATGGCAGAGTTGCAGAAAAGAATAAGATCAAAAAGTCAATAACTGAAAACCAATCTGGTGGTGTAAATGTTCTGCCTATTGTAGGCATTGCAGGTGTTGGAAAGACAACTCTTGCTCAATTTGTGTATAATGATCCAGACGTGAAAAGTCAATTTCACCACAGGATATGGGTTTGTGTGTCCTGCAAATTTGATGAAGTGAAGCTCACAAAGGAGATGTTAGACTTTTTTCCTCGAGAAAGGCATGAAGGAATTAACAACTTCGCGAAGCTTCAAGAGATCTTGAAAGAACATGTCGAGTACCAAGCAAAGAGTTTTCTGCTCATTTTAGATGATGTCTCGGACAGTATGGATTATCATAAATGGAACAAATTGTTGAACCCTTTGCTATCAAGTCAAGCGAAGAATATAATTCTAGTCACGACCAGAAATTTGTCTGTTGCACAAAGGTTAAGCACACTTGAACCGATCAAGTTAGGTGCTTTAGAAAACGATGATATGTGGTTATTGCTCAAGTCATGTGCATTTGGTTTTGGGAACTATGAAGGTACGGAAAATCTAAGCACTATTGGAAGACAAATAGCAGAGAAGTTAAAGGGCAATCCGTTAGCAGCAGTAACTGCAGGGGCACTGTTAAGAGATAATCTTAGCATTGATCATTGGAGTAACATTCTCAAGAATGAGAAGTGGAAATCGCTGGGACTCAGTGGGGGCATCATGCCTGCTTTGAAGCTTAGTTATGATGAGTTGACGTACCGTTTACAACAATGTTTCTCGTATTGCTCTATATTTCCTGACAAATATAGGTTTCTCGGGAAGGATTTGGTCTATATTTGGATTTCTCAGGGATTTGTGAATTGCACCCAAAATAAGAGATTGGAGGAGACGGGATGGGAATATCTGAATCAATTGGTAAATCTTGGATTCTTTCAACAAATTGAAGAACAACAAGAATTGGATGGGGAAGAAGAATTCTCTCTACGCCGTCAGATTTGGTACTCTATGTGTGATCTCATGCATGATTTCGCAAGGATGATTTCAAGGACTGAATGTGCGACTATAGATGGTCTACAGTGCAATAAAATATTCCCAACTGTACAGCATTTGTCAATAGTAACCGGTTCTGCATACAACAAAGATCTGAAGGGGAACATTCCTCGTAATGAGAAGTTTGAAGAAAATATGAGAAATTCAGTTACATCAGTTACCAAATTGAGAACATTGGTTGTGCTTGGGAACTTTGACTCTTTCTTTGTACGGTTGTTCCAAGATATATTCCAGAAGGCACAAAATTTACGCCTGCTGCTAGTATCTCTAGCATCCACTTATCTGTCTCAAGTGCCTGCTGCATTCAATGATTTTAATTCCTTCCTGTGCAATTTGGCAAATCCTTTGCATCTTCGTTACCTAAAACTTGAGTTGGATGGGATTGTGCCACAAGTTTTGAGTACGTTTTTTCATCTTCAAGTATTAGATGTTGGATCAAGCATGGATACTTCTCTACCCAATGGCTTGTTGCATAATCTTGTTAGCCTGCGACATCTTGTTGCACACAAGAGAGTCCATTCTTCCATTACTAGCATTGGTAACATGACATCTATCCAGGAGCTACATGATTTTGAAGTTCGAATTTCTAGCGGCTTTGAGATAACACGACTCCAATCCATGAACGAGCTTGTTCAACTTGGGTTGTCTCAACTTGACAGTGTTAAAACCAGGGAGGACGCTTATGGGGCAGGACTAAGAAACAAGGAACACTTAGAAGAGCTTCATTTGTCCTGGAAGGATGCATATTCAGAGTATGAGTATGCCAGTGACACTGAATTTGAATCTTCTGCAAACATGGCAAGAGAAGTGATTGAGGGTCTTGAACCACACATGGATTTAAAACATCTACAAATATCTCAGTATAATGGGACCACTTCACCAGCTTGGCTTGCCAACAATATCTCAGTTACCTCATTGCAGACGCTTCATCTTGATGATTGTGGAGGATGGAGAATACTTCCATCTCTGGGAAGTCTTCCATTCCTTACAAAGGTGAAGTTGAGCAGCATGCTGGAAGTAATTGAAGTACTGATTCCTTCACTGGAGGAGCTAGTTCTAATTAAAATGCCGAAGTTAGTGAGATGCTCAAGCACTTCTGCCGAGGGTCTGAGCTCTAGCTTAAGGGTACTGCACATTGAGGATTGTGAAGCATTGAAGGAGTTTGATCTGTTTGAGAACGATTATAATTCTGAAATCATTCAGGGATCATGGCTGCCTGGTCTTAGGAATTTGATTCTATATTGTTGCCCTCATTTGAAAGTGTTGAAGCCTCTTCCACCTTCAACTACCTTTTCTAAGGTACTCATCAGAGAAATTTCAAGATTTCCGTCTATGGAGGTATCATCTGGTGAGAAGTTACAAATTGGGAATATTGATGTGTACATAGGCGATGATTTTGATGAGTCTTCTGATGAGTTGAGCATACTGGATGACAAAACTTTGGCGTTCCATAATCTTAGAAACCTGAAATCGATGGAGATATATGGTTGCAGAAATCTAAGGTCTTTTTCGTTCGAAGGTTTCAGTCATCTTGTCTCTTTAACAAGTTTGAAAATAGTAAGCTGTGAACAACTTTTCCCTTCAGATGTGACGGCAGAGTATACCCTTGAAGATGTGACAGCTGTGAACTGCAATGCCTTCCCATATCTTAAAAGCCTCAGTATCGACTCATGTGGAATAGCGGGGAAGTGGCTATCGCTGATGCTGCAGCATGCGCCAGGCCTAGAGGAATTGAGTTTAACAAGTTGCGCCCATATAACAAGAGTAGTGTTACCGATGGAAGAGGAAGAAAACAATCTATTAACAACAGTACTGTCATCAGGAAATCAAGATGAGGCATTGACATGGTTAGTTCGTGACGGACTCTTGCACATTCCATCAAATCTCGTCTCCTCTCTCAAGAATATGAGTATTACTCAGTGCCCTCGCCTAAAGTTTAACTCAGGCAAGGACTGCTTCTCTGGATTTACCTCGCTTGAGAAGCTTGAAATTTGGGGATCGTTGGTGGATGATGACGGAAGTGATGACCTGGAGAATGGAAGTTCTTTTGTGTTCGGAGAGGAGGATCAACCCCTGGGGGCGAACGGAAGATGGCTCCTCCCGACATCACTTCAGGAACTTCACATCGTGTCATTGTATTGCCAAGAAACGCTGCAAGTCTGCTTCCCTAGAGATATCACCAGCCTTAAAAAGTTAAGTGTACGTTCCGGCCAAGGTTTGCAATCTCTACAGCTGTACTCATGCACGGCACTGGAAGAATTGGCAATTTCCGGCTCTGGATCGGTCACCGTCACTGTACTAGAGGGCACGCAACCCGCTGGCAGCCTCGGGCGTTTGAATGTATCAGACTGTCCTGGCTTGCCATCACGTTTGGACAGCTTTCCAAGGTTGTGCCCTCGGCTGGAAAGGCTTGACATCAATGACCCATCTGTCCTTACCACGCCATTCTGCAAGCACCTCACCTCCCTGCAACGCCTAAAACTTGGCTTCTTGAAAGTGACGAGACTAACAGATGAGCAAGAACGAGCGCTTGTGCTCCTCAAGTCACTGAAAGAGCTCGAGATTTTTTATTGTACTCATCTCATAGATCTTCCTGCGGGGCTGCAGACCCTTCCTTCCCTCAAGAGTTTGAAGATAGAAGAGGGTCGAGGCATCTCAAGGCTGCCGGAAGCAGGCCTCCCACATTCGCTGGAAGAACTGGAAATCAAAATTTGCAGCAAGCTAGAAGATGAATGCAGGCGGCTAGCAACATGCGAAGGCAAGCTAAAAGTCAAAATTGATGGTCGATATGTGAATTAA >curated_TraesCS2B01G48800_Ta_2B09ATGATGGAGCCGGCGGGAGACTCTTTTGTGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAACGCTCCTGATGGACAAGATGGAGGAGTGGATTCGGCAAGTCGGTCTTGCCGACGACGTCGAGAGGCTCCAGCGCGAGGTCGAGAGAGTCGACATGGTGGTGGCTGCTGTGAAGGGGAGGGCAGCCGGGAACAGGCCTCTGTCCCGGGCTCTCGCTCGTGTCAAGGAGCTTCTCTACGACGCCGACGACGTGGTCGACGAACTGGACTACTACAGGCTCCAACAGCAAGTCGAAGGAGGTAGTAAGCATAATCCCATTATATCGAAACTATTATGATACTTAATACTCCCTCTGTTTCTAAATATAAGTATTTTTAGAAATTTCCGTATGTAGTCCATATTGAAATCTCTAAAAGGAATTATATTTAGTAACGGAGGGCGTAGTTTGATCTTAATTTCTGGTCCATATTTCTTTTCGGCACAGTTACGAGTGACAAGCCTGACGATATGCGTGGAGCTGAAAGAGTGGATGAAATATCAAGGGGCCATGTCGATACACTGAATGTCAGTGTTGGGAAATTACGGTCCTCGGTATGGGAACACTTTACCATCACAGAAACTGTCGACCGGAAGCGTTCAAAAGCCAAATGTAAGTACTGTAGAAAGGATTTTAATTGCGAAACGAAGACAAACGGGACTTCATCTATGAAAAAACATTTGGAGAAAGAGCATTCCGTAACTTGTACGAAGAAACGTGGAGCCCATCCACCAAACCCTTCAAGGTACCCAAAGGAAATTGTATGTTGCACCAGTGCATTTGTATTACAAGTTTATATATATCTGCTTGAGAGCCCATTGTTGCTCTACATTTCTTCTGATAACTGACCCACCATCCGTTTCTTGTTGCAGCACCGGTGATGCGACTTGTAATGTGAGGTCGGTTGAAGTTGGTAGTTCGTCCAACGGAAAAAGAAAGAGAACAAATGAGGATCCAACACAAACCACCGCAGCTAACACACACACCCAATGGGACAAGGCTGAGTTTTCCAATAGGATAATTAAAATTACAGGCCAGTTACAGTCACAGGACATCCAAGGGGCTTTGAGTAAAGTTCTTGGGCCATATGGACCTAGCGCTACTTCAAGTTCAAGTCATCACCGCCCGAGTACAACCTCAGCTCAGCACCCAACAACATCAAGTCTTGTTCCACTGGAAGTTTATGGCAGAGTTGCAGAAAAGAACAAGATCAAAAAGTCAATAACTGAAAACCAATCTGGTGGTGTAAATGTTCTACCTATTGTAGGCATTGCAGGTGTTGGAAAGACAACTCTCGCTCAATTTGTGTATAATGATCCAGACGTGAAAAGTCAATTTCACCACAGGATATGGGTTTGTGTGTCCCGTAAATTTGATGAAGTGAAGCTCACAAAGGAGATGTTAGACTTTTTTCCTCGAGAAAGGTATGAAGGAATTAGCAATTTTGCGAAGCTTCAAGAGATCTTGAAAGAACATATCGAGTACCAGTCGAAGAGCTTTCTGCTTGTATTAGACGATGTCTCGGACAATGTTGATTATCATAAATGGAACAAATTGTTGTACCCTTTGATGTCAAGTCAAGCAAAGGGTAATATAATTCTAGTCACAACCAAAAATTTGTCTGTTGCACAAAGGTTAAGAACACTTGAACCGATCAAGTTAGGTGCTTTAGAAAATGATGATATGTGGTTATTGCTCAAGTCATGTGCATTTGGTTTTGGGGACTACAAAGGTCCGGGAAATCTAAGAGCTATTGGAATGCAAATAGCAGAGAAGTTAAAGGGCAACCCGTTAGCAGCAGTAACTGCAGGGGCACTGTTAAGAGATCATCTTAGCGTTGATCATTGGAGTAACATTCTCAAGAAAGAGAAGTGGAAATCGTTGGGACTCCATGGGGGCATCATGCCTGCTTTGAAGCTTAGCTATGATGAGCTACCGTACCATTTACAACAATGTTTCTCGTATTGTTCTATATTTTCTGAAAAATATAGGTTTCTTCGGAAGGAACTGGTCTATATTTGGATTTCTCAAGGATTTTTGAATCACACTAAGAGATTGGAGGAGATAGGATGGGAATGTCTGAATAATTTGGTGAACCTGGGATTCTTTCAGCAGATTGGAGAGCAACAGGAAGGGGATGAAGATGAGGAAGAAGATTTTTTTCTAGGCAGTAAAATTTGGTATTGTATGTCTGGTCTCATGCACGATTTTGCAAGGATGGTTTCAAGGACTGAGTGTGCAACCATGGATGGTCTTCAGTGTAATAATATGTTACCAACTATACGTCACTTGTCAATTGTGACCAATTCTGCATATAGCAAAGAACAGCATGGAACCATACCTCGCAATATCAAGTTTGAAGAGAACCTGAGAAATGCATTTGCATCAGTGAGGAAATTGAGGACATTAGTTTTATTTGGGCACTACGACTCTTTCTTCTTCAAATTGTTCCTTGATATATTCCAGAAGGACCAGAACTTGCGTCTGCTGCAAATGTCTGCAACATGTGCTGATTTTGATTCCTTCATGTGTAGTTTGGTAAATCCTGCACATCTTCGCTATCTAAAACGTGAACCTGATGAGGTGAATGGTGCTTCCCCTCAAATTTTGAGCAAGTTGTACCATCTTCAAATATTAGATGTTGGCTCATACACTGATCCTATACCTGATGGTAATAATAATCTAGTTAGCCTGCGGCATCTTATTCCAGAAAATGGAGTATACTCTTCCATTGCTAGCATTGGTAGAATGACATCACTTAAAGAGCTACATCATTTTAAGGTTCGGTTTTGTTCTAGAGGATTTGAGATATCACAACTCCAATGCATGAACGAGCTTGTACAACTTGGGGTGTCTCGAGTTGATAGTGTTAAAACTCGGGAGGAGGCTTATGGAGCAGGACTGAGAAGCAAAGAATACTTGAAAAATCTGCACTTGTCCTGGAAGGATACCTTGTCACAGAAGGAATGTGACACTAGCTCTGAATATTCTGCAGACGAAAACGAGGAGCTCTCACAAATGGATACAGCAAGAGAGGTGCTCGAGGGACTTGAACCTCACATGAACTTAAAGCATCTACATATATCTGGGTATAATGGTACTACTTCACCAACTTGGCTTGCCAACAATCTCTCAGTTACCTCCTTGCAGACGCTTCACCTTGATGGTTGTCGAAGATGGAGAATACTTCCATCTCTTGAAAGTCTTCCATTTCTTACAAAGCTGAAGTTGAGCAGCATGCTGGAAGTAATAGAAGTATTGGTTCCTTCACTGGAGGAGCTAGTTTTGATGGACATGCCTAAGTTAGTGAGATGCTCAAGCATTTCTGTGGGGGCTCTGAACTCTAGCTTACGAGCACTACGGATCGAGGATTGTGAAGCACTAAAGGAGTTTGATCTGTTTGAGAACGATGATAATTCTGAAATCATTCAGGGGTCATGGCTGCCTGGTCTTAGGAATTTGATTGTGAAATGTTGCCCTCATTTGAAAGTGTTGAAGCCTCTTTCACCTTCAACTACCTTTTCTAAGGTAGTCATCAGAGAAGTTCCAAGATTTCCGTATATGGAGGTATCATCTGGTGAAAAGTTAGAAATTGGGAAATTTGATGAGGACGGAGATGATTTTGATGAATCTTGTGATGAGTTGAGGATACTGGATGACAAAATTTTGGCATTCCACAATCTTAGAAACCTCAAATCGATGGAGATATATGGTTGCAGAAATCTAAGGTCTTTTCTGTTCGAAGGTTTCAGTCATCTTGTCTCTTTATTAAGTTTGGATATAACAAAGTGTGAACAACTTTTCTCTTCGGATATGTCGCCAGAGTATACCCTTGAAGATGTGAGAGCTGTGAACTTCAATGCCTTCCCATTTCTCAAAAATCTCAGTATTGACTCATGCGGAATAGCGGGGAAGTGGCTATCGCTGATGCTGCAGCATGCGCCAGGCCTAGAGGAATTGCGTTTAAGATATTGCGCACATATAACAAGAGTAGTGTTACCGATGGAAGAGGAAGAAAACAGTCTCTTAACAACAGTAGTGTCATCAGGAAATCAAGATGAGGCATTGACCTGGTTAGTTCGTGACGGACTCTTGCACATTCCATCAAATCTCGTCTCCTCTCTCAAGAAGATGACTATTGGTCAGTGCCCTCGCCTAAAGTTTAACTCGGGCAAGGACTGCTTCTCTGGATTTACCTCGCTTGAGAAGCTTGAAATTTGGGGATCATTGGTGGATGATGACGGAAGTGATGACCTGGAGAATGGAAGTCCTTTTGTGTTCGGAGAGGAGGATCAACCCCTGGGAGCGAATGGAAGATGGCTCCTCCCGACATCACTTCAGGAGCTTAACATCGGGTGGTTCTGTTACCAAGAAACGCTGCAACCCTGCTTTCCTAGAGATATCACCAGCCTTAAAGAGTTAAGTGTACGTTCAATCCAAGGTTTGCAATCTCTACAGCTGCACTCATGCACGGCACTGGAAGGATTGGAGATTAGAGGCTGTGAATCGCTCACCGTCACTGTACTAGAGGGCATGCAACCCATTGGCAGCCTCGTGCGTTTGAATGTATCAGACAGTACTGGCTTGCCACCATGTTTGGAGAGCTTTTCAACGCTGTGCCCTCGGCTTGAAAGGCTTTGCACCGATGACCCATCTGTCCTTACCACGTCATTCTGCAAGCACCTCACCTCCCTACAAAGACTAGAACTTAGTTTCTTGAAAGTGACGAGACTAACAGATGAGCAAGAGCAAGCGCTTGTGCTGCTCAAATCCCTGCAAAAGCTCGAATTCATTTGGTGTTCTGCTCTAGTAGTTCTTCCTGAGGGGCTGCACACCCTTCCTTCCCTCAAGAGATTGGAGATAAACCAGTGTGGACGCATCACAAGGCTGCCAGAAGCAGGCCTCCCACATTCGCTGGAAGAACTCGAAATCCGGTCTTGCAGCCAGGAGCTAGATGATGAATGCAGGCGGCTAGCAACAAGCAAACTGAAAGTCAAGATTGATTGGACGTATGTGAATTAA >curated_TraesCS2901G48800_Ta_2B09MMEPAGDSFVEAAIAWLVQTILATLLMDKMEEWIRQVGLADDVERLQREVERVDMVVAAVKGRAAGNRPLSRALARVKELLYDADDVVDELDYYRLQQQVEGVTSDKPDDMRGAERVDEISRGHVDTLNVSVGKLRSSVWEHFTITETVDRKRSKAKCKYCRKDFNCETKTNGTSSMKKHLEKEHSVTCTKKRGAHPPNPSSTGDATCNVRSVEVGSSSNGKRKRTNEDPTQTTAANTHTQWDKAEFSNRIIKITGQLQSQDIQGALSKVLGPYGPSATSSSSHHRPSTTSAQHPTTSSLVPLEVYGRVAEKNKIKKSITENQSGGVNVLPIVGIAGVGKTTLAQFVYNDPDVKSQFHHRIWVCVSRKFDEVKLTKEMLDFFPRERYEGISNFAKLQEILKEHIEYQSKSFLLVLDDVSDNVDYHKWNKLLYPLMSSQAKGNIILVTTKNLSVAQRLRTLEPrKLGALENDDMWLLLKSCAFGFGDYKGPGNLRAIGMQIAEKLKGNPLAAVTAGALLRDHLSVDHWSNILKKEKWKSLGLHGGIMPALKLSYDELPYHLQQCFSYCSIFSEKYRFLRKELVYIWISQGFLNHTKRLEEIGWECLNNLVNLGFFQQIGEQQEGDEDEEEDFFLGSKIWYCMSGLMHDFARMVSRTECATMDGLQCNNMLPTIRHLSIVTNSAYSKEQHGTIPRNIKFEENLRNAFASVRKLRTLVLFGHYDSFFFKLFLDIFQKDQNLRLLQMSATCADFDSFMCSLVNPAHLRYLKREPDEVNGASPQILSKLYHLQILDVGSYTDPIPDGNNNLVSLRHLIPENGVYSSIASIGRMTSLKELHHFKVRFCSRGFEISQLQCMNELVQLGVSRVDSVKTREEAYGAGLRSKEYLKNLHLSWKDTLSQKECDTSSEYSADENEELSQMDTAREVLEGLEPHMNLKHLHISGYNGTTSPTWLANNLSVTSLQTLHLDGCRRWRILPSLESLPFLTKLKLSSMLEVIEVLVPSLEELVLMDMPKLVRCSSISVGALNSSLRALRIEDCEALKEFDLFENDDNSEIIQGSWLPGLRNLIVKCCPHLKVLKPLSPSTTFSKVVIREVPRFPYMEVSSGEKLEIGKFDEDGDDFDESCDELRILDDKILAFHNLRNLKSMEIYGCRNLRSFLFEGFSHLVSLLSLDITKCEQLFSSDMSPEYTLEDVRAVNFNAFPFLKNLSIDSCGIAGKWLSLMLQHAPGLEELRLRYCAHITRVVLPMEEEENSLLTTVVSSGNQDEALTWLVRDGLLHIPSNLVSSLKKMTIGQCPRLKFNSGKDCFSGFTSLEKLEIWGSLVDDDGSDDLENGSPFVFGEEDQPLGANGRWLLPTSLQELNIGWFCYQETLQPCFPRDITSLKELSVRSIQGLQSLQLHSCTALEGLEIRGCESLTVTVLEGMQPIGSLVRLNVSDSTGLPPCLESFSTLCPRLERLCTDDPSVLTTSFCKHLTSLQRLELSFLKVTRLTDEQEQALVLLKSLQKLEFIWCSALVVLPEGLHTLPSLKRLEINQCGRITRLPEAGLPHSLEELEIRSCSQELDDECRRLATSKLKVKIDWTYVN- >curated_TraesCS2B01G488400_Ta_2B10ATGGCGGCCGCGATTgGGTGGCTGGTTGAGACCATCTCTGCGACCCTCCAAATCGACAAGCTCGACGCCTGGATTCGGCAAGTCGGTCTTGCCGATGACATCGAGAAGCTCAAGTCGGAGATCCGGAGAGTCAACATAGTGGTCACTGCTGCCAAGGGCAGGGGGGTAGGGAGCGAGCTGCTGGATGGACCTTTCGCTCTTCTGGAGGAGCGGCTCTATGAAGCCGACGACGTGGTCGACGAGCTCGACTACTACAGGCTCCAACACCAAGTCCAAGGTCTGCCGGCACCTGCAGATCCAAGCGAGCCAGTCCCACTCCCAGTCCCAGGAGGTAAGCGTAAATCTGTCTAGACCCAAGTAATCCAAGTCTGCTAATTATTAGTTTGATCTTATGTTGCTCCAAAAATGTAAATTGGTCGTATCTGATCAAGGACGACCGTTCTTTAATTTCTGGTCCACGATTTCTTTTGGCACAGTTACAAGGGGTGAGCCCGAAGGCGTGCTTGTAGCTGAGCAATTCAATGAGATATCGAGGGGCGGTGGTGATGTACCACAGAGCAATGTTGGCAAATTACGGTCCGTGGTATGGGAACACTTTATGATCACAGAAAGAGATAACGGAAAACCCAACAAGGCAGTATGCCGACACTGTAGCAATGAGTTTAAGTGTGACACCAAGACGAACGGTACATCATCTATGAAAAAGCATTTGGAGAATGAGCATTCTGTGACTTGTACAAAGAAACCTCCTGGAGCACATCTACCAAACCCTTCAAGGTACTTAAAAGAGAATTGGGTATAGAGAGTAGAGTATTCTTTCTAATCTTAAGTGTACATTTTTAAAAAGTTGTTTATATACATATGCTTGAGGCGATTGTGGTCCTGATTAATAAGCACATCCCCCGCAAAATAAATAAATACGCACCTCTTTTTTTCTCACCACAGCACCGGTGAGCCTACTATAATTGCCAGCTCATCCAGCAAAAAACGAAAGAGACGACGGTCCAAGGCATGGGAATTTTTTGATGTCATAGAAGAAGTAAACGAACAGCCTATGAAAGCAAGATGTAAATACTGTCCCGCAGAGATCAAGTGCGGCCCAACAAGTGGGACAGCAGGTATGCTCAACCATAACAAGATTTGTAAGAACAAACCTGGACCAAATGACCAGTTGCCAAACCTGTCAAGGTAACTAAAGAATCTATATGTTGCGTCGAAAAACAATTAGAAGTCATTAAGTTAAGAGTCTCATTGTGGTTCTAATAGTCAATTAACGTTCTTTTTTCTTATTGTAGCACCGGTGATGCTAATGCGGATGTGACGCCAATTCTAATAGGTAACTCGTCCACCAGAAAAGGGAGAATGGATGATTCCATACAAATTGATGTGACTAACACAGTCACCCCTTGGGACATGGCCGAATTATCCAGCAGGATACGAAAAATAGCTAGTCAGTTGCAATACATCCAAGAGGAAACGACTGAAATTCTCAAGCTACATGGATCGGACTCTACTTCAAGTTCAGATCATCACCAGAGTACAACATCATATCAGCACCTCAGAACATCAAGTCTTGTTCCAAGGAATGTGTATGGAAGAGTTAAAGAAAAGGAACACATCATGAAATTGATGATGACAGAAGGCAGATCTGACAAAGTAATTGTTGTGCCTATTGTAGGCATTGCAGGTATTGGAAAGACAACTCTCACTCAACTTGTGTACAACGATCCAGAAGTGGAAAGGCAATTTGAACATAGGATATGGGTTTGGGTGTCTCGCAACTTTGATGAAATGAGGCTCACAAGGGATATGCTGAGCTTTGTTTCTCAAGAAAGTCATGAAGGAATAGGCTGCTTTGGGAAGCTTCAGGAGATCCTGAGAAGTCATGTCAAATCAAAGAGGGTTTTACTTATTTTAGATGATGTATGGTATGACAAGAAAGATGCCCGATGGAACCAACTATTGGCTCCCTTTAAGCCTCATAGTGCCAATGGCAATGTGATTCTTGTGACAACTAGAAAAATGACCGTTGCAAAAATGATTGGAACAGTGGTGCCAATTAAGTTAGCTACTATTGAAAATGATGACTTTTGGTTATTATTCAAATCATGTGCTTTTGTTGATGGAAACTATGAATGTCTTGGAAATCTTAGCACTATTGGACGGCAAATAGCAGAAAAGTTAAAGGGTAACCCGTTAGCAGCAGTGACTACAGGGGCACTATTAAGGAACCAACTTACCGTTGATCATTGGAGTAAAATTCTCAAGGAAGAAAATTGGAAATCATTAGGACTTAGTGGAGGCATCATGCCTGCTTTGAAGCTTAGTTATGATGAGTTGACATACCGTTTACAACAATGTTTCTTGTATTGTTCTATATTTCCTGACAAATATAGGTTTCTTGGTAAGGATTTGGTATATATGTGGATTTCTCAGGGATTTGTGAATTGCACCCAAAATAAGAGATTGGAGGAGATAGGATTGGAATATCTGAATCATTTGGTAAACCTGGGATTCTTTCAGCAAATTGAAGAACAGCAAGAATTGGATGAGGAAAAAGAATTCTCTCTACGCGGTCAGATTTGGTATTCTATGTGTGATCTCATGCATGATTTTGCGAGGATGGTTTCGGTGACTGAATATGCGAGGATAGATGGTCTGCAGTGTAAGAAAATCTTACCGACTATACACTATTTGTCAATAGTAACTGGTTCTGCATACAACAGAGATCTGCATGGGAATATTCCTCGCAATGAGAAGTTTGAAGAAAATCTGAGAAATTCTGTTACATCAGTTACCAAATTGAGAACACTGGTTGTACTTGGGAGCTTTGACTATTTCTTTGTACAGTTGTTCCAAGATATATTTCAAAAGGCCCAAAATTTACGCCTGCTGCGAGTATCTCCAGAATCCACTTATCTGTTTCAAGTGCCTGCAGCATCCACTGATTTTAATTCCTTCCTGTGCAGTTTGGCAAATCCTTTGCATCTTCGTTATCTAAAACTTGATTTAGACGGGATTGTGCCACAAGTTCTCAGTACTTTTCTTCTTCTTCAAGTATTAGATGTTGGCTCAAACAGGGATACTTCTCTACCCAATAGCTTGCATAATCTTGTTAGCCTGCGACATCTTGTTGCACACAAGAGAGTCCATTCTTCCATTGCTAGCATTGGCAACATGACATCTATCCAGGAGCTACATGATTTTGAGGTTCGAATTTCTAGCGGCTTTGAGATTACACAACTCAAATCCATGAACAAGCTTGTTCAACTTGGAGTGTCTCAACTTGACAGTGTTAAAACCCGGGAGGAGGCTTATGGGGCAGGACTAAGAAACAAGGAACACTTAGAAGAGCTTCACTTGTGTTGGAAGCATGCATTTTCAGTGGATAAGGATGTCAGTGACACTAGATTTGAATCTTCTGCAGACATGGCCAGAGAAGTGATTGAGGGTCTTGAACCACACATGGATCTAAAACATCTACAAATATCTCGGTATAATGGTACCACTTCACCGACTTGGCTTGCCAATAATATCTCAGTTACCTCACTGCAGACGCTTCATCTTGATGATTGTGGAGGATGGAGAATACTTCCATCTCTGGGAAGTCTTCCATTTCTTACAAAGTTGAAGTTGAGCAACATGTGGGAAGTAACAGAAGTATTGGTTCCTTCACTGGAGGAGCTAATTTTACTCAACATGCCCAAGTTAGTGAGATGCTCAAGTACTTCTGTGGGGGCTCTGAACTTTAGTTTACGAGCACTGCGGATCGAGGATTGTGAAGCACTGAAGGAGTTAGATCTGTTTGAGAACGATGATAATTCTGAAATCATTCAGGGGTCATGGCTGCCTGGTCTTAGGAATTTGATTGTGAAATATTGCCCTCATTTGAAAGTGTTGAAGCCACTTCCACCTTCAGCTACCTTTTCTAAGGTACTCATCAAAGTGGTTTCAAGATTTCCGTCTATGAAGGTATCATCGGGTGAAAAGTTAGAAATTTGGGATGCTAATTACCGCAGAGGCGATCGATCTTGTGATGAGTTGATCATACTGGATGACAAAATTTTGGTGTTCCATAATCTTAGAAACCTCAAATCGATGGAGATATTTGGTTGCAGAAATCTAAGGTCTTTCTCGTTTGAAGGTTTCAGTCATCTCGTCTCTTTAACGAGCTTGAAAATAAGAGGCTGTGAAAAACTTTTCTCTTCACATGAGATGCCAGCCATTGAACATGTGACAGCTGTGAACTGCGATTCTTTCCCATCTCTTAAAAGTCTCAGTATTAAGTCATGTGGAATAGCGGGGAAGTGGCTATCGTTGATGCTGCAGCATGCGCCAGGCCTAGAGAAATTGAGTTTAAGATATTGCGCACATATAACAACAGTACTGTTACCGATGGAAGAGGAAGAAAACAATCTATTAACAACAGTACTGTCATCAGGAAATCAAGATGAGGCATTGACCTGGTTAGCTCGAGAGGGACTCTTGCACATTCCATCAAATCTCGTCTCCTCTCTCAAGAATATGAGTATTAGTGAGTGCCCTCGTCTAAAATTTAACTGGGGCACGGACTGCTTCTCTGGATTTATCTCGCTTGAGAAGCTTGAAATCTGGGGATCGTTGGTGGATGATGACGGAAGTTATGACCCCGAGAATGGAAGTTCTTTTGTGTTCGAAGAGGAGGATCAACCCCTGGGGGCGAACGGAAGATGGCTCCTCCCGACATCACTTCAGGAACTTAACATCAGGTTCTTGTGTTACCAAGAAACGCTGCAACCCTGCTTTACTAGAGATATCACCAGCCTTAAAAAGTTATATGTAAGCTTCAGCCCAGGTTTGCAATCTCTACAGCTGCACTCATGCACGGCACTGGAAGAATTGGCAATTGTCGGCTGTGGATCAGTCACCGTCACTGTACTAGAAGACTCTCCTGGCTTGCTGCCATGTTTGGAAAGGCTTTGCATCAATGACCCATCTGTCCTTACCACGTCATTCTGCAAGCACCTCACCTCCCTGCAACGCCTACGACTTGGTTTCTTGAAAGTGAGGAGACTAACAGATGAGCAAGAGCAAGCGCTTGTGCTGCTCAAATCCCTGAAAGAGTTCCAATTCTATTTGTGTAATGATCTCGTAAATCTTCCTGCTGGGCTGCACACCCTTCCTTCCCTCAAGAGGTTGGAGATAGAACGGTGTGGACGCATCTCAAGGCTGCCAGAAGCAGGCCTCCCACATTCGCTGGAAGAACTGAAAATCGAGTCTTGCAGCCAGGAGCTATATGATGAATGCAGGCAGCTAGCAACAAGCAAACTGAAAGTCAAAATTGGTGGGAGATATGAGAATTAA >curated_TraesCS2B01G488400_Ta_2B10MAAAIGWLVETISATLQIDKLDAWIRQVGLADDIEKLKSEIRRVNIVVTAAKGRGVGSELLDGPFALLEERLYEADDVVDELDYYRLQHQVQGLPAPADPSEPVPLPVPGVTRGEPEGVLVAEQFNEISRGGGDVPQSNVGKLRSVVWEHFMITERDNGKPNKAVCRHCSNEFKCDTKTNGTSSMKKHLENEHSVTCTKKPPGAHLPNPSSTGEPTIIASSSSKKRKRRRSKAWEFFDVIEEVNEQPMKARCKYCPAEIKCGPTSGTAGMLNHNKICKNKPGPNDQLPNLSSTGDANADVTPILIGNSSTRKGRMDDSIQIDVTNTVTPWDMAELSSRIRKIASQLQYIQEETTEILKLHGSDSTSSSDHHQSTTSYQHLRTSSLVPRNVYGRVKEKEHIMKLMMTEGRSDKVIVVPIVGIAGIGKTTLTQLVYNDPEVERQFEHRIWVWVSRNFDEMRLTRDMLSFVSQESHEGIGCFGKLQEILRSHVKSKRVLLILDDVWYDKKDARWNQLLAPFKPHSANGNVILVTTRKMTVAKMIGTVVPIKLATIENDDFWLLFKSCAFVDGNYECLGNLSTIGRQIAEKLKGNPLAAVTTGALLRNQLTVDHWSKILKEENWKSLGLSGGIMPALKLSYDELTYRLQQCFLYCSIFPDKYRFLGKDLVYMWISQGFVNCTQNKRLEEIGLEYLNHLVNLGFFQQIEEQQELDEEKEFSLRGQIWYSMCDLMHDFARMVSVTEYARIDGLQCKKILPTIHYLSIVTGSAYNRDLHGNIPRNEKFEENLRNSVTSVTKLRTLVVLGSFDYFFVQLFQDIFQKAQNLRLLRVSPESTYLFQVPAASTDFNSFLCSLANPLHLRYLKLDLDGIVPQVLSTFLLLQVLDVGSNRDTSLPNSLHNLVSLRHLVAHKRVHSSIASIGNMTSIQELHDFEVRISSGFEITQLKSMNKLVQLGVSQLDSVKTREEAYGAGLRNKEHLEELHLCWKHAFSVDKDVSDTRFESSADMAREVIEGLEPHMDLKHLQISRYNGTTSPTWLANNISVTSLQTLHLDDCGGWRILPSLGSLPFLTKLKLSNMWEVTEVLVPSLEELILLNMPKLVRCSSTSVGALNFSLRALRIEDCEALKELDLFENDDNSEIIQGSWLPGLRNLIVKYCPHLKVLKPLPPSATFSKVLIKVVSRFPSMKVSSGEKLEIWDANYRRGDRSCDELIILDDKILVFHNLRNLKSMEIFGCRNLRSFSFEGFSHLVSLTSLKIRGCEKLFSSHEMPAIEHVTAVNCDSFPSLKSLSIKSCGIAGKWLSLMLQHAPGLEKLSLRYCAHITTVLLPMEEEENNLLTTVLSSGNQDEALTWLAREGLLHIPSNLVSSLKNMSISECPRLKFNWGTDCFSGFISLEKLEIWGSLVDDDGSYDPENGSSFVFEEEDQPLGANGRWLLPTSLQELNIRFLCYQETLQPCFTRDITSLKKLYVSFSPGLQSLQLHSCTALEELAIVGCGSVTVTVLEDSPGLLPCLERLCINDPSVLTTSFCKHLTSLQRLRLGFLKVRRLTDEQEQALVLLKSLKEFQFYLCNDLVNLPAGLHTLPSLKRLEIERCGRISRLPEAGLPHSLEELKIESCSQELYDECRQLATSKLKVKIGGRYEN- >curated_TraesCS2B01G488600_TraesCS2B01G488700_Ta_2B11ATGGAGGCCGCGATTGCATGGCTGGTGCAGACCATCCTTGCAACCCTCCTGATCGATAAGCTCGATGCGTGGATTCGGCAAGTCGGGCTTGCCGATGACGTTGAAAAGCTCAAGTCAGAGATCAGGAGAGTCAAGATGGTGGTCTCGGCTGTGAAGGAGAGAGGGATCAGGAACGAGTCGCTGGATGAATCTCTCGCTCTTCTCGTGGAGCGACTCTACGAAGCCGACGACGTGGTCGACGAGCTGGATTACTACAGGCTCCAAGAGCTGGTTGAAGGTGCCCGGCCCCGGCTGCCTGCAGATCCAACCGTGCTGGTTCCTTCCAACCTGCCCATCCAAGGAGAAGGAGGTACGCATACTTCTTCCTGTAGATCCAACACAAAGTTCTTTCATAGGCCGAGTATCGAAGTGTGACAAACTACTAGTAATTGTTAGTCTGATGATCCTATCTTACTTAGGACAAATTAATGAAATTTATATTATCTGATCAAGGACGACCATGCTTTTCTGGTCCATTTTTCTGTTGGCACAGCTACAAGAAACGAGCCCGAAGGTAACAGTGCTGGCAAATCACGGTCCGTGGTCTGGGAAAACTTTACAGTCACAGAAACTGTTGACAGAAAGTCCGCCAAAGCAGTATGTAGACACTGTGGCAATGAGTTCAAGTGTGATACGAAGATCAACGGTACATCATCTATGAAGAAACATTTAGAGAAGGAGCATCCCGATAAGATGAAACCTCCTGGAGCGCATCCACCAAACCCTTCAAGGTACCTAAAGAAGAATTGAGCATGAGCCCATTTAATTAGAAATCGTTTATATACCTCTTTCTTTTTTCTTGAATGGTTATATACATCTTCTTGACAGCGCACTAATTTTGGTCCTAATAGCCAACCCACCACTTTTTTCTTACTGCAGCACTGCTGAGCCTATTGCCATTGCCAGCTCATCCAGGGGAAAAGGAAAGAAACAGCGGTCCAAGGCATGGGATAATTTTGATGTTATAGAAAATGACATTGGACAGCCAACCAAAGCAATATGTAAATACTGCCACACAGAGATCAAGTGCGGAATGAAGACCGGGACAGCGGGTATGCTTAACCATAACAAGATTTGCAAGAAGAAACCTGAACCAAATGACCAGCCACCAAACCTGTCGAGGTAGCTACCTTGCATCAGCAAATTTTTGGATGTTGTTTTATAAACAATCCCCACCATGGTTCTAATAGCCGTTTGTTCATGATCTTTTTCTTACTGCAACATTGGTGATGCTACTGCAAATGCGACATATATTGTGGTTTATGACGATTCAGCTACAAGAAAAAGAAGGAGAGTGGATGAGGAGTCAGCAGAAATCACTGCAGCTAATACACACACCTGTTGGGACAAGGCTACATTATCCAATATGATACGAAAAATTATTAGTCAGTTACAAGAGATCCAAGGGCAAGTGAGGGAGGTTATCGAGTTACATGGATCAGACTTATCTTCCAGTTCAAATCACCATCAAAATACAACCTTATATCAGCGCCTACGGACATCAAGTCTTGGTCCAAGAAAAGTGTATGGAAGAGTTGCAGAAAAGAACTCCATTGTAAGGATGATAACAGGAGAAAAGTCTGGTGGTTTAGTTGTTCTGCCTATTGTAGGCATTGCAGGTGTTGGCAAAACAACTCTTGCTCAACTTGTATACAATGATCCATATTTGGATGATCATTTTGACCAAAGGATATGGGTTTGGGTGTCTCGCAATTTTGATGAAGTGAGACTAACAAGGGAGATTTTGAACTCTGTTTATCAAGAAAGGCATGAAGATATAAAATGTTTTGCGAAGCTTCAGGAGATCTTGAAGCATCAGGCCGACTCACAGCGACTTTTAATCATTTTAGATGATGTCTGGGATGACATGAACGATAATATCCAACACCATAAAATGTTGGCTCCTCTGGTATCAAGTCATGTGAAGGGTAATGTGATTCTAGTCACAACCAGAAGTATGTCTGTTGCACAAAGCTTAGGCACCCTCAAGCCAGTCAAGTTAGGTGCTCTGGCAAATGATGACTTTTGGTTATTGTTCAAATCACACGCATTTGGTTACGAGAACTGTCAGGAGCATCAAAGTTTAAGTATCATCGGGCGGCAAATAGCCGAGAAGTTAAAGGGCAACCCATTAGCAGTTGTATCTACAGCAGAACTATTACGGAAGAAACTTAACACCGATTATTGGAGAATCGTTCTAAAGAACGAAGAGTGGAAATACATGCATCACAATAGAGGGATCATGGCTGCTCTGAAGCTTAGCTATGATCAACTTCCGTACCATTTACAACGGTGTTTCTCATATTGCTCCATATTCCCTGACAGTTATCAGTTTCTTAGTGAGGAGTTGGTCGGTTTCTGGATATCACAGGGATTTGTAAAGTGCAACGGCTCTAGTCAGAGATTGGAGGATATAGGGCGGGGATATCTGATTGATTTGGTTAACCTGGGCTTCTTTGAAGAAGCTAAAAGAGAAGAACCATATCTAGGCAGTCAAGTTATGTATGCCATATGCGGTCTCATGCATGATTTTGCGATGATGGTTTCAAGGACTGACAGTGCAAGTATAGATGGTCGACCCTACAAAAAAATGCCTCGAACTCTACGACATTTGTCAATAGTAAATGGATCCGCATACCAGAAAGATCAGCATGGGAACATTTATCATGATGAGAAGTTTGAAGAAAATCTGAAAAATGCAATTACATCAGTTAGTGAACTGAGGACATTAGTGTTACTTGGGCACTATGACTTTTCCTTCTTACTATTATTCCAATATATATTCCAAAAGGCACATAACTTACGTGTGCTACAAATGTCTGCAGCATCTGCTGATTTTCTCAAACATGGGATTGAGGAGGTGGATGGGTCTTTCCCTCAAATTTTGAGCAAATTGTACCATCTCCAAGTATTAGTCGGTTCATACAATGATCGTACTATGCCTGGTTGTATTGATAATCTTGTTAGCCTGCGGCATCTTGTTGTACACAAGGGAGTGTACTCTTCCATTGCAACCATTGATAATATGCTATCATTTCAGGAACGACATGGTTTCAAGTTTCATATTTCTAGTGGCTTTGAGATAACACAACTCCAATCCACTGAACATTGGATGCATGTTAATACTCTGGAAGATGTTTATGAGGCAGGACTGGTAAACAATGAACTCTCAGAAAAGTTGCACTTGTCCTGGAAGGATTCTCCTGCGGACATGGTCATGGAGGTTGAGGGTTGGGAACCACATTGSGACTTAAGGGTTCTCGAGATATCTGGGTATAATTTTGCTTGGACAATTATGGTTGACAACATTATCTTGGTTACCTCCTCCCAGACGGTTCACATATGCGATTGCATTGAATGGAAAATACTTCCATCTTTGGAAAGGTTTCGGTTTTTGACAAAGCTGGAGTTGAGAAACCTGCCTAAAGTAATACAAATACTGGTTCCTTCACTGGAGGAGCTAGCTTTAGTTAAAATGCCAAAGTTGGAGAAATGTACATGCACTTCCGTGGAAGGTATGAGCTCTAGACTAAGAGCACTGCAGATCAAGGATTGTCAATCACTGAAGGAGTTTGATCTGTTTGAGAACAACGATAAATTCGAAACTGGGCAGAGGTCATAGGCTCCTAGTCTTAGGGAACTAAGTCTGGAGAATTGCCCCCATTTGAAAGTGTTGAAGCCTCTTCCACGCTCAAGCATGTGTTCTGAGTTACTCATCTGTGACGTTTCAACACTTCCGTACATGAAGGGATCATCTGATGAAGAGTTATGTATTGGGTATGATGGTGAGTATGGCTATGGTTTTGACGAATCTTCCGATGAGTTGAAGATACTGGATGACAAAATTTTGCTGTTCCATAATCTGAAAAACCTCAAATCGATGGTGATACATGGTTGCCGGAATCTAAGTTCCATTTCATTAAAAGGTTTTAGTTACCTCGTCTCTTTAACGAGCTTGAAAATAAGAAATTGTGAAAAACTTTTTGCTTCAAATGAGATGCCAGAGCATACCCTCGAAGATGTGACACTTGTGAATTGCAAGGCTTTCCCATCTCTGGAATGTCTCAGTATTGATTCATGTGGTATAGTGGGGAAGTGGCTATCTTTGATGCTGCAACATGCGCCATGCCTAGAGGAATTGTATTTGTCTTCCCAAGAGGAAGAAAAATCAGAAGAGGAAGAAAACAGTATATCAAATCTTAGCTCAACCAGGGAGGGCACATCATCCGGAAATCCAGATGACGGATTAGCTCTAGACCGACTGTTGTGCATCCCATTAAATCTCATCTCCATTCTAAAGAGGATAACTATTGAGAGGTGCCCTCATCTAACATTTAACTGGGGCAAGGAAGGCGTCTCGGGATTTACCTCCCTTGAGAAGCTAGTCATTTTAGACCGCCCTGACCTGCTCTCGTCGTTGGTGCATACAGACGGAGGATGGCTACTCCCGAACTCACTTGGCCAACTTGAAATCGATGGCCATTCCCAAGTAA >curated_TraesCS2B01G488600_TraesCS2B01G488700_Ta_2B11MEAAIAWLVQTILATLLIDKLDAWIRQVGLADDVEKLKSEIRRVKMVVSAVKERGIRNESLDESLALLVERLYEADDVVDELDYYRLQELVEGARPRLPADPTVLVPSNLPIQGEGATRNEPEGNSAGKSRSVVWENFTVTETVDRKSAKAVCRHCGNEFKCDTKINGTSSMKKHLEKEHPDKMKPPGAHPPNPSSTAEPIAIASSSRGKGKKQRSKAWDNFDVTENDIGQPTKAICKYCHTEIKCGMKTGTAATRKRRRVDEESAEITAANTHTCWDKATLSNMIRKIISQLQEIQGQVREVIELHGSDLSSSSNHHQNTTLYQRLRTSSLGPRKVYGRVAEKNSIVRMITGEKSGGLVVLPIVGIAGVGKTTLAQLVYNDPYLDDHFDQRIWVWVSRNFDEVRLTREILNSVYQERHEDIKCFAKLQEILKHQADSQRLLIILDDVWDDMNDNIQHHKMLAPLVSSHVKGNVILVTTRSMSVAQSLGTLKPVKLGALANDDFWLLFKSHAFGYENCQEHQSLSIIGRQIAEKLKGNPLAVVSTAELLRKKLNTDYWRIVLKNEEWKYMHHNRGIMAALKLSYDQLPYHLQRCFSYCSIFPDSYQFLSEELVGFWISQGFVKCNGSSQRLEDIGRGYLIDLVNLGFFEEAKREEPYLGSQVMYAICGLMHDFAMMVSRTDSASIDGRPYKKMPRTLRHLSIVNGSAYQKDQHGNIYHDEKFEENLKNAITSVSELRTLVLLGHYDFSFLLLFQYIFQKAHNLRVLQMSAASADFLKHGIEEVDGSFPQILSKLYHLQVLVGSYNDRTMPGCIDNLVSLRHLVVHKGVYSSIATIDNMLSFQERHGFKFHISSGFEITQLQSTEHWMHVNTLEDVYEAGLTEDGYSRTHLANLKSMAIPK - >curated_TraesCS2B01G734100LC_Ta_2912GTATATTGTTTCTGCTCTGCTCGCGTGCTCCCCACCCTCGAGCCTCGACTCCCCCCACACTCTCCACTGACAAGAAACCATCTCCAGCGAACATCTTCTGCCGGATCTGATGGCGGCCTCGATTGGGTGGCTGGTTGAGACCATCTCTGCAACCCTCAAGATCGATAAGCTCGATGCCTGGATTCGGCAAGTCGGACTTGCCGATGACATCCAGAAGATCAAGTCGGAGATCTGGAAAGTCCAGACAGTGGTCACTACTCTACTGCCAAGAGTACGGGGGTCGCAAACGAGCTTCTGGATGAAGCTTTCGCTCTTGTCGAAGAGCGGCTCTATGAAGCCGACGATCTTGTCGACGAGCTCGACTACTACAGGCTCCAACACCAAGTCCAAGGTCTGCCTGCCCCTGCAGATCCAAGCGAGCTACTCCGAAGAGGTAAGCGTAAATCTCTCTACACCCAATTAATCCAAGTCAGCTAATTATTAGTTTGATCTTATATTGCGCCAAAAATTTAAATTGGTCGTATCTGATCAAGGACGCCATTGCTTTTCTGCTCCACGATTTCTTTTGGCACAGTTACAAGGGGTGAGCCCGAAGGTGTGCTTGTAGCTGAGCGACTCAATGAGATACCGAGGGGTGATGGTGATATAGCACAGAGACAGAGCAATGTTGGCAAATTACGGTCCGTGGTATGGGAACACTTCACGATCACACAAAGAGATAATGGAAAACCTGTCAAAGCAGTATGTGTACACTGTAGAAATGAGTTTAAGTGCGATACGAAGACGAACGGTACATCATCTATGAAAAAGCATTTGGAGAATGAGCATTCTGTGACTTGTGCAAAGAAACCTCCTGGAGAACATCCAGCAAACCCTTCAAGGTACTTAAAAGAGAATTGGGTATAGAGTAGAGTATTCTTTCAAGCTCAGATGTACATACACCCCTTACCTTGTACTCCCTCCGTTCCATATTAATCGTCGCTGATTAGTACAACTAATATGGAACGGAGGGAGTATGAGGGAGGCTATGAGCACATTTAAGAAAAAAGTGTTCATATACATCTGCTTGAGGCCATTATATGTTCCTAATAACCCCATCTTTTTATTACTGCAGCACCGGTGAGCCTACTGTAATTGGCAGCTCATCCAGCAGAAAAGGAAAGAGACGACGGTCCAAGGCATGGGAACTTTTTGATGTCATACAAGAAGTAAACGAACAGCCTATGAAAGCAAGATGTAAATACTGTCCCACAGAGATCAAGTGCGGACCAACGAGTGGGACAGCAGGTATGCTCAACCATAGCAAGATTTGTATACCTGGACTAAACAACCAGCCGCCAAACCCGTCAAGGTAACTAAAGAATCTATACATTGCACCGAAAAATATTAGAAGTCATTAAGTTAAGAGTCTCACTGTGGTTCTAATAGCCAATTCACGGTCTTTTTCCTATTGCAGCACTAGTGATGCTAATGCAAATGTGACGCCAATTACTGCGGCTAACACGGTCACCCCTTGGGACATGGCTGAATTGTCCAACAAGATTAAAAAAATAGCTGGTCAGTTGCAATACATCGGAAGGGAAGTGGGTGAGATTCTAAAGCTACATGGATCCGACTGTACTTCAAGTTCAGATCAGCACCTCAGAACACCAAGTCTTGTTCCAAGGAATGTGTATGGAAGAGTTAAGGAAAAGGAACACATCATGAAATTGATGATGACAGAAGGCAGATCTGACAAATTAATTGTTGTGCCTATTGTAGGCATTGCAGGTGTTGGAAAGACAACTCTCACTCAACTTGTATACAATGATGTAGAAGTGGAAAGGCAATTTCACCATAGAATATGGGTTTGGGTGTCTCGCAACTTTGATGAAATGAGGCTCACAAGAGAGATGTTGAGCTTTGTTTCTCAAGAAAGACATGAAGGAATAGACTGCTTTGTGAAGCTTCAGGAGATCTTGAAAAGTTATGTTAAATCAAAGAGGATTTTACTTATTTTAGATGATGTTTGGGATGACAAGAACAATTACCAGTGGAACCAACTATTGGCTCCTTTTCGGCACGACAATGCTATTGGTAATGTGATTCTTGTGACAACTAGAAAATTGTCTGTTGCAAAAATGATTGGAACAACAAGACCAATTAAGTTAGGTGCATTGGAAAATGATGACTTCGAGTTATTGTTCAAATCATGTGCATTAGGTGATGGAAACTATGAATTTCCTGGAAATTTTAGCACAATTGGGCAGCACATAATAGAGAAGTTAAAGGGCAACCCCTTAGCAGCAATAACTACTGGGTCGCTATTAAGGGATCATCTTACCGCTGATCATTGGAGTAACATTCTCAAGAAAGAAAGTTGGAAGTCACTGGGAGTCAGTGGAGGCATCATGCCTGCTTTGAAGCTTAGTTATGATGAGCTACCATACCGTTTACAACAATGTTTCTCTTACTGTTCTATATTTCCTAACAAATATAGGTTTCTTGGTAAGGATTTAGTCTATATTTGGATTTCTCAGGGATTTGTGAATTGCACCCAAAATAAGAGATTGGAGGATACAGGGTGGGAATATCTGAATCAATTGGTAAACCTGGGATTCTTTCAACAAATTGAAGAACAACAAGAATTGGATGAGGAAGAAGAATTCTCTCTATGCCGTCAGATTTGGTACTCTATGTGTGATCTCATGCATGATTTTGCGAGGATGGTTTCAAGGACCAAATGTGCGACTATAGATGGTCCACAGTGCAATAAAATATTGCCAACTGTACAGCATTTGTCAATAGTAACCGGTTCTGCATACAACAAAGATCTGCACGGGAACATTCCTCGTAATGAGAAGTTTGAAGAACATCTGAGAAATTCAGTTACATCAGTTACCAAGTTGAGAACATTGGTTGTACTTGGAAAATTTGACTCTTCCTTTGTACAGTTGTTCCAAGATATATTCCAAAAGGCACAAAATTTACGCCTGCTACGAGTATCTTATCCACTTATCTGTTTCAAGTGCCTGAAGCATCCACCGGTTTTAATTCCTTCCTGTGCAGTTTGGCAAATCCTTTGCATCTTCGTTACCTAAAACTTGAGTTGGATGGGATTGTGCCACAAGTTTTGAGTACGTTTTTGCATCTTCAAGTATTAGATGTTGGATCAAGCATGGATACTTCTCTACCCAATGGCTTGTTGCATAATCTTGTTAGCCTGCGACATCTAGTTGCACACAAGAGAGTCCATTCTTCCATTACTAGCATTGGTAACATGACATCTATCCAGGAGCTACATGATTTTAAGGTTCGAATTTCTGGTGGCTTTGAGATAACACAACTCAAATACATGAACGAGCTTGTTCAACTTGGGGTGTCTCAGCTTGACAGTGTTAAAACCCGGGAGGAGGCTTATGGAGCAGGATTAAGAAACAAGGAACACTTAGAAGAGCTTCACTTGTCCTGGAAGGATGCATATTCAGAGTATGAGTTTGTCAGTGACACTAGATTTGAATCTTCTGCAAACATGGCAAGAGAAGTGATTGAGGGTCTTGAACCATACATGGATTTAAAACATCTACAAATATCTTGGTATAATGGTACCACTTCACCAGCTTGGCTTGCCAACAATATCTCAGTTACCTCATTGCAGTCGCTTCATCTTAATTATTGTGGAACATGGAGAACACTTCCATCTCTGGGAAGTCTTCCATTTCTTACAAAGCTGAAGTTGAGCAACATGTGGGAAGTAAAAGAAGTATTGATTCCTTCACTGGAGGAGCTAGTTTTGATCGACATGCCTAAGTTAGTGAGATGCTCAAGCACTTCTGTCGAGGGTCTGTGCTCCAGCTTAAGGGTACTGCAGATCAAATATTGTAAAGCATTGAAGGAGTTTGATCTGTTTGATAACGATGATAATTCTGGAATCACTCAGGGATCATGGCTGCCCGGTCTTAGGAATTTGATTCTGGATTATTACCCTCATTTGGAAGTGTTGAAGCCTCTTCCACCTTCAACTACGTGTTGTAAGGTACTCATCAGAGAAGTTCCAAGATTTCCGTATATGGAGGTATCATCTGGAGAAAAGTTAGAAATTGGGAATACTTATGGGTACAGAGGCGATGGTTTTGATGAATCTTCTGATGAATTGAGGATACTGGATGACAAAACTTTGGCATTCCATAACCTTGGAAACCTCAAATTGATGGAGATATATGGTTGCAGAAATCTAAGGTCTTTTTCGTTCGAAGGTTTTAGTCATCTTGTCTCTTTAGCAAGTTTGACAATAGTAGACTGCGAACAACTTTTCCCTTCAGATGTGTCGCCAGAGTATACCCTTGAGGATGTGACAGCTATGAACTGCAATGCCTTCCCATCTCTTAAAAGTCTCAGTATTCAGTCATGTGGAATAGCGGGGAAGTGGCTATCGTTGATGCTGCAACATGCGCCAGGCCTAGAGAAATTGGCTTTAGCAAATTGCGCCCATATAACAACAGTACTATTAACAACAGTATTGTCCGATGGAAGAGGAAGAAAACAGACTATTAACAACAGTACTGTCATCAGGAAATCCAGATGAGGCATTGACCTGGTTAGCTCGAGACTGACTCTTGCACGTTCAGTCACTCAAGATGATTGATATTTGGGACTGCCCCCGCCTAACATTTAACGGGGCCAAGGAATGCTTCTCTGGATTTACCTCCCTTGAGAAGCTAGTCATTCGAGGCTGCCCCGACCTGTTCTCGTCATTGGTACATAAAGACGTAACAGATGACCAGGCAAGCGGAAGATGGCTCCTCCCGAAATCACTTCAGGAACTTGAGATCGTTGAATATTCCCAAGAAAAGCTGCAGCTCTGCTTCCCTAGAGATATCACAAGCCTTAAAAAGTTAAATGTATATCACAGCCCAGGTTTGCAATCTCTACGGCTGCACTCATGCACGGCACTGGAAGAATTGGAGATTAGATGCTGTGGATCGCTCACCGTCACTGAACTAGAAGGCATACAACGCCTTGGCAGCCTCGGGCGTTTGAATGTATCAGACTGTCCTGGCTTGCCACCATGTTTGGAGAGCTTTTCAACGCTGTGCCCTCGGCTGGAAAGGCTTGAGATCGATGACCCATCTGTCCTTACCACGTCATTCTGCAAGCACCTCACCTCCCTGCAAAGACTACATCTTGGTCCCATGAAAATGACGAGACTCACAGATGAGCAAGAGCGGGCGCTTGTGCTGCTGAAGTCCCTGCAAGAGCTCGAATTCAATCGGTGTCGTGATCTCGTAGATCTTCCTGGGGGCCTGCACAACCTTCCTTCCCTCAAGAGGTTAAAGATATGGGATTGTCTGGGCATCTCAAGGCTGCCGGAAGCAGGTCTCCCATTTTCACTGGAAGAACTGGAAATCAATCATTGCAGCAAGGAACTAGCTGACCAATGCAGTCTGCTAGAAACAAGCAAGCGAAAAGTGAAAATTACTTTATGTACTCCAATTGATTACTGGCTGCTATGTTAAGCACATGTTTCTAAGCTGTCTCTGCTTTTGAGGAAATCTTCCGCCGTATACCCTCAGAGTTGACAGACCCTCATAAATGTGCAGTGTGCTCATTCCAGAATGAGCTGTCTCTGCAGGCATTCAATTAGGCTGCTCAACATATACTATCATGCAACAGGTAAACCGGCATGTTTCGCTGTTTGCTATTCATCTTGTCTTGTCAACTGAAAAATATAATTAATTTTCATTTCCTTGACTGCACAGAGAACTACTCCCTCCGTTCCTAAATATAAGTCTTTGTAGAGATTCCACTATAGACTACATACGGAGCAAAATGAGTGAATCTACGCTTAAAATGCATTTATATACATTCGTATGTGGTTCATACTAATATCTCTACAAAGACTTATATTTAGGAACGGAGGGAGTACACGAGATAAACCTGCAGATGTTTTATGTTGTTTGTTGCACAAGTTGTGTCCGAAATTTCCGCCATTCAGATATGCTCTGCAGCTACAACAATGCACCTTTTCAAGGAAAAAAAAGCTAAAACAAAGCACTTCAGAGACAGGAATAGTAGCTCTCGTCTGACACGAGAAGGAGGATATGTGGGGTTACTCTTAACTAAATTCATGTGTTGATCAGCCAGACTCAGAAGTCAGGATGGCCTCGGCAGACGCCTAATGTGTGCAAGAATGATTAAAGTTGGATATGCAAGCCTGTAACCTGGTGTGCCGTCGCCGATTACTAGTTTCCTGTTGTGATATCAGCGACGCAGTGTGTGTGTAGTATACTACTATGCTATCTTGGTACATCCTAATGAGCTCATCTCTTCCCATTTTCCTTTATCTTTGTGATGCTTCAAACTATCTTTGTGATGCAGTGTGTCTGTACTATCCTATCTTGGATCTTCACAGAATTTTGCTACTGGTCTGGACTCATTCTGTCAGTGGTTGTTTGCTTTGTGGACTTGTGCTCGTGGTCTCTGTTTTTTCAAGCTGATCCTGAAGCTTGCTGGAGCCTGTGAGGCACGATAAAAATTCTCATCAAAGTGAGGCACAATAAAGCTCCTCGTTTCTTGTTGACTGTACGAGCTCCTTTCTCCAGTGTGTAACTGAAAATGGGACGAGAATGCCGAAGGTTTGCTCATAAGGTCATATCACCATGCGAAACCCCAACAGTAACGTCGGGGAAACAGAGTTGATATGGCCTCCTGTAAGAAAAAAGAGCTGGTACGGCCCGCTCCAGTTTCATCATTTCATTGCCATCCCTCGCATGTGTAGCGCTGTATCGGAGGAGCTCTCCTCTTTTGCGTGATATATTGCGTTATCAATAAGAAAACTATTCATGTCTTTGCTTCGGATATTTTTATGTATCTGAATTTTCTTGATCAGAAGAAAACTCTTTTTACTCTGTTTGTGATGCTGGACAAGTCATGCTGTCTTCGAACTGTGCATGAATAATTTTGCTCCTGATCTGGAGCACTTACATCGAGTGGTAGCTTACTTTGATGTGTGCACTAACAAAAGATTAGAAAATGTACATTATACCTGATGGCGTAATCAATCTTTTCTGTTGTGCTCAAGTTGTTGTCGATCATGCTTATCGTTTTCAGACTTCCTGAGCTGGCCGGCCTGTGAATGTGGTAAGCAAACAAATTTTCTAGTCAATGATATATAGGCACAAGTAAAGAACAGGACAAGTTAACTGAATCCAAGGCAACCTGCACATCTCAGAAACAAGTACTCACTCAAATCATACTGTTCAAGTAAGACGCTACAGGAAGTTAAGCTGCCCATCGTCTTAAACCAGCATAGGATGCTCCCTTAACTCAAAATAAAGCTGTTAAAACAAGCTCCTCTGCAATGCAAGAACTTCATCAGTTCATGGAGAATAAACAGGGAGCTCGACAGTACCGCAGGATGACGAGGAGCCACTGCCCACCAGAGATTGGTAAGTTGCGGTTGGATCTGGCCACAGCGCCTCCGCATCGGCGCCCAGAGGTTGGTCGGATGGGGGATGTTGGCGAGCTCGCCTGCGAGGCGTTCCCTGAGCGCACTGCCATCACGGCGGGCCAGCCCCCGCTTGCAGGAACGTCGGGCATCCCGGGCGGCGGCGTCTTGCAACTATCGGCGCGTGGCGTGGGAGGGCAAGCCTGAAGAAGACAAACTAGCTAAATGGGCCGGACATTGGCACAGGCCATTGGCGCATATATTTTTATATTTTCCCAAAAAGTATACATATTAAAAATATATTCAGTAATCACTTTATATTTCTCAAAAAAATAATCAATTTA >curated_TraesCS2B01G734100LC_Ta_2B12MAASIGWLVETISATLKIDKLDAWIRQVGLADDIQKIKSEIWKVQTVVTTLLPRVRGSQTSFWMKLSLFSKSGSMKPTILSTSSTTTGSNTKSKVCLPLQIQASYSEEDAIAFLLHDFFWHSYKGTGEPTVIGSSSSRKGKRRRSKAWELFDVIQEVWEQPMKARCKYCPTEIKCGPTSGTAGMLNHSKICIPGLNNQPPNPSSTSDANANVTPITAANTVTPWDMAELSNKIKKIAGQLQYIGREVGEILKLHGSDCTSSSDQHLRTPSLVPRNVYGRVKEKEHIMKLMMTEGRSDKLIVVPIVGIAGVGKTTLTQLVYNDVEVERQFHHRIWVWVSRNFDEMRLTREMLSFVSQERHEGIDCFVKLQEILKSYVKSKRILLILDDVWDDKNNYQWNQLLAPFRHDNAIGNVILVTTRKLSVAKMIGTTRPIKLGALENDDFELLFKSCALGDGNYEFPGNFSTIGQHIIEKLKGNPLAAITTGSLLRDHLTADHWSNILKKESWKSLGVSGGIMPALKLSYDELPYRLQQCFSYCSIFPNKYRFLVLDVGSSMDTSLPNGLLHNLVSLRHLVAHKRVHSSITSIGNMTSIQELHDFKVRISGGFEITQLKYMNELVQLGVSQLDSVKTREEAYGAGLRNKEHLEELHLSWKDAYSEYEFVSDTRFESSANMAREVIEGLEPYMDLKHLQISWYNGTTSPAWLANNISVTSLQSLHLNYCGTWRTLPSLGSLPFLTKLKLSNMWEVKEVLIPSLEELVLIDMPKLVRCSSTSVEGLCSSLRVLQIKYCKALKEFDLFDNDDNSGITQGSWLPGLRNLILDYYPHLEVLKPLPPSTTCCKVLIREVPRFPYMEVSSGEKLEIGNTYGYRGDGFDESSDELRILDDKTLAFHNLGNLKLMEIYGCRNLRSFSFEGFSHLVSLASLTIVDCEQLFPSDVSPEYTLEDVTAMNCNAFPSLKSLSIQSCGIAGKWLSLMLQHAPGLEKLALANCAHITTSLKMIDIWDCPRLTFNGAKECFSGFTSLEKLVIRGCPDLFSSLVHKDVTDDQASGRWLLPKSLQELEIVEYSQEKLQLCFPRDITSLKKLNVYHSPGLQSLRLHSCTALEELEIRCCGSLTVTELEGIQPLGSLGRLNVSDCPGLPPCLESFSTLCPRLERLEIDDPSVLTTSFCKHLTSLQRLHLGPMKMTRLTDEQERALVLLKSLQELEFNRCRDLVDLPGGLHNLPSLKRLKIWDCLGISRLPEAGLPFSLEELEINHCSKELADQCSLLETSKRKCAHSRMSCLCRHSIRLLNIYYHATARLRSQDGLGRRLIVSVLSYLGSSQNFATGLDSFCQWLFALWTCARGLCFFKLILKLAGAYFLSWPACECAVKTSSSAMQELHQFMENKQGARQYRRMTRSHCPPEIGKLRLDLATAPPHRRPEVGRMGDVGELACEAFPERTAITAGQPPLAGTSGIPGGGVLQLSARGVGGQA- >curated_TraesCS2B01G489400_Ta_2913ATGTTGCTCGGAATCTTCGAAACAGCTGAGCAGGCCGCGAGAACCTACGATGCGGCGGCGCTGCGCTTCAAGGGCGCCAAGGCCAAGCTCAACTACCCCGAGGGTTTCCAGGGACGCACCGACCTCGGCTTCAAAGTCACCCGCAGCATACCGGACGGATTACAACAACATCGCCACTACCCCTCCACCATGGAGGCGCCAGCAACGCAGCCGTCGCCGCAACAGCAGCCGACCGTCCCAGTCCTCATGCGGCACGAACTGCCGCCTCAGGGCGCCGGCAGCTCCAGGGGCGCTGTCAACCTGCCCTTCGGCGCCATGTCGGCCCCGTCCACGTCGTCCACCTCATCGCCGCACATGCTCGTCCCTCCGCTTGCGTCCGAGGACCATACAATGAGAAGAACTGTAAGTGTAGAAGAGGAAGCTAACGACACACATGACGGAGTGACGGCGCGCACACAATCTAGCAAGTTTGTGAACAGTTTTTACGGTTTTGCAAGTGCGTGTGCATTCTTTACTTTATCTGACTCTGGTCAAAGGACGACCCTTTTTCTTTTTCTTTTGGCAGTTGCAAGGAACAACGCCGAATGTATGCACGGTGCAGACAGGGTCGATGAGATATCAAGGGGCGATGCTGACACACCGAGTAACATTGTTGGCAAATTGCGGTCCGTCGTATGGGAACACTTTACGATCACAGAAAAAGATAATGGAAAACCGCTCAAAGCAGTATGTAGACACTGTGGCAATGAGTTTAAGTGTGATACAAAGACCAACGGTACATCGTCTATGAAAAAACATTTGGAGAACGAGCATGCCGTGACCTTTACCAAGAAACCTCCTAGAGGGCGTCCACCAAACCCTTCAAGGTACCCTCCCAAAAGAGAATTGGGCATATACCTTGCATGAGCATATTTTTAGAAACTCGTTAATACACATCTGCTTCGGGAGCCCGATAATTGTGGTCCTAATAGCCAACCTAATGTCTCATTTTCTTACTGCAGCACTAGTGAGCCTATCTTAATCGGCAACTCGTCCAGGACAAAAGGAAAGAGACGATGGTCCAAGGCATGGCAACTTTTTGATATCATAGAAGAAGAAAACGGAGAGCCTATCAAAGCAATATGTAAATATTGTCCAACAAAGATCAAGTGTGGACCAATGTGTGGGACAGCTGGTATGCTCAACCATAACAAGATTTGTAAGAACAAACCTGGACCATATGACCAGTCACCAAACCCATCAAGGTAGCTAATGAATCTATACCTTGCATCGACACATTTTTACAAGTCATTTAATTAAGAGGTCTCACCGTGGTTCTAGTAGCCAATTCACGGTTTCTTACATTAATTGCTGCAGCACGGGTGATGCTACTGCACATGTGAAGCCTTCATCTAGCAGAAAAAGGAGGAGACCCGAATCAACACAAATGACCGCGCCTAACACCGCGACTGGTTGGGACAAGGTCGAGATATCCAATAGGATACAAAACATAACTAGTGAGCTACAAGGCATCCAACTGGAAGTGCCTAAGGCTTTCTATCCATGTGGATCAAGCTTATCTTCAAATTCAGATCACCACCAGAGTACAATCTCAGATCAGCGCCTAAAGACATCAAGTCTTGTTCAAAAGAAAGTGTATGGGAGAGATGTAGAAAAGAACTCCATCGTGAAGTTGGTGAGGGCAAAAAACAAATCTCACGGTGTAACTATTTTGCCTATTGTAGGGATTGCGGGCGTTGGAAAGACAACTCTCGCTCAACTTGTATACAATGATCCATATAGTGAAAGTCAATTTGATCACAAGATATGGGTTTGGGTGTCTCACAACTTTGATGGCATGAGGCTCACAAGAGAAATGTTGACCTCTGTTTCTCAACAAAGGCATGAAGGAATAGACTGCTTTGTGAAGCTTCAGGAGATCTTAAAAAGTCATATCAAATCAAAGAGGGTTTTACTAATTTTAGATGACGTCTGGGATGACAAGGATGATTGCCGCTTGAACCAACTAATGGCTCCTTTTAAGAATGATAGTGATAATGGCAATGTGATTCTTGTGACAACTAGAAAACTTTCTGCTGCAAAAATGATTGGAACAACGGAGCCAATTAAGTTAGGTGCTTTAGAAAAGGATGACCTCTGGTTATTGTTCAAATCATGTGCATTTGGTGATGAAAACTATGACTGTCTTGGAAATATTAGCACAATTGGACGACAAATAGCAGAGAAGTTAGAAGGCAACCCGTTGGTAGCAGTAACTACAGGGGCACTATTAAGAGGTCATCTTACCGTTGATCATTGGAGTAACATTCTCAAGAAAGAAAGTTGGAAATCACTGGGACTCAATGGAGGCATCATGCCTGCTTTGAAGCTTAGTTATGATGAGTTGCCACACCATTTACAACAATGTCTCTCACATTGTTCTATATTTCCCAAAAAATATAGGTTTCTTGGTAAGGATTTAGTCTATATTTGGATTTCTCAGGGATTCGTGGATCGCACCCATTTAAGTGAGAGATTGGAGGAGGCAGGATTGGAATATTTGAATGATTTGATGAGCCTGGGATTCTTTCAGCAAGTTGAAGACCAGCAGGATGAAGATGGGGATGAGGATGAGGAAGAAGAATCCTCTCTAGGCAGTCAAATTCGGTACTCTATGTGTGGTCTCATGCATGATTTTGCCAAGATGGTTTCAAGGACTGAATGTGCAACTATAGATGGTCTACACTGCAAAATGCTGCCAAATATACGTCATTTGGCGATAGTAACTGATTCTGCATACAACAAAGATTGGTATGGGAACATTCCTCGTAATGAGAATTTTGAAGAAAATCTGAGAAACACGGTTACATCGGTCAGCAAATTGAGGACGCTGGTTTTAGTTGGGCACTATGACTCTTTCTTCATAGAATTGTTCCAAACTATATTCCGAAAGGCACATAATTTACGCCTGCTGCAAGTGTCTGCAACATCCACTGGTTTTAACTCCTTTTGTTGTGTTTTGGCAAATCCTTTGCATCTACGTTATCTAAAACTTGAGTTGCACGGGGTTGTGCCACAAGTTTTGAGTAAGTCCTTTCATCTTCAAGTATTAGATGTTGGCTCAGACATGAATACTTCTGTACCCAATGGCATGCATAATCTTGTCAGCCTGCGCCATCTTATTGCACGCAACAGAGTGCGCTCTTCAATTGCTAGCATTGGCATCATGGCATCTCTTCAGGAGCTACATGATTTTGAGGTTCGAAATGCTAGCGGCTTTGAGATAACACAACTCCAATCCATGAACGAGCTTGTACAACTTGGGGTGTCTCAACTTGATAATGTTAAAACTCGGGATGACGCTTATAGGGCAGGACTAAGAAACAAAGAACACTTAGAAGAGCTTCATTTGTCCTGGAAGTATGCACTGTTAGAAAATGAATATAGCAGTGAAAAGGCAAGAGAAGTTCTTGAGGGTCTTGAACCACATATGGGTTTAAAGCATCTACAAATATCTAAGTATAATGGTACTACTTCACCAACTTGGCTTGCCAACAAAATCTCGGTTACCTCCTTGCAGACACTTCATCTTGATGATTGTCGTGGATGGAGAATACTTCCATCTCTGGGAAGTCTTCCATTTCTTACAAAGCTGAAGTTGAGCACCATGTGTGAAGTAATAGAAGTATTACTTCCTTCACTAGAGGACTTGGTACTAATTAACATGCCAAAGTTAGAGAGATGCTCAAGCACTTCTGTGGAGGGTTTGAGCTCTAACTTGAGGGTGCTGCAGATCGAGCATTGCAAAGCACTAACGTCATTTGATCTGCTTGAGAATAATGATAAATTCAAAATCGAGCAGAGCTCGTGCTTGGCTGGTCTTAGGAAATTAATTTTGTATGATTGCCCTCGTTTGAAAGTGTTGAACCCTCTTCCACCTTCAACAACATGTTCCGAGTTACTCATCAGTGGAGTTTCAATACTTCCGAGTATGAAGGGATCATCAAGTGATAATTTACGTATTGGGCTCATTAATGAGTCTATAATCTATGGCAGTATTGATGGATACGCTGATGAGTCGAGGATAATGGATGACAAAATTTTTGCGTTCCATAATCTTAGAAACCTCAAATCGATGGTGATATTTGGTTGCCAAAATTTAAGGTCATTTTCATTTGAAGATTTTAGTCATCTCAGCTCTTTAAAGAATTTGGAAATATCAATGTGCAAGGAACTTTTCTCTTCAGATGTGATGCCAGAGCATACCCTTCAAAATGTGGCAACCACGAAATGCAGGGCCTTCCCATCTCTTGAAAGTCTCAGTATTAGGTCATGTGGAATAACAGGGAAGTGGGTATCTTTGATGCTCCAACATGCGTGGATCCTTGAGGAATTGAGTTTGGAAGATTGCCTACACACAACAATAATACAATTGCCGACGGAAGAGGAAGAAAACAGTCTATCAGATCTTATCTCAGCCAGGGAGGACTCATCATCAGGAGATCAAGACACATTGACCTGGTTAGCTCGAGATAGACTCTTGCACATTCCATCAAATATCACCTCCTCTCTCAAGTGGTTAACCATTTGGAAGTGCCGTGGTGTAACATTTAATGGGAGTGAAAAAGGTTTCTCCAGATTTACCTCCCTTAAGGAGCTACAAATTAGGGGATGCCCCGAGCTAGTCTTGCATTTGGTGGATAAAGATGGAACTTATTACTGCACGAACGGAAGATGGTTCCTCCCATCATCACTTGAGGTACTGGGCATCGACAACTATTTCCAAGAAAAGCTTCAACCCTGCTTTCTGAATGATCTCACCAGCCTTAAAAGGTTATCCGTCTCGTCCAGGCCATGGTTGAAATCTCTACAGCTGCACTCATGCACAGCACTAGAAGAGTTGAAAGTCATTCAGTGTGAATCGCTCACGACACTAGAGGGCTTGCAATTCCTTGGCACCCTCAGGCATTTGACAGTATACGACTGCCCTGGCATGTCTACCTGTTTGAAGAGCCTTTCATGGCGCTACGGGCTATGCTCTCGGCTGGAAACGCTCGGAATTGGTGATCCATCAGTCCTTACCACATCATTCTGCAAGCTCCTCACATCGCTGCAATGCCTAAAATTATATCATTTTGGGTGGGAAGTAACGAGGCTAACCGATAACCAAGAGATAGCCCTTGTGTTCCTCAAGTCCCTGCAAGAGCTCCACTTTTTGTGCTGTTATGATCTAGTAGATCTTCCTGCGGGGCTGCACAACCTTCCTTCCCTCAAGAAGTTGAAAATAGACACTTGTCCGCGCGTCTCAAGGCTGCCGAAAACAGGTCTCCCACTTCCGCTGGAAGAACTGGAAATCGAGTTTTGCAGCAAGAAGCTGGCTGATCAATGCAGGCTGCTAGAAACAAGCAAGCTAAAAGTCAAAATTAGTCTATGCTCTTGA >curated_TraesCS2B01G489400MLLGIFETAEQAARTYDAAALRFKGAKAKLNYPEGFQGRTDLGFKVTRSIPDGLQQHRHYPSTMEAPATQPSPQQQPTVPVLMRHELPPQGAGSSRGAVNLPFGAMSAPSTSSTSSPHMLVPPLASEDHTMRRTVSVEEEANDTHDGVTARTQSSKFVNSFYGFASACAFFTLSDSGQRTTLFLFLLAVARNNAECMHGADRVDEISRGDADTPSNIVGKLRSVVWEHFTITEKDNGKPLKAVCRHCGNEFKCDTKTNGTSSMKKHLENEHAVTFTKKPPRGRPPNPSSTSEPILIGNSSRTKGKRRWSKAWQLFDIIEEENGEPIKAICKYCPTKIKCGPMCGTAGMLNHNKICKNKPGPYDQSPNPSSTGDATAHVKPSSSRKRRRPESTQMTAPNTATGWDKVEISNRIQNITSELQGIQLEVPKAFYPCGSSLSSNSDHHQSTISDQRLKTSSLVQKKVYGRDVEKNSIVKLVRAKNKSHGVTILPIVGIAGVGKTTLAQLVYNDPYSESQFDHKIWVWVSHNFDGMRLTREMLTSVSQQRHEGIDCFVKLQEILKSHIKSKRVLLILDDVWDDKDDCRLNQLMAPFKNDSDNGNVILVTTRKLSAAKMIGTTEPIKLGALEKDDLWLLFKSCAFGDENYDCLGNISTIGRQIAEKLEGNPLVAVTTGALLRGHLTVDHWSNILKKESWKSLGLNGGIMPALKLSYDELPHHLQQCLSHCSIFPKKYRFLGKDLVYIWISQGFVDRTHLSERLEEAGLEYLNDLMSLGFFQQVEDQQDEDGDEDEEEESSLGSQIRYSMCGLMHDFAKMVSRTECATIDGLHCKMLPNIRHLAIVTDSAYNKDWYGNIPRNENFEENLRNTVTSVSKLRTLVLVGHYDSFFIELFQTIFRKAHNLRLLQVSATSTGFNSFCCVLANPLHLRYLKLELHGVVPQVLSKSFHLQVLDVGSDMNTSVPNGMHNLVSLRHLIARNRVRSSIASIGIMASLQELHDFEVRNASGFEITQLQSMNELVQLGVSQLDNVKTRDDAYRAGLRNKEHLEELHLSWKYALLENEYSSEKAREVLEGLEPHMGLKHLQISKYNGTTSPTWLANKISVTSLQTLHLDDCRGWRILPSLGSLPFLTKLKLSTMCEVIEVLLPSLEDLVLINMPKLERCSSTSVEGLSSNLRVLQIEHCKALTSFDLLENNDKFRIEQSSCLAGLRKLILYDCPRLKVLNPLPPSTTCSELLISGVSILPSMKGSSSDNLRIGLINESIIYGSIDGYADESRIMDDKIFAFHNLRNLKSMVIFGCQNLRSFSFEDFSHLSSLKNLEISMCKELFSSDVMPEHTLQNVATTKCRAFPSLESLSIRSCGITGKWVSLMLQHAWILEELSLEDCLHTTIIQLPTEEEENSLSDLISAREDSSSGDQDTLTWLARDRLLHIPSNITSSLKWLTIWKCRGVTFNGSEKGFSRFTSLKELQIRGCPELVLHLVDKDGTYYCTNGRWFLPSSLEVLGIDNYFQEKLQPCFLNDLTSLKRLSVSSRPWLKSLQLHSCTALEELKVIQCESLTTLEGLQFLGTLRHLTVYDCPGMSTCLKSLSWRYGLCSRLETLGIGDPSVLTTSFCKLLTSLQCLKLYHFGWEVTRLTDNQEIALVFLKSLQELHFLCCYDLVDLPAGLHNLPSLKKLKIDTCPRVSRLPKTGLPLPLEELEIEFCSKKLADQCRLLETSKLKVKISLCS- >curated_TraesCS2D01G466600TACTGTTGTACAGTTGTACTTTCCCCCCATTTGATGGAGGCCGCGATCGCGTGGCTGGTGGAGACCATCCTTGCAACACTCCTGATCGACAAGCTTGATGCTTGGATTCGCCAAGCCGGGCTTGCCGATGACATCGAGAAGCTCAAGTCGGAGATCAGGAGAATCAAGATGGTGATCTCTGCTCTCAAGGGCAGAGGGATCCGGAAAGAGGCACTGGCTGAATCTCTCGCCCTTCTGGAGGATCACCTCTACGTACGACGCCGGCGACGTGGTGGACGAGCTCGACTACTACAGGCTCCAACAGCAGGTCCGGGGACAAGGGGGCACTCCCACTGCCTGGCCGCCTGCAGATCCAAGCGTGCATGGTACGCGTACTAGTGCTCGTAGATCCAAATCAAAGTGTACTAATTATTACTAGTTCGGTCTAATATATCTTGCTTCAAAAGACAAATTGATCTTATCTTATCAAGAATATGCATTTCTTTCCTGGGCATGTGTTTTTGGGCACAGTTGCAAGCGACGAGCGGCAAGGTGTGGATGGAGCCGAGCGAGTCAATGAGATACCGAGGGGCGATGCTGCTACACGTAATAGCAGTGTTGGCAAATTACGGTCGCTCGTATGGGAGCACTTCACGATCACACAAAAGGATGACGGAAAGCCTGTGAAAGCAAAATGTACATACTGTACAGAAGAGTTCAGATGCGAAACAAAGACGAATGGCACGTCATCTATGAGGAACCATTTGGAGAAAGAGCATTCCGTGATTTGTACGAAGAGACCTGGAGCGCATCCACCAAATCTTTCAAGGTACCTTCAAAAGGACTTTTGTTTTTCGAAAATGAGGTTGAATCTTCTGTCTCTGCATTAAGCCATGCACACGGCCATTTTATTATATTATTCAAAAATGCCTTATACAAGATACTAAAACTTTGATCCTTCAGAATCCATCTTCTAGACGATAAAAGTCGCACCACCTACAAGCTTGAGGATAATGGTGGTCATGATCAGGGCCACATGCCCTGACCTCACCCCTACACAAATCATCCAAAACCGGAACGCCGGTCCAGCGGACCCTTAGCGCATCACATGCGTACACTCCGAAAGTCGCCACCGCCGCCTTTTGCGAACCCATCTTCGATGTAGGGATCAATGAAAAGACCTTGTCAGGTATGCCGTTGACGCCACCGCGAAGCCAGACCGCGTCACCGCCCTGCACGCGTCCATCATCGAGAGTCCGCCGCCGAGACTTGTCGTCTTCGACTCGTAAGACCACACAACTCCACCTCAGGATCCCTTCGGCCAGCACATGCTCCAGAAAAACGATGCCTCGGGAGGGTAAACGGCTCCGCGCGCCGCTATCATCCGATCCGGGAGACCCGGATCTAGGGTTTCTCCCAGTGCGGCCTGGGCGGGAAGACAACAACTACATCAATGATGCCTCTAACAAGAAAATGACGCCGTCATCGTCCGCCATGACGGAAGTCGGCGCATTTTTACGGGTAGCCTCACCTCCTCGAACCCATGGCTGGCTTCCGATCCACAAATCCCGGAGGGTTGCGGATCTCCCACATCAAGCGTCGTAGACGCCGGAGAAAACTCCGGCCGCCACACGCCTCCAGCAACGAACTCGGGTATATGATCCCTTGATCCACCGCCCCCGACACAGCCACGTGAAGCTGTCTCCTGGCCCGTCATCCCCGCCAGAGGGGCCGCTGCCGCCGCCGTGTCCGGAGCCACCGCTCCAGGGCCCCTGCGCCGTAGATTGCTCACTAGAATTAATTGCATTGTGAGATTTTTGTTAGTATACTTTGTGTTGTTGTTTGATCGCGATTCTTCTGCTCTGTGTTCTCATCTTTGCTAGTAGTATACACATACAAGGAATTGATTTTTGCGAGAACTATAAAGTGCAGGTTCCGAAAGCGTTTTCATTGGGATCGATCTAACCACACTGGTAACAATGATTGACCACAGACTGCTCGGGCTTCATGCCGGGCCTTGGGCTTCGGGCTTTCATGCCGGGCCAGACTCGGGCTTGCATTTAGACAAAATGTCAGGCTTCATGGTCAGGCTCGGGCTTGAGATATGACGGTCGGGCTTTTTAAAGCTGAGCCCAAAACCCGGCCCGGCCCGGCCCAAGGTATGCCCAGGTTTGCCGCCCAGTCTCAGTGTATAGTTGTAAAAAAGAGCCTGAATCAGATGTAACAGCATGGTCTGTAGTAGTGATATATCTTCCAGGGGCCCTTTTACAACACAAAAATTGTGTGTGCTGCCTTTAAATGCCCACTACTTGGGATCGTGCATATAGCTCTGCTTACCACACTCATTGCGTATAATATGTTAGCTCTTGTGTGCCACAAATAGATGAATCGACCTACAGGCTACAGGACGCTAGTATGGATCTCCTGATCCAGTGTGGTGTTGATAGCTCTCTCTATCAACAGGATCTCCTGATTTATCACAACTACAGATTTTGCTCTACTGAAACTGAAACAACCCGACACCCAAGCATATGGTCTTGCTGAGGGGTCAAATGCATACCCTCATCGAGAGAGAACTGAACCTTTGGGAGATCTTGGAATCTTAATGCCACCAAAAAAATACTTGAGTTGACCCAAATTCTTAACCTCAAATCTGTTGCTAAACCTCACCTTCAGGCGACTTACCTCCACATTTACATCTCCCATGATAATAATATTGTCCACATTAATAACAAGGATGTTAATTTGTTTCTTAATGCTGACATAATATCGTATGATCTCCATTTCATTGTTTGTGGCTCACCGAAACCTGTCAAACCTCGCTCTTTGTAACTGCTTGTGACCTCCCGCAAAAAAAAAACTGCTTGTGACCTCCCGCAAAAAAAAAACTGCTTGTGACCATACAAAGACTTCTTCAATTTGCACACCTTTCCATTGGTTCTGGGGTACTAAAACTAGACGGGGTCTCCAAATAACGCTCCATGCAGATATGCATTCTTGACATCCAAGTATCCAACTGATCCAATGGCCAACCAAAGTTAGCGGTGCAAGAAATAAGTGATCTTTTTTGCGAGAAAATTTTCAATCTATTCATTTTCAATCATGCAGTACAACGAATACCAGAAATAATAGAAATTACATCCAGATCTGTAGACCACCTAGTGACGACTACCAACACTGACGCGAGCTGAAGGCGCGCCGCTGTCATCGCCCCTCCATTGGCGGAGTTGGGCACAACTTGTTGTAGTAGACAGCCGGGAAGTCGTCGTGCTAAGACCCCGTAGGACCAGCGCACCAGAACAGCAGTCGCCGCAGCTGAAGAATAACGTAGACCAGAAGGATCCAATCCGAAGACACACGAACGTAGACGAACAACGACGAGATCCGAGCAAATCCACCAAAGATAGATCCGCCGGAGACACACCTCCACACGCCCACCAACGGTGCTAGACGCACTGCCGGAAGGGGGCTAGGCGGGGAGACCTTTATTCCATCTTCAGGAAGCCGATGCCGTCTCGTCTTCCTTAGCAGGAACAAACCCTAGCAAAACTGAAAGAAACGACTAAAAACGGATCCCTCCCGCCGGCCCTTGCCGAGATCCACCGCGCCCCTAGGGCCATCGGAGAGGAGGCGGACCTGCGGCGGCGTCGGCGCGAGGCAGAAACCCCAACTTTTTTGTGGAGGAGGAGGAGGCGGCTAGAAAGGCTTCCGTGTCCGTAATAGTCAATCCCATAGATTTATGGACTTGGAATGTGTTTGGTTGACATCTTTGTTTTTGAGCATTTTGCATACTTTTCCCAGTTGAGCCTGTTTGAGCTAATGCATGCAAAAAACCAACATCTGCATGTAGTTTGGTTGCCTACATTTAGGCTACCTGCATCAGGGAAGCAATTTTTACCATGGTATTTGGTTGCTTGCATCGCAGTTGTTAGACAAACTACATGCTGTTAATTTGGTTGCAAATGGCATAAGGTCTGATCACTTCTCACTAGTGATGACCTTGCCACACACGGGTTGAACATTGCCTCGGTCCTAACTTGGAAAGATATGGCAATTTATCCTAGCTACTAACAAATAGCATACAAATTAAGAGCCATATGCCTGAATAAGGGAAAGTTCATCGATGCTAAATAGGGTGAAGTCCATCCTCATCCTTTGTTCTTCCAGGCTTCGCTGTCAAATGCCTCCACACCATGACTGGAGCTGACAACATCATCAGGCTTCACATCTTTCTCCTCCAGCACAAGTTCATCACAACCTCATTGTAGGATCCAGTTATGAAGGATGCAACATGCAAGAACAAGTTTACCCTGGGTAGGGTAAGGGTGAAATGACTTTTGATCCAGGATCTTAAACATATTCTTCATAGCTCTAAATGCCCTCTCAACCATAACTCTAAGGCTGGAGTATCAGAGATTAAAAAGTTTATGTGGAGTCGTAGGATAGTTTCTACCAGAGAACTCGTTCAGATGGTACCTGGTTTTCCTGAGAGGTGGAAGAGCACCCGGCCGACATGCATAGCCAACATCTCCTAGGTAGAACTTGCCATCGGGGATATTGATGCCATCAGGTCTACTCATGTTGTCACTTAGAATGTTAGCATCAGTGCTGATCCTTCCCAACCAGCTAGCACATATGTGAACTTCAGATCGAAGTCAACAGCACCAAGAACATTCTGGCTTATAGAAGAAATTAGTGGTGTTGGTATTACCCTTATAGAAGAAAGAAAATGAAACAACAATTAAAAACAAATGATGAAAAACTTGCACACAGTTTGTACTGAAATTGCATATTTTTATGAATGCAAAAATAGGCAGATAATAATGCAATTTTGCACTACAGTATAATTTATACACATTGTATAATACTTTTGTATATATTTACACACGCACACCTAATATTTACACATACGCATAAAGAAAAAGAAAAACTGACTAGAAATACTTGATAAACAATAATAAATACTAAAACTAGTACGAAGCTAAAAGACAAAAACTGAATTTTCCCTAAGGTAGAATGAATTAGGTGCATTGGTTTCCCCTCTAAAAAAGAAATAAAGAAAACTTGAAACAGACGACAATAGAAAATTTTGCACATGAAATGCGCGGTTGCACAATATGCAAAAACAAGTATACCGTAATTTTCAGATAACAAAGACACATGCATGTGCATACATGCACATGGCTGCAATGCACGAAGAGCATACACAAAGTCACTCACAACACCAGCACCAGCACATGCAGGTCCCTTGCAAGCAGGCAAGACACACACATGCACGCACACAAAATCTGACACATAAGAAAAGAAAAAAACAGACAAAATATTTAGTAGAAGAAAAGAGTGACTGACCCAAAAGTAAATTTCAGAAGACTTAAATGTAGCAAAACTGATATACATCAGCTTGAGAGCCCATGGTTTTCCTAATAGCCAGCCCACCATCTTTTTCTGACTGCAGCACCGGCGAGCCTATTGTAATTGGCAGCTCATCCAAGGGAAAAGGAAAGAAACGACGGTCCAAGGCATGGGATTCTTTTGATGTCATAAAAGAAGTAAACGGACAGCCTATCAAAGCAAGATGTAAATACTGTCCCACAGAGATCAAGTGCGGAACCGGGAACGGGACAGCAGGTATGCTCAACCATAACAAGATTTGTAAGAAGAAACCTGGACTAGATGACCAGCCACCAAACTCGTCAAGGTAGCTGATGAATCTTTGCACCGTGACATTTTTAGGGGGTTGTTTAAATAAGAGCCCCATTGTGGTTCTATTTTCCAATTGACGGTCTCTTCCTTACTGCAGCACCAATGATACTACCGCAAATGATGCTACCACAAATGCAAGGCCTAATCTAATTGGTGATTCATCTAGCAGAAAAAGAAGGAGAGTTGATGAGGAATCCGCACAAAATATCGCAGCTAACACAAGTACCCCTTGGAACAAGGCTGAATTATCAAACAGAATACAACAAATAATTAGTCGGTTACAGGACATCCGAGGGGAAGTGAGTGAGGTTTTCAAGCTACATGAATCAGACTCTGCTTCAAGTTTAGATCACAACCGGAGTACAACCTCGGATCAGCATCTGAGAACATCAAGTCTTATTTCAAGGCAATTGTATGGGAGAGTTGCAGAAAAGAAATCCATCTTGAAGTTGATGATGTCAGATGACACATCTAATAGCATAATTGTTCTGCCTATTGTAGGCGTTGCAGGTGTTGGAAAGACAGCTCTCACTCAACTTGTATACAATGAACCAAACGTGGAGAGTCGATTTCAGCACAGGGTATGGATTTGGGTGTCTCGAAACTTTGATGAAGTGAGGATAACAAGGGAGATGTTAAACTTTGTTTCTAGAGAAAAACATGAAGAAATAAACTGCTTTGTGAAGCTTCAGGAGATCTTGAAAATTCATGTAAAATCAAAGAGGGTTTTAATAATTTTAGATGATGTCTGGGATGACATGAACGACTGCCGATGGAACCAATTGTTGGCTCCTTTTAAGTTTAATAGTGCTAATGGCAATGTGATTCTTGTGACAACAAGAAAACTATCTGTTGCAAAAATGGTTGGAACAACTGAGCCAATTAAGATAGGTGCTTTGGAAGAGGACGATTTCTGGTTATTGTTTAAATCATGTGCACTTGGTGATAGAGCCTCTGAAAATCCTGGAAATCTATGCACTATTGGACGACAAATAGCAGGCAAGTTAAAGGGCAATCCGTTAGCAGCAGTAACTGCAGGGGCACTATTACGAGATCATCTTACTGTTGATCATTGGAGTAACATTCTCAAGAAAGAAGACTGGAAATCGTTGGGTCTCAGCGGAGGCATCATGCCTGCTTTGAAGCTTAGCTATGATGAACTGCCATACCATTTACAAAGATGCCTATCATATTGTTCTATATTTCCTAACAAGCATAAGTTCTCGGGTAAGGATTTGGTTTATATATGGATTTCCCAAGGATTTGTGAGTTGCGCCAATTTAAGTAAGAGCTTGGAGGAGATAGGATGGCAATATTTAATTGATATGACGAACATGGGCTTATTTCAGCAAGTCAGAGGAGAAGAGTCGTCTTCATTCTTTCACTCAAATTGCCAAACATGGTATGTTATGTGTGGTCTTATGCATGATTTTGCAAGGATGATCTCAAGAACTGAGTGTGCAACTATAGATGGTTTACAGTGCAATGGGATGATGTCAACTGTGCGACATTTATCAATAGTAACTGACTCTGCATACAAGAAAGATCAGCATGGGAATATTCTTCGTAATGAGAAGTTCGAAGAATATCTAAGGAGTACAGTTACATCAGTTGGTAAATTAAGGACGTTGATTTTACTTGGGCACTATGACTCTTTCTTCTCACAGTTGTTCAAAGATATTTTCAAAGAGGCACATAATTTACACCTGCTGCAGATGTCTGCAACATCTGCTGATTTTAGTTCCTTCCTATGTGGTTTGGCAAGCGCGGTGCATCTTCGTTATCTAAAACTTGAGTCAGATGGGTTGGAGGGGGATTTTCCACAAGTTTTGGTCAATCTTTTTCATCTTCAGGTATTAGATGTTGGCTCAAACACCGATCCTATTTTACCTAATGGCATGCATAATCTTGTGAACCTGCGGTATCTTGTTGCAGAAAAGGGAGTATACTCTTCCATTGCTAGCATTGGTAGCATGACATCACTTCAACAACTTCATAATATTAAGGTTCAATTTTCTTGTATCGGCTTTGAGATAACACAACTCCAGTCTATGAACGAGCTTGTACAACTTGGTGTGTCTGAACTTGAAAATGTCAAAACTAGATATGAGGCTAATGGAGCAAAACTGAGAGACAAAAGACACTTAGAAGAGTTGCGCTTGTTGTGGACGCATACTCCGTCACGAGATGAATATGCCACTGACACGAGCTTTCAACATCCAGTGGACAATGTAGAAAGAGATGTAGAGCTCTTGCCAATGGTTGAAAGAGGGCCAAGTTCCGAGCCTTGTCTGGACAGAGCAAGAGAGGTGCTAGAGGGTCTTGAACCACATCAAGACTTAAAACATCTTCAGATATCTGGGTACTATGGTGCTACATCCCCAACTTGGCTTGCCAACAATATCTCAGTTACCTCCCTGCGAACCCTTCATCTAGACAGTTGTGGAGAATGGGAAATACTTCCGTTTATGGAAAGGTTTCCACTTCTGATAAAACTGAAGTTGACCAACCTGCGGAAAGTAATCGAAGTATTGGTTCCTTCACTGGAGGAGCTAGTTTTAGTTGAAATGCCAAAGTTGCAAAGATGTTTGTGCATTTCCGTGGGGGGTCTGAGCTCTAGCTTAAGGGCATTGCACATCGATAAGTGTCAAGCACTAAAGACGTTTGATCTGTTTATGAACGATCATAAAATCAAACTAGAGCAGAGGCCATGGTTGTCTGGTCTTAGGAAATTAATTATGCGTGATTGCCCTCATTTAAAAGTATTGAACCCTCTTCCACCTTCAGCCACCTTTTCTGAGTTACTCATCAGTGGAGTTTCAACACTTCCAAGTATGAAGGGGTCATCTAGTGAAACGTTACATATTGGATCTTTCAATTGGTTTATTGATCACTCTTCTGGTGAGTTGACGGTACTGGATGATAAAATATTGGCATTCCACAACCTGAGGAGAATCAAATTGATGAGAATATATGGTTGCCGGAATCTAACTTCTATTTCATTCGAAGGTTTTAGTCATCTCGTCTCTTTAGAGAGGTTGGAAATACACTGGTGCGAAAAATTGTTCTCTTCACATGTTTTTCCAGAGCATATCCTTGAAGATGTGCCGACTGCAAATTGCAAGGCCTTCCCTTCTCTTGAAAGTCTCACTATTGAGTTCTGTGGAATAGCAGGGAAGTGGCTATCTCTGATGCTGCAACATGCGCCAAACCTAGAAGAATTGATTTTAGAGAATTGCCCCCGTATAACAACGCTGTTATCGACAGAAGAGGAAGAAAACAGTCCATCAAATCTTATCATGGACAGGGGGTACTCGTCATCAGGAAATCTAGATGACGCATTGGCAGGGTTAGCTCAAGACGAACTCTTGCACGTTCCATCAAATCCCGTCTCCTCTCTTAGGAAGATAACTATTCAGGGCTGCCCTTGTCTGACATTTAATGGGAGCAAGAACGGCTTCTCTAGATTTACCTCCCTTGAGGAGATAACGATCTACAACTGCCCCGAGCTGTTCTCGCCTTTGGTGCATAAAGCCGGAAATGATGACCGCACAAACGGAAGATGGCTATTCCCAACATCACTTGGGGAACTTGACATCGACGGCTATTCCCAAGAGACGCTGCAGCCGTGTTTTCCAAGTCCTCTCACCAGCCTTAAAAAGTTGGAGGTACTGAGCAGCCCAGGTTTGGAATCTCTGCAGCTTCAGTCATGCACGGCACTTGAAGAGCTGATAATTGGAGGCTGTGGATCACTCACCGCACTAGAGGGCTTGCAATCCATTGGCAACCTCAGGCATTTGAAAGTATCTGATTGCCCTGGCCTGCCTCCATATTTAGAGAGCTTGTCAAGGCAGGGCTATGAGATCTGCCCTCGACTGGAAGGACTTCACATCGATGACCCATCTGTCCTTAGCAAGTCATTCTGCAAGCATCTCACCTCCCTCCAACGCCTAGAACTGGGTCATTTGAGCATGGAAGCGACAACACTGACTGATGAGCAAGAGAGAGCGCTTCTGCTGCTTAAGTCCCTGCAAGAGCTCGACATTTGTGGTTGTTATCATCTCGTAGATCTTCCTGCGAGGCTGGACACCCTTACTTCCCTCAATAGGTTCAAGATACATTCCTGCTCCATCATCTCAAGGCTCCCACTAGCATTTTAGCAGTACACATGTATTCCTGATGTTTTGTAATCAATAATTTGCCACAGACCTGCATGCACTAGGCTGCCCAGATTCTGTGACCACTGTCCCTCTGCTCTCCTAAACTTGGGCCATACATTATGTTATATTCAGAATTGATATACCCTCATAAATGTGCACTATGCTCAATGTAAAAAAGACCGTCTCTCTGCATATGATTCGGTCTTCAGACAATTTTCCTAAAGCCCTTCTATCAGTTGTAGCATGCTTTGCCGTATGCGTTAACAAAAGATTAACAAATGTACATGATAGCTGATGGTCTAATCAATCTTTCTATTGTGATCAGGATGT >curated_TraesCS2D01G466600MEAAIAWLVETILATLLIDKLDAWIRQAGLADDIEKLKSEIRRIKMVISALKGRGIRKEALAESLALLEDHLYVRRRRRGGRARLLQAPTAGPGTRGHSHCLAACRSKLASDERQGVDGAERVNEIPRGDAATRNSSVGKLRSLVWEHFTITQKDDGKPVKAKCTYCTEEFRCETKTNGTSSMRNHLEKEHSVICTKRPGAHPPNLSSTGEPIVIGSSSKGKGKKRRSKAWDSFDVTKEVNGQPIKARCKYCPTEIKCGTGNGTAGMLNHNKICKKKPGLDDQPPNSSSTNDTTANDATTNARPNLIGDSSSRKRRRVDEESAQNIAANTSTPWNKAELSNRIQQIISRLQDIRGEVSEVFKLHESDSASSLDHNRSTTSDQHLRTSSLISRQLYGRVAEKKSILKLMMSDDTSNSIIVLPIVGVAGVGKTALTQLVYNEPNVESRFQHRVWIWVSRNFDEVRITREMLNFVSREKHEEINCFVKLQEILKIHVKSKRVLIILDDVWDDMNDCRWNQLLAPFKFNSANGNVILVTTRKLSVAKMVGTTEPIKIGALEEDDFWLLFKSCALGDRASENPGNLCTIGRQIAGKLKGNPLAAVTAGALLRDHLTVDHWSNILKKEDWKSLGLSGGIMPALKLSYDELPYHLQRCLSYCSIFPNKHKFSGKDLVYIWISQGFVSCANLSKSLEEIGWQYLIDMTNMGLFQQVRGEESSSFFHSNCQTWYVMCGLMHDFARMISRTECATIDGLQCNGMMSTVRHLSIVTDSAYKKDQHGNILRNEKFEEYLRSTVTSVGKLRTLILLGHYDSFFSQLFKDIFKEAHNLHLLQMSATSADFSSFLCGLASAVHLRYLKLESDGLEGDFPQVLVNLFHLQVLDVGSNTDPILPNGMHNLVNLRYLVAEKGVYSSIASIGSMTSLQQLHNIKVQFSCIGFEITQLQSMNELVQLGVSELENVKTRYEANGAKLRDKRHLEELRLLWTHTPSRDEYATDTSFQHPVDNVERDVELLPMVERGPSSEPCLDRAREVLEGLEPHQDLKHLQISGYYGATSPTWLANNISVTSLRTLHLDSCGEWEILPFMERFPLLIKLKLTNLRKVIEVLVPSLEELVLVEMPKLQRCLCISVGGLSSSLRALHIDKCQALKTFDLFMNDHKIKLEQRPWLSGLRKLIMRDCPHLKVLNPLPPSATFSELLISGVSTLPSMKGSSSETLHIGSFNWFIDHSSGELTVLDDKILAFHNLRRIKLMRIYGCRNLTSISFEGFSHLVSLERLEIHWCEKLFSSHVFPEHILEDVPTANCKAFPSLESLTIEFCGIAGKWLSLMLQHAPNLEELILENCPRITTLLSTEEEENSPSNLIMDRGYSSSGNLDDALAGLAQDELLHVPSNPVSSLRKITIQGCPCLTFNGSKNGFSRFTSLEEITIYNCPELFSPLVHKAGNDDRTNGRWLFPTSLGELDIDGYSQETLQPCFPSPLTSLKKLEVLSSPGLESLQLQSCTALEELIIGGCGSLTALEGLQSIGNLRHLKVSDCPGLPPYLESLSRQGYEICPRLEGLHIDDPSVLSKSFCKHLTSLQRLELGHLSMEATTLTDEQERALLLLKSLQELDICGCYHLVDLPARLDTLTSLNRFKIHSCSIISRLPLAF-

1. An isolated nucleic acid encoding a nucleotide-binding andleucine-rich repeat (NLR) polypeptide comprising a zinc-finger BEDdomain, wherein expression of the NLR polypeptide in a plant confers orenhances resistance of the plant to a fungus.
 2. The isolated nucleicacid according to claim 1, wherein the nucleic acid is isolated from aplant.
 3. The isolated nucleic acid according to claim 1, wherein theBED domain has an amino acid sequence corresponding to SEQ ID NO: 1 or avariant or functional fragment thereof.
 4. The isolated nucleic acidaccording to claim 1, wherein the NLR polypeptide comprises aleucine-rich repeat (LRR) motif at or near the C-terminus.
 5. Theisolated nucleic acid according to claim 1, wherein the NLR polypeptidehas an amino acid sequence comprising SEQ ID NO: 2 or SEQ ID NO: 3, or avariant or functional fragment of either.
 6. The isolated nucleic acidaccording to claim 5, having a nucleotide sequence comprising SEQ ID NO:4 or SEQ ID NO:
 5. 7. The isolated nucleic acid taccording to claim 1,wherein the NLR polypeptide has an amino acid sequence comprising SEQ IDNO: 6 or a variant or functional fragment thereof.
 8. The isolatednucleic acid according to claim 7, having a nucleotide sequencecomprising SEQ ID NO:
 7. 9. The isolated nucleic acid according to claim1, wherein the NLR polypeptide comprises a further zinc-finger BEDdomain.
 10. A nucleotide-binding and leucine-rich repeat (NLR)polypeptide comprising a zinc-finger BED domain, wherein expression ofthe NLR polypeptide in a plant confers or enhances resistance of theplant to a fungus.
 11. The NLR polypeptide according to claim 10,wherein the BED domain has an amino acid sequence comprising SEQ ID NO:1 or a variant or functional fragment thereof.
 12. The NLR polypeptideaccording to claim 10, comprising a leucine-rich repeat (LRR) motif ator near the C-terminus.
 13. The NLR polypeptide according to claim 10,having an amino acid sequence comprising SEQ ID NO: 2 or SEQ ID NO: 3,or a variant or functional fragment of either.
 14. The NLR polypeptideaccording to claim 10, having an amino acid sequence comprising SEQ IDNO: 6 or a variant or functional fragment thereof.
 15. A vectorcomprising an isolated nucleic acid as defined in claim
 1. 16. Thevector according to claim 15, further comprising a regulatory sequencewhich directs expression of the nucleic acid.
 17. A host cell comprisinga nucleic acid as defined in claim 1, an NLR polypeptide or a vector.18. The host cell according to claim 17, which is a bacterial cell, ayeast cell or a plant cell.
 19. A method of producing a transgenic plantor plant cell comprising introducing and expressing a nucleic acidaccording to claim 1 or a vector into a plant or plant cell, whereinintroducing and expressing the nucleic acid or vector confers orenhances resistance of the plant or plant cell to a fungal pathogen suchas wheat yellow (stripe) rust fungus Puccinia striiformisi f. sp.tritici.
 20. The method of claim 19, wherein the transgenic plant orplant cell has resistance or enhanced resistance to the fungal pathogencompared to a plant or plant cell of the same species lacking thenucleic acid or vector.
 21. A method for producing a non-transgenicplant or plant cell having resistance or enhanced resistance to a fungalpathogen, the method comprising mutating or editing the genomic materialof the plant or plant cell to comprise a nucleic acid as defined inclaim
 1. 22. A plant or plant cell obtained or obtainable by the methodas defined in claim
 19. 23. The plant or plant cell of claim 22, whereinthe plant or plant cell is a crop plant or plant cell or a biofuel plantor plant cell.
 24. A seed of the plant of claim 22, wherein the seedcomprises a nucleic acid or an NLR polypeptide
 25. The seed according toclaim 24, which is a wheat seed.
 26. A method of limiting wheat yellow(stripe) rust in agricultural crop production, the method comprisingplanting a wheat seed as defined in claim 25 and growing a wheat plantunder conditions favourable for the growth and development of the wheatplant.
 27. A method for identification or selection of an organism suchas plant having resistance to a fungus such as wheat yellow (stripe)rust fungus Puccinia striiformisi f. sp. tritici, comprising the step ofscreening the organism for the presence or absence of: (1) a nucleicacid as defined in claim 1; and/or (2) an NLR polypeptide, whereinpresence of the nucleic acid or the NLR polypeptide indicatesresistance.